Experiment: Pullulan 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BACOVA_02045 and BACOVA_02046 are separated by 137 nucleotides BACOVA_02046 and BACOVA_02047 are separated by 510 nucleotides BACOVA_02047 and BACOVA_02048 overlap by 68 nucleotides BACOVA_02048 and BACOVA_02049 are separated by 58 nucleotides BACOVA_02049 and BACOVA_02050 are separated by 41 nucleotides
BACOVA_02045: BACOVA_02045 - TonB-linked outer membrane protein, SusC/RagA family, at 350,851 to 354,216
_02045
BACOVA_02046: BACOVA_02046 - hypothetical protein, at 354,354 to 354,488
_02046
BACOVA_02047: BACOVA_02047 - hypothetical protein, at 354,999 to 355,127
_02047
BACOVA_02048: BACOVA_02048 - hypothetical protein, at 355,060 to 355,140
_02048
BACOVA_02049: BACOVA_02049 - sugar-binding domain protein, at 355,199 to 356,317
_02049
BACOVA_02050: BACOVA_02050 - ribokinase, at 356,359 to 357,273
_02050
Position (kb) 355
356
357 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 354.217 kb on + strand at 354.218 kb on - strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.273 kb on + strand at 354.273 kb on + strand at 354.292 kb on + strand at 354.293 kb on - strand at 354.304 kb on + strand at 354.307 kb on + strand at 354.307 kb on + strand at 354.311 kb on + strand at 354.314 kb on + strand at 354.335 kb on + strand at 354.339 kb on - strand at 354.352 kb on + strand at 354.375 kb on - strand, within BACOVA_02046 at 354.404 kb on - strand, within BACOVA_02046 at 354.474 kb on + strand, within BACOVA_02046 at 354.474 kb on + strand, within BACOVA_02046 at 354.486 kb on + strand at 354.486 kb on + strand at 354.499 kb on - strand at 354.499 kb on - strand at 354.541 kb on + strand at 354.542 kb on - strand at 354.542 kb on - strand at 354.646 kb on - strand at 354.676 kb on + strand at 354.676 kb on + strand at 354.689 kb on + strand at 354.689 kb on + strand at 354.698 kb on + strand at 354.713 kb on + strand at 354.715 kb on + strand at 354.720 kb on - strand at 354.726 kb on + strand at 354.752 kb on - strand at 354.752 kb on - strand at 354.756 kb on - strand at 354.777 kb on + strand at 354.777 kb on + strand at 354.778 kb on - strand at 354.788 kb on - strand at 354.800 kb on - strand at 354.844 kb on + strand at 354.845 kb on - strand at 354.866 kb on + strand at 354.867 kb on - strand at 354.875 kb on - strand at 354.875 kb on - strand at 354.888 kb on + strand at 354.901 kb on - strand at 354.911 kb on - strand at 354.911 kb on - strand at 355.003 kb on + strand at 355.003 kb on + strand at 355.008 kb on - strand at 355.009 kb on + strand at 355.009 kb on + strand at 355.010 kb on - strand at 355.018 kb on - strand, within BACOVA_02047 at 355.020 kb on - strand, within BACOVA_02047 at 355.020 kb on - strand, within BACOVA_02047 at 355.049 kb on + strand, within BACOVA_02047 at 355.126 kb on - strand at 355.135 kb