Strain Fitness in Escherichia coli HS(pFamp)R (ATCC 700891) around OHPLBJKB_02536

Experiment: N4 MOI10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOHPLBJKB_02535 and OHPLBJKB_02536 are separated by 35 nucleotidesOHPLBJKB_02536 and OHPLBJKB_02537 are separated by 3 nucleotides OHPLBJKB_02535: OHPLBJKB_02535 - tRNA-specific 2-thiouridylase MnmA, at 2,646,452 to 2,647,558 _02535 OHPLBJKB_02536: OHPLBJKB_02536 - High frequency lysogenization protein HflD, at 2,647,594 to 2,648,235 _02536 OHPLBJKB_02537: OHPLBJKB_02537 - Adenylosuccinate lyase, at 2,648,239 to 2,649,609 _02537 Position (kb) 2647 2648 2649Strain fitness (log2 ratio) -1 0 1at 2647.569 kb on + strandat 2647.573 kb on + strandat 2647.606 kb on + strandat 2647.609 kb on + strandat 2647.700 kb on + strand, within OHPLBJKB_02536at 2647.722 kb on + strand, within OHPLBJKB_02536at 2647.722 kb on + strand, within OHPLBJKB_02536at 2647.835 kb on + strand, within OHPLBJKB_02536at 2648.005 kb on + strand, within OHPLBJKB_02536at 2648.005 kb on + strand, within OHPLBJKB_02536at 2648.005 kb on + strand, within OHPLBJKB_02536at 2648.005 kb on + strand, within OHPLBJKB_02536at 2648.005 kb on + strand, within OHPLBJKB_02536at 2648.005 kb on + strand, within OHPLBJKB_02536at 2648.063 kb on + strand, within OHPLBJKB_02536

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Per-strain Table

Position Strand Gene LocusTag Fraction N4 MOI10
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2,647,569 + -0.1
2,647,573 + +0.6
2,647,606 + +0.2
2,647,609 + -0.8
2,647,700 + OHPLBJKB_02536 0.17 -0.0
2,647,722 + OHPLBJKB_02536 0.20 -1.2
2,647,722 + OHPLBJKB_02536 0.20 +0.2
2,647,835 + OHPLBJKB_02536 0.38 -0.8
2,648,005 + OHPLBJKB_02536 0.64 +0.3
2,648,005 + OHPLBJKB_02536 0.64 -1.6
2,648,005 + OHPLBJKB_02536 0.64 -0.7
2,648,005 + OHPLBJKB_02536 0.64 -1.2
2,648,005 + OHPLBJKB_02536 0.64 -0.4
2,648,005 + OHPLBJKB_02536 0.64 +0.2
2,648,063 + OHPLBJKB_02536 0.73 +0.3

Or see this region's nucleotide sequence