Experiment: Pullulan 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BACOVA_04281 and BACOVA_04282 are separated by 4 nucleotides BACOVA_04282 and BACOVA_04283 are separated by 8 nucleotides BACOVA_04283 and BACOVA_04284 are separated by 450 nucleotides BACOVA_04284 and BACOVA_04285 overlap by 8 nucleotides BACOVA_04285 and BACOVA_04286 are separated by 3 nucleotides BACOVA_04286 and BACOVA_04287 are separated by 128 nucleotides
BACOVA_04281: BACOVA_04281 - ABC transporter, ATP-binding protein, at 168,880 to 170,514
_04281
BACOVA_04282: BACOVA_04282 - hypothetical protein, at 170,519 to 170,665
_04282
BACOVA_04283: BACOVA_04283 - hypothetical protein, at 170,674 to 170,796
_04283
BACOVA_04284: BACOVA_04284 - hydrolase, NUDIX family, at 171,247 to 172,035
_04284
BACOVA_04285: BACOVA_04285 - cyclic nucleotide-binding domain protein, at 172,028 to 172,438
_04285
BACOVA_04286: BACOVA_04286 - hypothetical protein, at 172,442 to 172,606
_04286
BACOVA_04287: BACOVA_04287 - acyltransferase, at 172,735 to 173,742
_04287
Position (kb)
171
172
173 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 170.248 kb on + strand, within BACOVA_04281 at 170.340 kb on + strand, within BACOVA_04281 at 170.347 kb on - strand, within BACOVA_04281 at 170.356 kb on - strand at 170.376 kb on + strand at 170.377 kb on - strand at 170.377 kb on - strand at 170.377 kb on - strand at 170.393 kb on + strand at 170.419 kb on - strand at 170.447 kb on + strand at 170.504 kb on + strand at 170.504 kb on + strand at 170.512 kb on + strand at 170.513 kb on - strand at 170.513 kb on - strand at 170.517 kb on + strand at 170.517 kb on + strand at 170.518 kb on - strand at 170.553 kb on + strand, within BACOVA_04282 at 170.554 kb on - strand, within BACOVA_04282 at 170.583 kb on + strand, within BACOVA_04282 at 170.584 kb on - strand, within BACOVA_04282 at 170.658 kb on + strand at 170.658 kb on + strand at 170.698 kb on - strand, within BACOVA_04283 at 170.714 kb on - strand, within BACOVA_04283 at 170.778 kb on + strand, within BACOVA_04283 at 170.779 kb on - strand, within BACOVA_04283 at 170.813 kb on - strand at 170.838 kb on - strand at 171.169 kb on + strand at 171.182 kb on - strand at 171.238 kb on - strand at 171.276 kb on + strand at 171.323 kb on + strand at 171.323 kb on + strand at 171.323 kb on + strand at 171.323 kb on + strand at 171.324 kb on - strand at 171.324 kb on - strand at 171.324 kb on - strand at 171.324 kb on - strand at 171.354 kb on + strand, within BACOVA_04284 at 171.355 kb on - strand, within BACOVA_04284 at 171.361 kb on - strand, within BACOVA_04284 at 171.388 kb on - strand, within BACOVA_04284 at 171.394 kb on - strand, within BACOVA_04284 at 171.398 kb on + strand, within BACOVA_04284 at 171.484 kb on + strand, within BACOVA_04284 at 171.490 kb on + strand, within BACOVA_04284 at 171.490 kb on + strand, within BACOVA_04284 at 171.491 kb on - strand, within BACOVA_04284 at 171.491 kb on - strand, within BACOVA_04284 at 171.638 kb on + strand, within BACOVA_04284 at 171.639 kb on - strand, within BACOVA_04284 at 171.663 kb on - strand, within BACOVA_04284 at 171.668 kb on + strand, within BACOVA_04284 at 171.668 kb on + strand, within BACOVA_04284 at 171.668 kb on + strand, within BACOVA_04284 at 171.783 kb on - strand, within BACOVA_04284 at 171.833 kb on - strand, within BACOVA_04284 at 171.840 kb on + strand, within BACOVA_04284 at 171.869 kb on + strand, within BACOVA_04284 at 171.893 kb on - strand, within BACOVA_04284 at 171.946 kb on + strand, within BACOVA_04284 at 172.016 kb on + strand at 172.017 kb on - strand at 172.074 kb on - strand, within BACOVA_04285 at 172.076 kb on + strand, within BACOVA_04285 at 172.104 kb on + strand, within BACOVA_04285 at 172.105 kb on - strand, within BACOVA_04285 at 172.115 kb on + strand, within BACOVA_04285 at 172.118 kb on - strand, within BACOVA_04285 at 172.184 kb on + strand, within BACOVA_04285 at 172.189 kb on + strand, within BACOVA_04285 at 172.221 kb on + strand, within BACOVA_04285 at 172.221 kb on + strand, within BACOVA_04285 at 172.222 kb on - strand, within BACOVA_04285 at 172.268 kb on - strand, within BACOVA_04285 at 172.268 kb on - strand, within BACOVA_04285 at 172.290 kb on + strand, within BACOVA_04285 at 172.298 kb on - strand, within BACOVA_04285 at 172.317 kb on - strand, within BACOVA_04285 at 172.327 kb on - strand, within BACOVA_04285 at 172.345 kb on - strand, within BACOVA_04285 at 172.345 kb on - strand, within BACOVA_04285 at 172.346 kb on + strand, within BACOVA_04285 at 172.347 kb on - strand, within BACOVA_04285 at 172.347 kb on - strand, within BACOVA_04285 at 172.354 kb on - strand, within BACOVA_04285 at 172.379 kb on + strand, within BACOVA_04285 at 172.387 kb on + strand, within BACOVA_04285 at 172.388 kb on - strand, within BACOVA_04285 at 172.393 kb on - strand, within BACOVA_04285 at 172.437 kb on - strand at 172.437 kb on - strand at 172.447 kb on + strand at 172.469 kb on + strand, within BACOVA_04286 at 172.469 kb on + strand, within BACOVA_04286 at 172.490 kb on + strand, within BACOVA_04286 at 172.491 kb on - strand, within BACOVA_04286 at 172.491 kb on - strand, within BACOVA_04286 at 172.545 kb on - strand, within BACOVA_04286 at 172.546 kb on + strand, within BACOVA_04286 at 172.546 kb on + strand, within BACOVA_04286 at 172.547 kb on - strand, within BACOVA_04286 at 172.547 kb on - strand, within BACOVA_04286 at 172.548 kb on + strand, within BACOVA_04286 at 172.549 kb on - strand, within BACOVA_04286 at 172.561 kb on - strand, within BACOVA_04286 at 172.631 kb on + strand at 172.633 kb on + strand at 172.649 kb on + strand at 172.649 kb on + strand at 172.650 kb on - strand at 172.685 kb on + strand at 172.686 kb on - strand at 172.725 kb on - strand at 172.739 kb on + strand at 172.740 kb on - strand at 172.756 kb on + strand at 172.756 kb on + strand at 172.756 kb on + strand at 172.756 kb on + strand at 172.756 kb on + strand at 172.757 kb on - strand at 172.843 kb on - strand, within BACOVA_04287 at 172.856 kb on - strand, within BACOVA_04287 at 172.869 kb on - strand, within BACOVA_04287 at 172.894 kb on - strand, within BACOVA_04287 at 172.941 kb on + strand, within BACOVA_04287 at 172.942 kb on - strand, within BACOVA_04287 at 172.946 kb on + strand, within BACOVA_04287 at 172.947 kb on - strand, within BACOVA_04287 at 172.962 kb on + strand, within BACOVA_04287 at 172.962 kb on + strand, within BACOVA_04287 at 172.962 kb on + strand, within BACOVA_04287 at 172.963 kb on - strand, within BACOVA_04287 at 172.963 kb on - strand, within BACOVA_04287 at 172.965 kb on - strand, within BACOVA_04287 at 172.989 kb on + strand, within BACOVA_04287
Per-strain Table