on + strand at 355.138 kb on + strand at 355.139 kb on - strand at 355.185 kb on + strand at 355.215 kb on - strand at 355.239 kb on - strand at 355.241 kb on - strand at 355.247 kb on - strand at 355.319 kb on - strand, within BACOVA_02049 at 355.356 kb on - strand, within BACOVA_02049 at 355.367 kb on + strand, within BACOVA_02049 at 355.368 kb on - strand, within BACOVA_02049 at 355.372 kb on - strand, within BACOVA_02049 at 355.381 kb on - strand, within BACOVA_02049 at 355.382 kb on + strand, within BACOVA_02049 at 355.382 kb on + strand, within BACOVA_02049 at 355.387 kb on + strand, within BACOVA_02049 at 355.411 kb on + strand, within BACOVA_02049 at 355.411 kb on + strand, within BACOVA_02049 at 355.411 kb on + strand, within BACOVA_02049 at 355.411 kb on + strand, within BACOVA_02049 at 355.412 kb on - strand, within BACOVA_02049 at 355.412 kb on - strand, within BACOVA_02049 at 355.432 kb on + strand, within BACOVA_02049 at 355.433 kb on - strand, within BACOVA_02049 at 355.442 kb on + strand, within BACOVA_02049 at 355.472 kb on - strand, within BACOVA_02049 at 355.479 kb on - strand, within BACOVA_02049 at 355.487 kb on + strand, within BACOVA_02049 at 355.504 kb on + strand, within BACOVA_02049 at 355.534 kb on + strand, within BACOVA_02049 at 355.536 kb on + strand, within BACOVA_02049 at 355.633 kb on + strand, within BACOVA_02049 at 355.634 kb on - strand, within BACOVA_02049 at 355.642 kb on + strand, within BACOVA_02049 at 355.664 kb on - strand, within BACOVA_02049 at 355.676 kb on - strand, within BACOVA_02049 at 355.678 kb on + strand, within BACOVA_02049 at 355.678 kb on + strand, within BACOVA_02049 at 355.691 kb on + strand, within BACOVA_02049 at 355.698 kb on - strand, within BACOVA_02049 at 355.714 kb on - strand, within BACOVA_02049 at 355.725 kb on + strand, within BACOVA_02049 at 355.726 kb on - strand, within BACOVA_02049 at 355.726 kb on - strand, within BACOVA_02049 at 355.726 kb on - strand, within BACOVA_02049 at 355.739 kb on + strand, within BACOVA_02049 at 355.739 kb on + strand, within BACOVA_02049 at 355.754 kb on - strand, within BACOVA_02049 at 355.795 kb on + strand, within BACOVA_02049 at 355.796 kb on - strand, within BACOVA_02049 at 355.799 kb on - strand, within BACOVA_02049 at 355.799 kb on - strand, within BACOVA_02049 at 355.801 kb on + strand, within BACOVA_02049 at 355.857 kb on - strand, within BACOVA_02049 at 355.862 kb on - strand, within BACOVA_02049 at 355.882 kb on + strand, within BACOVA_02049 at 355.883 kb on - strand, within BACOVA_02049 at 355.934 kb on - strand, within BACOVA_02049 at 355.934 kb on - strand, within BACOVA_02049 at 355.935 kb on + strand, within BACOVA_02049 at 355.936 kb on - strand, within BACOVA_02049 at 355.940 kb on - strand, within BACOVA_02049 at 355.940 kb on - strand, within BACOVA_02049 at 355.941 kb on + strand, within