Experiment: Pullulan 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BACOVA_03064 and BACOVA_03065 are separated by 15 nucleotides BACOVA_03065 and BACOVA_03066 are separated by 50 nucleotides BACOVA_03066 and BACOVA_03067 are separated by 110 nucleotides
BACOVA_03064: BACOVA_03064 - DNA-binding helix-turn-helix protein, at 137,156 to 137,575
_03064
BACOVA_03065: BACOVA_03065 - DNA-binding helix-turn-helix protein, at 137,591 to 137,995
_03065
BACOVA_03066: BACOVA_03066 - hypothetical protein, at 138,046 to 138,171
_03066
BACOVA_03067: BACOVA_03067 - hypothetical protein, at 138,282 to 139,298
_03067
Position (kb) 138
139 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 137.065 kb on + strand at 137.069 kb on - strand at 137.092 kb on + strand at 137.093 kb on - strand at 137.093 kb on - strand at 137.093 kb on - strand at 137.119 kb on - strand at 137.138 kb on + strand at 137.138 kb on + strand at 137.155 kb on + strand at 137.162 kb on - strand at 137.162 kb on - strand at 137.165 kb on - strand at 137.226 kb on - strand, within BACOVA_03064 at 137.226 kb on - strand, within BACOVA_03064 at 137.226 kb on - strand, within BACOVA_03064 at 137.270 kb on - strand, within BACOVA_03064 at 137.270 kb on - strand, within BACOVA_03064 at 137.353 kb on - strand, within BACOVA_03064 at 137.353 kb on - strand, within BACOVA_03064 at 137.353 kb on - strand, within BACOVA_03064 at 137.407 kb on + strand, within BACOVA_03064 at 137.407 kb on + strand, within BACOVA_03064 at 137.412 kb on - strand, within BACOVA_03064 at 137.453 kb on + strand, within BACOVA_03064 at 137.453 kb on + strand, within BACOVA_03064 at 137.454 kb on - strand, within BACOVA_03064 at 137.465 kb on - strand, within BACOVA_03064 at 137.476 kb on + strand, within BACOVA_03064 at 137.477 kb on - strand, within BACOVA_03064 at 137.505 kb on - strand, within BACOVA_03064 at 137.548 kb on - strand at 137.548 kb on - strand at 137.573 kb on + strand at 137.614 kb on + strand at 137.614 kb on + strand at 137.614 kb on + strand at 137.614 kb on + strand at 137.615 kb on - strand at 137.696 kb on - strand, within BACOVA_03065 at 137.755 kb on - strand, within BACOVA_03065 at 137.787 kb on + strand, within BACOVA_03065 at 137.788 kb on - strand, within BACOVA_03065 at 137.825 kb on + strand, within BACOVA_03065 at 137.828 kb on - strand, within BACOVA_03065 at 137.828 kb on - strand, within BACOVA_03065 at 137.831 kb on - strand, within BACOVA_03065 at 137.831 kb on - strand, within BACOVA_03065 at 137.837 kb on - strand, within BACOVA_03065 at 137.875 kb on + strand, within BACOVA_03065 at 137.879 kb on - strand, within BACOVA_03065 at 137.879 kb on - strand, within BACOVA_03065 at 137.885 kb on - strand, within BACOVA_03065 at 137.900 kb on - strand, within BACOVA_03065 at 137.993 kb on + strand at 137.993 kb on + strand at 138.027 kb on + strand at 138.028 kb on - strand