Strain Fitness in Escherichia coli HS(pFamp)R (ATCC 700891) around OHPLBJKB_01078

Experiment: N4 MOI10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOHPLBJKB_01076 and OHPLBJKB_01077 are separated by 254 nucleotidesOHPLBJKB_01077 and OHPLBJKB_01078 are separated by 61 nucleotidesOHPLBJKB_01078 and OHPLBJKB_01079 are separated by 229 nucleotidesOHPLBJKB_01079 and OHPLBJKB_01080 are separated by 155 nucleotides OHPLBJKB_01076: OHPLBJKB_01076 - hypothetical protein, at 1,108,192 to 1,108,470 _01076 OHPLBJKB_01077: OHPLBJKB_01077 - IS3 family transposase ISEhe3, at 1,108,725 to 1,109,339 _01077 OHPLBJKB_01078: OHPLBJKB_01078 - hypothetical protein, at 1,109,401 to 1,109,883 _01078 OHPLBJKB_01079: OHPLBJKB_01079 - IS1 family transposase IS1A, at 1,110,113 to 1,110,388 _01079 OHPLBJKB_01080: OHPLBJKB_01080 - IS1 family transposase IS1S, at 1,110,544 to 1,110,810 _01080 Position (kb) 1109 1110Strain fitness (log2 ratio) -1 0 1at 1109.384 kb on + strandat 1109.385 kb on - strandat 1109.409 kb on + strandat 1109.546 kb on - strand, within OHPLBJKB_01078at 1109.590 kb on - strand, within OHPLBJKB_01078at 1109.590 kb on - strand, within OHPLBJKB_01078at 1109.715 kb on - strand, within OHPLBJKB_01078at 1109.742 kb on - strand, within OHPLBJKB_01078at 1109.901 kb on - strandat 1109.955 kb on + strandat 1110.057 kb on - strandat 1110.057 kb on - strandat 1110.842 kb on + strandat 1110.842 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N4 MOI10
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1,109,384 + +0.5
1,109,385 - -0.2
1,109,409 + -1.1
1,109,546 - OHPLBJKB_01078 0.30 -0.6
1,109,590 - OHPLBJKB_01078 0.39 -1.0
1,109,590 - OHPLBJKB_01078 0.39 -0.2
1,109,715 - OHPLBJKB_01078 0.65 -0.6
1,109,742 - OHPLBJKB_01078 0.71 -1.0
1,109,901 - +0.4
1,109,955 + -1.3
1,110,057 - +0.8
1,110,057 - -1.1
1,110,842 + +0.5
1,110,842 + -0.1

Or see this region's nucleotide sequence