BACOVA_02049 at 355.942 kb on - strand, within BACOVA_02049 at 355.942 kb on - strand, within BACOVA_02049 at 355.973 kb on + strand, within BACOVA_02049 at 355.973 kb on + strand, within BACOVA_02049 at 355.973 kb on + strand, within BACOVA_02049 at 355.973 kb on + strand, within BACOVA_02049 at 355.974 kb on - strand, within BACOVA_02049 at 355.974 kb on - strand, within BACOVA_02049 at 355.974 kb on - strand, within BACOVA_02049 at 355.976 kb on - strand, within BACOVA_02049 at 355.976 kb on - strand, within BACOVA_02049 at 355.993 kb on + strand, within BACOVA_02049 at 355.997 kb on + strand, within BACOVA_02049 at 355.998 kb on - strand, within BACOVA_02049 at 355.998 kb on - strand, within BACOVA_02049 at 356.018 kb on + strand, within BACOVA_02049 at 356.018 kb on + strand, within BACOVA_02049 at 356.019 kb on - strand, within BACOVA_02049 at 356.020 kb on + strand, within BACOVA_02049 at 356.021 kb on - strand, within BACOVA_02049 at 356.023 kb on + strand, within BACOVA_02049 at 356.024 kb on - strand, within BACOVA_02049 at 356.026 kb on - strand, within BACOVA_02049 at 356.026 kb on - strand, within BACOVA_02049 at 356.026 kb on - strand, within BACOVA_02049 at 356.108 kb on - strand, within BACOVA_02049 at 356.126 kb on - strand, within BACOVA_02049 at 356.164 kb on + strand, within BACOVA_02049 at 356.193 kb on - strand, within BACOVA_02049 at 356.343 kb on - strand at 356.343 kb on - strand at 356.347 kb on - strand at 356.347 kb on - strand at 356.356 kb on + strand at 356.356 kb on + strand at 356.357 kb on - strand at 356.382 kb on - strand at 356.391 kb on + strand at 356.398 kb on - strand at 356.494 kb on - strand, within BACOVA_02050 at 356.504 kb on + strand, within BACOVA_02050 at 356.540 kb on + strand, within BACOVA_02050 at 356.544 kb on + strand, within BACOVA_02050 at 356.544 kb on + strand, within BACOVA_02050 at 356.544 kb on + strand, within BACOVA_02050 at 356.610 kb on + strand, within BACOVA_02050 at 356.610 kb on + strand, within BACOVA_02050 at 356.611 kb on - strand, within BACOVA_02050 at 356.614 kb on + strand, within BACOVA_02050 at 356.614 kb on + strand, within BACOVA_02050 at 356.619 kb on - strand, within BACOVA_02050 at 356.625 kb on + strand, within BACOVA_02050 at 356.649 kb on - strand, within BACOVA_02050 at 356.721 kb on + strand, within BACOVA_02050 at 356.721 kb on + strand, within BACOVA_02050 at 356.721 kb on + strand, within BACOVA_02050 at 356.775 kb on + strand, within BACOVA_02050 at 356.809 kb on + strand, within BACOVA_02050 at 356.809 kb on + strand, within BACOVA_02050 at 356.810 kb on - strand, within BACOVA_02050 at 356.810 kb on - strand, within BACOVA_02050 at 356.842 kb on - strand, within BACOVA_02050 at 356.849 kb on + strand, within BACOVA_02050 at 356.850 kb on - strand, within BACOVA_02050 at 356.852 kb on + strand, within BACOVA_02050 