Position Strand Gene LocusTag Fraction Pullulan 2.5 mg/mL (C) remove 170,248 + BACOVA_04281 0.84 -0.7 170,340 + BACOVA_04281 0.89 +1.5 170,347 - BACOVA_04281 0.90 -0.7 170,356 - -1.4 170,376 + -1.3 170,377 - +1.3 170,377 - +0.3 170,377 - +0.0 170,393 + +0.0 170,419 - +1.2 170,447 + +0.6 170,504 + +2.4 170,504 + -2.5 170,512 + -1.7 170,513 - -0.4 170,513 - -2.3 170,517 + -0.9 170,517 + -0.8 170,518 - -0.4 170,553 + BACOVA_04282 0.23 +0.6 170,554 - BACOVA_04282 0.24 -1.4 170,583 + BACOVA_04282 0.44 +1.8 170,584 - BACOVA_04282 0.44 -1.6 170,658 + +0.7 170,658 + +1.0 170,698 - BACOVA_04283 0.20 -2.2 170,714 - BACOVA_04283 0.33 -0.7 170,778 + BACOVA_04283 0.85 -1.7 170,779 - BACOVA_04283 0.85 -0.1 170,813 - +0.6 170,838 - -1.7 171,169 + +0.2 171,182 - -3.4 171,238 - +0.0 171,276 + +3.6 171,323 + -0.5 171,323 + +1.3 171,323 + -0.3 171,323 + +0.8 171,324 - -2.7 171,324 - +0.8 171,324 - -0.1 171,324 - +0.8 171,354 + BACOVA_04284 0.14 -0.6 171,355 - BACOVA_04284 0.14 +1.6 171,361 - BACOVA_04284 0.14 -0.7 171,388 - BACOVA_04284 0.18 +0.9 171,394 - BACOVA_04284 0.19 +0.6 171,398 + BACOVA_04284 0.19 -4.2 171,484 + BACOVA_04284 0.30 +0.8 171,490 + BACOVA_04284 0.31 -2.4 171,490 + BACOVA_04284 0.31 -1.0 171,491 - BACOVA_04284 0.31 -1.8 171,491 - BACOVA_04284 0.31 +0.3 171,638 + BACOVA_04284 0.50 -1.8 171,639 - BACOVA_04284 0.50 -0.7 171,663 - BACOVA_04284 0.53 +1.0 171,668 + BACOVA_04284 0.53 -0.3 171,668 + BACOVA_04284 0.53 -0.2 171,668 + BACOVA_04284 0.53 +1.6 171,783 - BACOVA_04284 0.68 -2.1 171,833 - BACOVA_04284 0.74 -2.0 171,840 + BACOVA_04284 0.75 +1.3 171,869 + BACOVA_04284 0.79 -1.8 171,893 - BACOVA_04284 0.82 -0.9 171,946 + BACOVA_04284 0.89 +0.0 172,016 + +4.0 172,017 - +0.6 172,074 - BACOVA_04285 0.11 -0.7 172,076 + BACOVA_04285 0.12 +2.3 172,104 + BACOVA_04285 0.18 -1.9 172,105 - BACOVA_04285 0.19 +0.0 172,115 + BACOVA_04285 0.21 -0.3 172,118 - BACOVA_04285 0.22 -1.5 172,184 + BACOVA_04285 0.38 -0.6 172,189 + BACOVA_04285 0.39 +0.4 172,221 + BACOVA_04285 0.47 -1.0 172,221 + BACOVA_04285 0.47 +1.6 172,222 - BACOVA_04285 0.47 -1.2 172,268 - BACOVA_04285 0.58 +1.5 172,268 - BACOVA_04285 0.58 +0.1 172,290 + BACOVA_04285 0.64 -0.9 172,298 - BACOVA_04285 0.66 -2.3 172,317 - BACOVA_04285 0.70 -1.3 172,327 - BACOVA_04285 0.73 -0.2 172,345 - BACOVA_04285 0.77 +0.3 172,345 - BACOVA_04285 0.77 -0.8 172,346 + BACOVA_04285 0.77 +0.5 172,347 - BACOVA_04285 0.78 -1.3 172,347 - BACOVA_04285 0.78 -0.9 172,354 - BACOVA_04285 0.79 -0.6 172,379 + BACOVA_04285 0.85 -0.2 172,387 + BACOVA_04285 0.87 +0.8 172,388 - BACOVA_04285 0.88 -4.1 172,393 - BACOVA_04285 0.89 -0.7 172,437 - -0.6 172,437 - +0.3 172,447 + +2.8 172,469 + BACOVA_04286 0.16 -0.2 172,469 + BACOVA_04286 0.16 +0.6 172,490 + BACOVA_04286 0.29 +1.1 172,491 - BACOVA_04286 0.30 -1.1 172,491 - BACOVA_04286 0.30 -2.0 172,545 - BACOVA_04286 0.62 -0.6 172,546 + BACOVA_04286 0.63 +0.7 172,546 + BACOVA_04286 0.63 -0.4 172,547 - BACOVA_04286 0.64 -2.2 172,547 - BACOVA_04286 0.64 -0.7 172,548 + BACOVA_04286 0.64 -2.9 172,549 - BACOVA_04286 0.65 +0.3 172,561 - BACOVA_04286 0.72 +0.3 172,631 + +1.0 172,633 + +0.5 172,649 + -0.9 172,649 + +0.6 172,650 - -2.0 172,685 + -0.7 172,686 - -0.5 172,725 - -1.7 172,739 + +2.3 172,740 - +3.2 172,756 + -2.0 172,756 + -1.3 172,756 + +1.3 172,756 + +0.4 172,756 + +2.1 172,757 - +0.5 172,843 - BACOVA_04287 0.11 -2.8 172,856 - BACOVA_04287 0.12 +3.0 172,869 - BACOVA_04287 0.13 -2.9 172,894 - BACOVA_04287 0.16 -1.3 172,941 + BACOVA_04287 0.20 +0.1 172,942 - BACOVA_04287 0.21 -1.1 172,946 + BACOVA_04287 0.21 +0.3 172,947 - BACOVA_04287 0.21 -1.9 172,962 + BACOVA_04287 0.23 +3.5 172,962 + BACOVA_04287 0.23 -0.7 172,962 + BACOVA_04287 0.23 -2.2 172,963 - BACOVA_04287 0.23 +0.2 172,963 - BACOVA_04287 0.23 -0.4 172,965 - BACOVA_04287 0.23 -2.1 172,989 + BACOVA_04287 0.25 +1.2
Or see this region's nucleotide sequence