Experiment: N4 MOI10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OHPLBJKB_01076 and OHPLBJKB_01077 are separated by 254 nucleotides OHPLBJKB_01077 and OHPLBJKB_01078 are separated by 61 nucleotides OHPLBJKB_01078 and OHPLBJKB_01079 are separated by 229 nucleotides OHPLBJKB_01079 and OHPLBJKB_01080 are separated by 155 nucleotides
OHPLBJKB_01076: OHPLBJKB_01076 - hypothetical protein, at 1,108,192 to 1,108,470
_01076
OHPLBJKB_01077: OHPLBJKB_01077 - IS3 family transposase ISEhe3, at 1,108,725 to 1,109,339
_01077
OHPLBJKB_01078: OHPLBJKB_01078 - hypothetical protein, at 1,109,401 to 1,109,883
_01078
OHPLBJKB_01079: OHPLBJKB_01079 - IS1 family transposase IS1A, at 1,110,113 to 1,110,388
_01079
OHPLBJKB_01080: OHPLBJKB_01080 - IS1 family transposase IS1S, at 1,110,544 to 1,110,810
_01080
Position (kb)
1109
1110 Strain fitness (log2 ratio)
-1
0
1 at 1109.384 kb on + strand at 1109.385 kb on - strand at 1109.409 kb on + strand at 1109.546 kb on - strand, within OHPLBJKB_01078 at 1109.590 kb on - strand, within OHPLBJKB_01078 at 1109.590 kb on - strand, within OHPLBJKB_01078 at 1109.715 kb on - strand, within OHPLBJKB_01078 at 1109.742 kb on - strand, within OHPLBJKB_01078 at 1109.901 kb on - strand at 1109.955 kb on + strand at 1110.057 kb on - strand at 1110.057 kb on - strand at 1110.842 kb on + strand at 1110.842 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction N4 MOI10 remove 1,109,384 + +0.5 1,109,385 - -0.2 1,109,409 + -1.1 1,109,546 - OHPLBJKB_01078 0.30 -0.6 1,109,590 - OHPLBJKB_01078 0.39 -1.0 1,109,590 - OHPLBJKB_01078 0.39 -0.2 1,109,715 - OHPLBJKB_01078 0.65 -0.6 1,109,742 - OHPLBJKB_01078 0.71 -1.0 1,109,901 - +0.4 1,109,955 + -1.3 1,110,057 - +0.8 1,110,057 - -1.1 1,110,842 + +0.5 1,110,842 + -0.1
Or see this region's nucleotide sequence