Experiment: N4 MOI10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OHPLBJKB_04216 and OHPLBJKB_04217 overlap by 4 nucleotides OHPLBJKB_04217 and OHPLBJKB_04218 are separated by 13 nucleotides OHPLBJKB_04218 and OHPLBJKB_04219 are separated by 94 nucleotides
OHPLBJKB_04216: OHPLBJKB_04216 - putative protein kinase UbiB, at 4,433,775 to 4,435,415
_04216
OHPLBJKB_04217: OHPLBJKB_04217 - hypothetical protein, at 4,435,412 to 4,436,017
_04217
OHPLBJKB_04218: OHPLBJKB_04218 - Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE, at 4,436,031 to 4,436,786
_04218
OHPLBJKB_04219: OHPLBJKB_04219 - hypothetical protein, at 4,436,881 to 4,438,308
_04219
Position (kb)
4435
4436
4437 Strain fitness (log2 ratio)
-1
0
1 at 4435.520 kb on - strand, within OHPLBJKB_04217 at 4435.842 kb on - strand, within OHPLBJKB_04217 at 4435.842 kb on - strand, within OHPLBJKB_04217 at 4435.911 kb on - strand, within OHPLBJKB_04217 at 4435.911 kb on - strand, within OHPLBJKB_04217 at 4436.040 kb on - strand at 4436.040 kb on - strand at 4436.869 kb on - strand at 4436.898 kb on + strand at 4436.898 kb on + strand at 4436.898 kb on + strand at 4436.899 kb on - strand at 4436.900 kb on + strand at 4436.900 kb on + strand at 4436.919 kb on - strand at 4436.919 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N4 MOI10 remove 4,435,520 - OHPLBJKB_04217 0.18 -0.2 4,435,842 - OHPLBJKB_04217 0.71 -1.7 4,435,842 - OHPLBJKB_04217 0.71 -0.4 4,435,911 - OHPLBJKB_04217 0.82 +0.1 4,435,911 - OHPLBJKB_04217 0.82 +0.1 4,436,040 - -0.5 4,436,040 - +0.4 4,436,869 - +0.2 4,436,898 + -0.1 4,436,898 + -0.8 4,436,898 + -0.1 4,436,899 - +0.8 4,436,900 + -0.5 4,436,900 + -0.7 4,436,919 - -0.9 4,436,919 - +0.6
Or see this region's nucleotide sequence