Strain Fitness in Escherichia coli HS(pFamp)R (ATCC 700891) around OHPLBJKB_04217

Experiment: N4 MOI10

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntOHPLBJKB_04216 and OHPLBJKB_04217 overlap by 4 nucleotidesOHPLBJKB_04217 and OHPLBJKB_04218 are separated by 13 nucleotidesOHPLBJKB_04218 and OHPLBJKB_04219 are separated by 94 nucleotides OHPLBJKB_04216: OHPLBJKB_04216 - putative protein kinase UbiB, at 4,433,775 to 4,435,415 _04216 OHPLBJKB_04217: OHPLBJKB_04217 - hypothetical protein, at 4,435,412 to 4,436,017 _04217 OHPLBJKB_04218: OHPLBJKB_04218 - Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE, at 4,436,031 to 4,436,786 _04218 OHPLBJKB_04219: OHPLBJKB_04219 - hypothetical protein, at 4,436,881 to 4,438,308 _04219 Position (kb) 4435 4436 4437Strain fitness (log2 ratio) -1 0 1at 4435.520 kb on - strand, within OHPLBJKB_04217at 4435.842 kb on - strand, within OHPLBJKB_04217at 4435.842 kb on - strand, within OHPLBJKB_04217at 4435.911 kb on - strand, within OHPLBJKB_04217at 4435.911 kb on - strand, within OHPLBJKB_04217at 4436.040 kb on - strandat 4436.040 kb on - strandat 4436.869 kb on - strandat 4436.898 kb on + strandat 4436.898 kb on + strandat 4436.898 kb on + strandat 4436.899 kb on - strandat 4436.900 kb on + strandat 4436.900 kb on + strandat 4436.919 kb on - strandat 4436.919 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction N4 MOI10
remove
4,435,520 - OHPLBJKB_04217 0.18 -0.2
4,435,842 - OHPLBJKB_04217 0.71 -1.7
4,435,842 - OHPLBJKB_04217 0.71 -0.4
4,435,911 - OHPLBJKB_04217 0.82 +0.1
4,435,911 - OHPLBJKB_04217 0.82 +0.1
4,436,040 - -0.5
4,436,040 - +0.4
4,436,869 - +0.2
4,436,898 + -0.1
4,436,898 + -0.8
4,436,898 + -0.1
4,436,899 - +0.8
4,436,900 + -0.5
4,436,900 + -0.7
4,436,919 - -0.9
4,436,919 - +0.6

Or see this region's nucleotide sequence