at 138.028 kb on - strand at 138.031 kb on + strand at 138.040 kb on - strand at 138.050 kb on + strand at 138.051 kb on - strand at 138.051 kb on - strand at 138.051 kb on - strand at 138.084 kb on - strand, within BACOVA_03066 at 138.170 kb on - strand at 138.170 kb on - strand at 138.184 kb on + strand at 138.185 kb on - strand at 138.228 kb on + strand at 138.232 kb on + strand at 138.233 kb on - strand at 138.270 kb on + strand at 138.270 kb on + strand at 138.271 kb on - strand at 138.271 kb on - strand at 138.280 kb on + strand at 138.283 kb on + strand at 138.290 kb on + strand at 138.290 kb on + strand at 138.291 kb on - strand at 138.294 kb on + strand at 138.350 kb on + strand at 138.355 kb on + strand at 138.356 kb on - strand at 138.373 kb on - strand at 138.380 kb on + strand at 138.380 kb on + strand at 138.380 kb on + strand at 138.381 kb on - strand at 138.381 kb on - strand at 138.381 kb on - strand at 138.443 kb on - strand, within BACOVA_03067 at 138.446 kb on + strand, within BACOVA_03067 at 138.446 kb on + strand, within BACOVA_03067 at 138.447 kb on - strand, within BACOVA_03067 at 138.449 kb on + strand, within BACOVA_03067 at 138.449 kb on + strand, within BACOVA_03067 at 138.453 kb on + strand, within BACOVA_03067 at 138.475 kb on - strand, within BACOVA_03067 at 138.486 kb on + strand, within BACOVA_03067 at 138.486 kb on + strand, within BACOVA_03067 at 138.487 kb on - strand, within BACOVA_03067 at 138.487 kb on - strand, within BACOVA_03067 at 138.487 kb on - strand, within BACOVA_03067 at 138.487 kb on - strand, within BACOVA_03067 at 138.495 kb on - strand, within BACOVA_03067 at 138.497 kb on - strand, within BACOVA_03067 at 138.497 kb on - strand, within BACOVA_03067 at 138.552 kb on + strand, within BACOVA_03067 at 138.573 kb on + strand, within BACOVA_03067 at 138.632 kb on + strand, within BACOVA_03067 at 138.635 kb on - strand, within BACOVA_03067 at 138.646 kb on - strand, within BACOVA_03067 at 138.654 kb on + strand, within BACOVA_03067 at 138.685 kb on + strand, within BACOVA_03067 at 138.686 kb on - strand, within BACOVA_03067 at 138.686 kb on - strand, within BACOVA_03067 at 138.686 kb on - strand, within BACOVA_03067 at 138.686 kb on - strand, within BACOVA_03067 at 138.701 kb on - strand, within BACOVA_03067 at 138.704 kb on - strand, within BACOVA_03067 at 138.704 kb on - strand, within BACOVA_03067 at 138.708 kb on + strand, within BACOVA_03067 at 138.715 kb on - strand, within BACOVA_03067 at 138.733 kb on - strand, within BACOVA_03067 at 138.773 kb on + strand, within BACOVA_03067 at 138.781 kb on + strand, within BACOVA_03067 at 138.788 kb on + strand, within BACOVA_03067 at 138.789 kb on - strand, within BACOVA_03067 at 138.803 kb on + strand, within BACOVA_03067 at 138.862 kb on + strand, within BACOVA_03067 at 138.904 kb on + strand, within BACOVA_03067 at 138.904 kb on + strand, within