Strain Fitness in Escherichia coli HS(pFamp)R (ATCC 700891) around OHPLBJKB_00645

Experiment: T3 MOI1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOHPLBJKB_00642 and OHPLBJKB_00643 are separated by 155 nucleotidesOHPLBJKB_00643 and OHPLBJKB_00644 are separated by 181 nucleotidesOHPLBJKB_00644 and OHPLBJKB_00645 are separated by 12 nucleotidesOHPLBJKB_00645 and OHPLBJKB_00646 are separated by 104 nucleotidesOHPLBJKB_00646 and OHPLBJKB_00647 are separated by 89 nucleotides OHPLBJKB_00642: OHPLBJKB_00642 - IS1 family transposase IS1S, at 660,977 to 661,243 _00642 OHPLBJKB_00643: OHPLBJKB_00643 - IS1 family transposase IS1A, at 661,399 to 661,674 _00643 OHPLBJKB_00644: OHPLBJKB_00644 - HTH-type transcriptional regulator DmlR, at 661,856 to 662,188 _00644 OHPLBJKB_00645: OHPLBJKB_00645 - putative glycerol-3-phosphate acyltransferase, at 662,201 to 662,818 _00645 OHPLBJKB_00646: OHPLBJKB_00646 - Dihydroneopterin aldolase, at 662,923 to 663,291 _00646 OHPLBJKB_00647: OHPLBJKB_00647 - Undecaprenyl-diphosphatase, at 663,381 to 664,202 _00647 Position (kb) 662 663Strain fitness (log2 ratio) -1 0 1at 661.699 kb on + strandat 661.699 kb on + strandat 661.758 kb on + strandat 661.758 kb on + strandat 661.759 kb on - strandat 661.759 kb on - strandat 661.761 kb on + strandat 661.762 kb on - strandat 661.762 kb on - strandat 661.779 kb on - strandat 661.818 kb on + strandat 661.818 kb on + strandat 661.818 kb on + strandat 661.819 kb on - strandat 661.819 kb on - strandat 662.038 kb on - strand, within OHPLBJKB_00644at 662.048 kb on + strand, within OHPLBJKB_00644at 662.109 kb on + strand, within OHPLBJKB_00644at 662.109 kb on + strand, within OHPLBJKB_00644at 662.110 kb on - strand, within OHPLBJKB_00644at 662.110 kb on - strand, within OHPLBJKB_00644at 662.186 kb on + strandat 662.186 kb on + strandat 662.186 kb on + strandat 662.187 kb on - strandat 662.187 kb on - strandat 662.187 kb on - strandat 662.187 kb on - strandat 662.344 kb on + strand, within OHPLBJKB_00645at 662.345 kb on - strand, within OHPLBJKB_00645at 662.358 kb on + strand, within OHPLBJKB_00645at 662.358 kb on + strand, within OHPLBJKB_00645at 662.359 kb on - strand, within OHPLBJKB_00645at 662.414 kb on + strand, within OHPLBJKB_00645at 662.415 kb on - strand, within OHPLBJKB_00645at 662.444 kb on + strand, within OHPLBJKB_00645at 662.445 kb on - strand, within OHPLBJKB_00645at 662.445 kb on - strand, within OHPLBJKB_00645at 662.564 kb on + strand, within OHPLBJKB_00645at 662.564 kb on + strand, within OHPLBJKB_00645at 662.564 kb on + strand, within OHPLBJKB_00645at 662.565 kb on - strand, within OHPLBJKB_00645at 662.565 kb on - strand, within OHPLBJKB_00645at 662.565 kb on - strand, within OHPLBJKB_00645at 662.591 kb on + strand, within OHPLBJKB_00645at 662.598 kb on + strand, within OHPLBJKB_00645at 662.675 kb on - strand, within OHPLBJKB_00645at 662.679 kb on - strand, within OHPLBJKB_00645at 662.782 kb on - strandat 662.818 kb on + strandat 662.818 kb on + strandat 662.818 kb on + strandat 662.819 kb on - strandat 662.819 kb on - strandat 662.819 kb on - strandat 662.868 kb on + strandat 662.868 kb on + strandat 662.868 kb on + strandat 662.869 kb on - strandat 663.296 kb on - strandat 663.319 kb on + strandat 663.320 kb on - strandat 663.320 kb on - strandat 663.320 kb on - strandat 663.337 kb on + strandat 663.337 kb on + strandat 663.338 kb on - strandat 663.353 kb on + strandat 663.363 kb on + strandat 663.364 kb on - strandat 663.376 kb on + strandat 663.376 kb on + strandat 663.377 kb on - strandat 663.390 kb on - strandat 663.390 kb on - strandat 663.471 kb on - strand, within OHPLBJKB_00647at 663.476 kb on + strand, within OHPLBJKB_00647at 663.476 kb on + strand, within OHPLBJKB_00647at 663.476 kb on + strand, within OHPLBJKB_00647at 663.541 kb on + strand, within OHPLBJKB_00647at 663.541 kb on + strand, within OHPLBJKB_00647at 663.542 kb on - strand, within OHPLBJKB_00647at 663.542 kb on - strand, within OHPLBJKB_00647at 663.559 kb on + strand, within OHPLBJKB_00647at 663.562 kb on + strand, within OHPLBJKB_00647at 663.562 kb on + strand, within OHPLBJKB_00647at 663.562 kb on + strand, within OHPLBJKB_00647at 663.562 kb on + strand, within OHPLBJKB_00647at 663.563 kb on - strand, within OHPLBJKB_00647at 663.691 kb on + strand, within OHPLBJKB_00647at 663.720 kb on - strand, within OHPLBJKB_00647at 663.720 kb on - strand, within OHPLBJKB_00647

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Per-strain Table

Position Strand Gene LocusTag Fraction T3 MOI1
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661,699 + +0.4
661,699 + +0.4
661,758 + +0.4
661,758 + +0.2
661,759 - -0.4
661,759 - -1.2
661,761 + +0.1
661,762 - -1.5
661,762 - +1.0
661,779 - -0.2
661,818 + -0.4
661,818 + -0.2
661,818 + +0.9
661,819 - +0.7
661,819 - +0.5
662,038 - OHPLBJKB_00644 0.55 -0.4
662,048 + OHPLBJKB_00644 0.58 -0.2
662,109 + OHPLBJKB_00644 0.76 +0.5
662,109 + OHPLBJKB_00644 0.76 +0.7
662,110 - OHPLBJKB_00644 0.76 +0.2
662,110 - OHPLBJKB_00644 0.76 +0.5
662,186 + -0.5
662,186 + -0.3
662,186 + +0.4
662,187 - +0.0
662,187 - -1.7
662,187 - +0.9
662,187 - +0.5
662,344 + OHPLBJKB_00645 0.23 -1.1
662,345 - OHPLBJKB_00645 0.23 +0.0
662,358 + OHPLBJKB_00645 0.25 +0.2
662,358 + OHPLBJKB_00645 0.25 -0.8
662,359 - OHPLBJKB_00645 0.26 -0.2
662,414 + OHPLBJKB_00645 0.34 +0.1
662,415 - OHPLBJKB_00645 0.35 +0.1
662,444 + OHPLBJKB_00645 0.39 -1.6
662,445 - OHPLBJKB_00645 0.39 -0.5
662,445 - OHPLBJKB_00645 0.39 -1.2
662,564 + OHPLBJKB_00645 0.59 -0.7
662,564 + OHPLBJKB_00645 0.59 +1.3
662,564 + OHPLBJKB_00645 0.59 -1.7
662,565 - OHPLBJKB_00645 0.59 -0.9
662,565 - OHPLBJKB_00645 0.59 -0.2
662,565 - OHPLBJKB_00645 0.59 +0.7
662,591 + OHPLBJKB_00645 0.63 -0.7
662,598 + OHPLBJKB_00645 0.64 +0.6
662,675 - OHPLBJKB_00645 0.77 -0.2
662,679 - OHPLBJKB_00645 0.77 -0.0
662,782 - +0.4
662,818 + -0.2
662,818 + +0.1
662,818 + +0.3
662,819 - -0.2
662,819 - -0.6
662,819 - +0.4
662,868 + -0.0
662,868 + -1.3
662,868 + +0.6
662,869 - -0.5
663,296 - +0.7
663,319 + -0.2
663,320 - -1.0
663,320 - -0.6
663,320 - +0.2
663,337 + -0.8
663,337 + -0.6
663,338 - +1.3
663,353 + -0.4
663,363 + -0.4
663,364 - +0.6
663,376 + -0.1
663,376 + -0.2
663,377 - -0.5
663,390 - -0.6
663,390 - +0.6
663,471 - OHPLBJKB_00647 0.11 +1.4
663,476 + OHPLBJKB_00647 0.12 -0.0
663,476 + OHPLBJKB_00647 0.12 -0.1
663,476 + OHPLBJKB_00647 0.12 +0.8
663,541 + OHPLBJKB_00647 0.19 -0.4
663,541 + OHPLBJKB_00647 0.19 +1.2
663,542 - OHPLBJKB_00647 0.20 -0.0
663,542 - OHPLBJKB_00647 0.20 -0.4
663,559 + OHPLBJKB_00647 0.22 -0.3
663,562 + OHPLBJKB_00647 0.22 -0.8
663,562 + OHPLBJKB_00647 0.22 -0.1
663,562 + OHPLBJKB_00647 0.22 -0.3
663,562 + OHPLBJKB_00647 0.22 +0.7
663,563 - OHPLBJKB_00647 0.22 -0.4
663,691 + OHPLBJKB_00647 0.38 -0.2
663,720 - OHPLBJKB_00647 0.41 +0.5
663,720 - OHPLBJKB_00647 0.41 +0.7

Or see this region's nucleotide sequence