BACOVA_03067 at 138.905 kb on - strand, within BACOVA_03067 at 138.931 kb on - strand, within BACOVA_03067 at 138.935 kb on + strand, within BACOVA_03067 at 138.935 kb on + strand, within BACOVA_03067 at 138.936 kb on - strand, within BACOVA_03067 at 138.936 kb on - strand, within BACOVA_03067 at 138.987 kb on + strand, within BACOVA_03067 at 139.024 kb on + strand, within BACOVA_03067 at 139.029 kb on - strand, within BACOVA_03067 at 139.032 kb on + strand, within BACOVA_03067 at 139.032 kb on + strand, within BACOVA_03067 at 139.043 kb on + strand, within BACOVA_03067 at 139.053 kb on + strand, within BACOVA_03067 at 139.054 kb on - strand, within BACOVA_03067 at 139.054 kb on - strand, within BACOVA_03067 at 139.055 kb on + strand, within BACOVA_03067 at 139.055 kb on + strand, within BACOVA_03067 at 139.063 kb on + strand, within BACOVA_03067 at 139.101 kb on + strand, within BACOVA_03067 at 139.102 kb on - strand, within BACOVA_03067 at 139.116 kb on + strand, within BACOVA_03067 at 139.117 kb on - strand, within BACOVA_03067 at 139.117 kb on - strand, within BACOVA_03067 at 139.124 kb on - strand, within BACOVA_03067 at 139.129 kb on + strand, within BACOVA_03067 at 139.129 kb on + strand, within BACOVA_03067 at 139.129 kb on + strand, within BACOVA_03067 at 139.170 kb on - strand, within BACOVA_03067
Per-strain Table
Position Strand Gene LocusTag Fraction Pullulan 2.5 mg/mL (C) remove 137,065 + +1.0 137,069 - +1.0 137,092 + +1.1 137,093 - +1.7 137,093 - -3.0 137,093 - -3.2 137,119 - +0.6 137,138 + +0.6 137,138 + -1.8 137,155 + +2.0 137,162 - +1.9 137,162 - -1.1 137,165 - -1.4 137,226 - BACOVA_03064 0.17 -1.0 137,226 - BACOVA_03064 0.17 -0.6 137,226 - BACOVA_03064 0.17 -0.5 137,270 - BACOVA_03064 0.27 -1.1 137,270 - BACOVA_03064 0.27 -1.1 137,353 - BACOVA_03064 0.47 -1.1 137,353 - BACOVA_03064 0.47 +0.6 137,353 - BACOVA_03064 0.47 +1.1 137,407 + BACOVA_03064 0.60 +2.3 137,407 + BACOVA_03064 0.60 -2.3 137,412 - BACOVA_03064 0.61 -1.8 137,453 + BACOVA_03064 0.71 +1.1 137,453 + BACOVA_03064 0.71 +0.7 137,454 - BACOVA_03064 0.71 -1.1 137,465 - BACOVA_03064 0.74 +3.2 137,476 + BACOVA_03064 0.76 +1.1 137,477 - BACOVA_03064 0.76 -1.7 137,505 - BACOVA_03064 0.83 -1.1 137,548 - +3.6 137,548 - -0.9 137,573 + -1.8 137,614 + +0.1 137,614 + -0.2 137,614 + -2.5 137,614 + -0.3 137,615 - +4.8 137,696 - BACOVA_03065 0.26 -0.0 137,755 - BACOVA_03065 0.40 +0.8 137,787 + BACOVA_03065 0.48 -0.1 137,788 - BACOVA_03065 0.49 -2.0 137,825 + BACOVA_03065 0.58 -0.3 137,828 - BACOVA_03065 0.59 -1.0 137,828 - BACOVA_03065 0.59 +0.4 137,831 - BACOVA_03065 0.59 +0.2 137,831 - BACOVA_03065 0.59 -0.3 137,837 - BACOVA_03065 0.61 -0.0 137,875 + BACOVA_03065 0.70 -1.6 137,879 - BACOVA_03065 0.71 -1.6 137,879 - BACOVA_03065 0.71 +1.0 137,885 - BACOVA_03065 0.73 -3.5 137,900 - BACOVA_03065 0.76 -0.4 137,993 + +0.9 137,993 + +0.4 138,027 + -0.3 138,028 - -0.4 138,028 - -0.9 138,031 + +0.9 138,040 - -0.3 138,050 + -1.2 138,051 - +2.9 138,051 - -0.1 138,051 - -0.1 138,084 - BACOVA_03066 0.30 -0.6 138,170 - -0.4 138,170 - -0.3 138,184 + +0.4 138,185 - -1.7 138,228 + +0.8 138,232 + +0.3 138,233 - +0.5 138,270 + -1.1 138,270 + -1.2 138,271 - -2.1 138,271 - -0.0 138,280 + +0.2 138,283 + +0.7 138,290 + +0.9 138,290 + -1.9 138,291 - +0.2 138,294 + +0.9 138,350 + +0.9 138,355 + -0.4 138,356 - +2.1 138,373 - +1.9 138,380 + -0.6 138,380 + -1.3 138,380 + +1.7 138,381 - -0.2 138,381 - +1.5 138,381 - -2.0 138,443 - BACOVA_03067 0.16 +1.7 138,446 + BACOVA_03067 0.16 +0.1 138,446 + BACOVA_03067 0.16 -0.1 138,447 - BACOVA_03067 0.16 +1.6 138,449 + BACOVA_03067 0.16 -1.0 138,449 + BACOVA_03067 0.16 -1.4 138,453 + BACOVA_03067 0.17 +3.9 138,475 - BACOVA_03067 0.19 -0.4 138,486 + BACOVA_03067 0.20 +0.1 138,486 + BACOVA_03067 0.20 +1.8 138,487 - BACOVA_03067 0.20 +2.0 138,487 - BACOVA_03067 0.20 -1.8 138,487 - BACOVA_03067 0.20 +1.4 138,487 - BACOVA_03067 0.20 +0.3 138,495 - BACOVA_03067 0.21 +1.1 138,497 - BACOVA_03067 0.21 -2.1 138,497 - BACOVA_03067 0.21 +0.1 138,552 + BACOVA_03067 0.27 -1.1 138,573 + BACOVA_03067 0.29 -0.1 138,632 + BACOVA_03067 0.34 -0.5 138,635 - BACOVA_03067 0.35 +3.7 138,646 - BACOVA_03067 0.36 -1.1 138,654 + BACOVA_03067 0.37 -1.5 138,685 + BACOVA_03067 0.40 +1.5 138,686 - BACOVA_03067 0.40 -2.2 138,686 - BACOVA_03067 0.40 -0.8 138,686 - BACOVA_03067 0.40 -1.7 138,686 - BACOVA_03067 0.40 +0.6 138,701 - BACOVA_03067 0.41 -2.3 138,704 - BACOVA_03067 0.41 -0.5 138,704 - BACOVA_03067 0.41 -1.4 138,708 + BACOVA_03067 0.42 -0.4 138,715 - BACOVA_03067 0.43 +0.8 138,733 - BACOVA_03067 0.44 +0.9 138,773 + BACOVA_03067 0.48 -1.0 138,781 + BACOVA_03067 0.49 +5.0 138,788 + BACOVA_03067 0.50 -1.5 138,789 - BACOVA_03067 0.50 -1.2 138,803 + BACOVA_03067 0.51 -0.7 138,862 + BACOVA_03067 0.57 -1.4 138,904 + BACOVA_03067 0.61 +1.5 138,904 + BACOVA_03067 0.61 +0.6 138,905 - BACOVA_03067 0.61 -0.6 138,931 - BACOVA_03067 0.64 +1.0 138,935 + BACOVA_03067 0.64 -0.4 138,935 + BACOVA_03067 0.64 +0.5 138,936 - BACOVA_03067 0.64 +0.1 138,936 - BACOVA_03067 0.64 -1.1 138,987 + BACOVA_03067 0.69 -0.1 139,024 + BACOVA_03067 0.73 -0.8 139,029 - BACOVA_03067 0.73 -1.4 139,032 + BACOVA_03067 0.74 +0.4 139,032 + BACOVA_03067 0.74 +2.4 139,043 + BACOVA_03067 0.75 -1.6 139,053 + BACOVA_03067 0.76 -0.3 139,054 - BACOVA_03067 0.76 -0.1 139,054 - BACOVA_03067 0.76 -1.3 139,055 + BACOVA_03067 0.76 +1.0 139,055 + BACOVA_03067 0.76 +0.4 139,063 + BACOVA_03067 0.77 -1.2 139,101 + BACOVA_03067 0.81 +0.1 139,102 - BACOVA_03067 0.81 +0.9 139,116 + BACOVA_03067 0.82 +1.1 139,117 - BACOVA_03067 0.82 -1.0 139,117 - BACOVA_03067 0.82 +0.9 139,124 - BACOVA_03067 0.83 +1.8 139,129 + BACOVA_03067 0.83 +1.0 139,129 + BACOVA_03067 0.83 +3.1 139,129 + BACOVA_03067 0.83 +0.0 139,170 - BACOVA_03067 0.87 +1.3
Or see this region's nucleotide sequence