at 356.917 kb on - strand, within BACOVA_02050 at 356.996 kb on - strand, within BACOVA_02050 at 356.997 kb on + strand, within BACOVA_02050 at 357.015 kb on - strand, within BACOVA_02050 at 357.030 kb on + strand, within BACOVA_02050 at 357.031 kb on - strand, within BACOVA_02050 at 357.075 kb on + strand, within BACOVA_02050 at 357.098 kb on + strand, within BACOVA_02050 at 357.098 kb on + strand, within BACOVA_02050 at 357.129 kb on + strand, within BACOVA_02050 at 357.129 kb on + strand, within BACOVA_02050 at 357.130 kb on - strand, within BACOVA_02050 at 357.152 kb on + strand, within BACOVA_02050 at 357.152 kb on + strand, within BACOVA_02050 at 357.189 kb on + strand at 357.190 kb on - strand at 357.191 kb on + strand at 357.202 kb on - strand at 357.202 kb on - strand at 357.243 kb on - strand at 357.246 kb on + strand at 357.247 kb on - strand at 357.247 kb on - strand at 357.249 kb on - strand at 357.266 kb on + strand at 357.267 kb on - strand at 357.272 kb on - strand at 357.281 kb on + strand at 357.282 kb on - strand at 357.282 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pullulan 2.5 mg/mL (C) remove 354,217 + -0.1 354,218 - -1.1 354,228 + -0.4 354,228 + +1.0 354,228 + -0.7 354,229 - -0.0 354,229 - -0.1 354,273 + -2.5 354,273 + -1.0 354,292 + +0.8 354,293 - +3.3 354,304 + -0.2 354,307 + +0.6 354,307 + -0.7 354,311 + +2.3 354,314 + +0.6 354,335 + +0.1 354,339 - -2.0 354,352 + -0.9 354,375 - BACOVA_02046 0.16 -1.2 354,404 - BACOVA_02046 0.37 -0.4 354,474 + BACOVA_02046 0.89 +1.2 354,474 + BACOVA_02046 0.89 +3.5 354,486 + +0.5 354,486 + -0.5 354,499 - -1.2 354,499 - +1.1 354,541 + +0.6 354,542 - -1.0 354,542 - +0.7 354,646 - -0.6 354,676 + -1.1 354,676 + +3.8 354,689 + -0.7 354,689 + -1.3 354,698 + +1.9 354,713 + -1.2 354,715 + +1.2 354,720 - -1.9 354,726 + +0.9 354,752 - +1.2 354,752 - +4.1 354,756 - -0.9 354,777 + +0.6 354,777 + +2.9 354,778 - +1.1 354,788 - +1.4 354,800 - +0.6 354,844 + +0.6 354,845 - -0.0 354,866 + -1.6 354,867 - +0.9 354,875 - -0.7 354,875 - +0.9 354,888 + -0.8 354,901 - +0.2 354,911 - -0.0 354,911 - -1.9 355,003 + -1.2 355,003 + -1.6 355,008 - -1.3 355,009 + -1.3 355,009 + -1.5 355,010 - -1.8 355,018 - BACOVA_02047 0.15 +2.6 355,020 - BACOVA_02047 0.16 +1.8 355,020 - BACOVA_02047 0.16 -3.2 355,049 + BACOVA_02047 0.39 -0.0 355,126 - +0.4 355,135 + +0.6 355,138 + +2.0 355,139 - +0.7 355,185 + +2.6 355,215 - +1.0 355,239 - +1.8 355,241 - -1.4 355,247 - +1.1 355,319 - BACOVA_02049 0.11 -0.4 355,356 - BACOVA_02049 0.14 -0.8 355,367 + BACOVA_02049 0.15 +0.9 355,368 - BACOVA_02049 0.15 -2.2 355,372 - BACOVA_02049 0.15 -3.7 355,381 - BACOVA_02049 0.16 -0.1 355,382 + BACOVA_02049 0.16 +0.2 355,382 + BACOVA_02049 0.16 +0.7 355,387 + BACOVA_02049 0.17 +0.9 355,411 + BACOVA_02049 0.19 -0.1 355,411 + BACOVA_02049 0.19 -1.6 355,411 + BACOVA_02049 0.19 +0.8 355,411 + BACOVA_02049 0.19 +0.9 355,412 - BACOVA_02049 0.19 -0.4 355,412 - BACOVA_02049 0.19 +1.1 355,432 + BACOVA_02049 0.21 -2.5 355,433 - BACOVA_02049 0.21 -0.6 355,442 + BACOVA_02049 0.22 -0.5 355,472 - BACOVA_02049 0.24 -1.0 355,479 - BACOVA_02049 0.25 -1.8 355,487 + BACOVA_02049 0.26 +0.2 355,504 + BACOVA_02049 0.27 -2.1 355,534 + BACOVA_02049 0.30 -2.7 355,536 + BACOVA_02049 0.30 -0.7 355,633 + BACOVA_02049 0.39 -3.1 355,634 - BACOVA_02049 0.39 +1.6 355,642 + BACOVA_02049 0.40 -0.9 355,664 - BACOVA_02049 0.42 -0.2 355,676 - BACOVA_02049 0.43 +1.8 355,678 + BACOVA_02049 0.43 -2.5 355,678 + BACOVA_02049 0.43 +2.0 355,691 + BACOVA_02049 0.44 -2.4 355,698 - BACOVA_02049 0.45 -0.9 355,714 - BACOVA_02049 0.46 -2.9 355,725 + BACOVA_02049 0.47 +2.1 355,726 - BACOVA_02049 0.47 -0.8 355,726 - BACOVA_02049 0.47 +1.6 355,726 - BACOVA_02049 0.47 +1.0 355,739 + BACOVA_02049 0.48 -0.7 355,739 + BACOVA_02049 0.48 -0.8 355,754 - BACOVA_02049 0.50 -0.4 355,795 + BACOVA_02049 0.53 +1.0 355,796 - BACOVA_02049 0.53 -0.7 355,799 - BACOVA_02049 0.54 -0.3 355,799 - BACOVA_02049 0.54 +0.0 355,801 + BACOVA_02049 0.54 -3.2 355,857 - BACOVA_02049 0.59 +2.2 355,862 - BACOVA_02049 0.59 -3.2 355,882 + BACOVA_02049 0.61 -0.3 355,883 - BACOVA_02049 0.61 +0.2 355,934 - BACOVA_02049 0.66 +0.2 355,934 - BACOVA_02049 0.66 -1.8 355,935 + BACOVA_02049 0.66 -2.3 355,936 - BACOVA_02049 0.66 +2.3 355,940 - BACOVA_02049 0.66 -2.0 355,940 - BACOVA_02049 0.66 -0.8 355,941 + BACOVA_02049 0.66 -0.8 355,942 - BACOVA_02049 0.66 +3.0 355,942 - BACOVA_02049 0.66 -2.4 355,973 + BACOVA_02049 0.69 -1.4 355,973 + BACOVA_02049 0.69 +0.9 355,973 + BACOVA_02049 0.69 +0.2 355,973 + BACOVA_02049 0.69 -2.0 355,974 - BACOVA_02049 0.69 -0.3 355,974 - BACOVA_02049 0.69 +0.2 355,974 - BACOVA_02049 0.69 -0.5 355,976 - BACOVA_02049 0.69 -1.7 355,976 - BACOVA_02049 0.69 +2.6 355,993 + BACOVA_02049 0.71 -1.4 355,997 + BACOVA_02049 0.71 -1.0 355,998 - BACOVA_02049 0.71 +0.8 355,998 - BACOVA_02049 0.71 -1.6 356,018 + BACOVA_02049 0.73 -0.5 356,018 + BACOVA_02049 0.73 +2.8 356,019 - BACOVA_02049 0.73 -1.0 356,020 + BACOVA_02049 0.73 -1.5 356,021 - BACOVA_02049 0.73 -1.5 356,023 + BACOVA_02049 0.74 -2.5 356,024 - BACOVA_02049 0.74 -0.9 356,026 - BACOVA_02049 0.74 -0.5 356,026 - BACOVA_02049 0.74 -0.2 356,026 - BACOVA_02049 0.74 -2.6 356,108 - BACOVA_02049 0.81 -0.8 356,126 - BACOVA_02049 0.83 +0.1 356,164 + BACOVA_02049 0.86 -0.2 356,193 - BACOVA_02049 0.89 +2.6 356,343 - -0.3 356,343 - -0.1 356,347 - +1.1 356,347 - -0.8 356,356 + -0.1 356,356 + -1.0 356,357 - -2.1 356,382 - -2.5 356,391 + -0.3 356,398 - -1.5 356,494 - BACOVA_02050 0.15 +1.4 356,504 + BACOVA_02050 0.16 +1.1 356,540 + BACOVA_02050 0.20 +0.4 356,544 + BACOVA_02050 0.20 -1.3 356,544 + BACOVA_02050 0.20 +0.1 356,544 + BACOVA_02050 0.20 -1.1 356,610 + BACOVA_02050 0.27 -1.2 356,610 + BACOVA_02050 0.27 +0.6 356,611 - BACOVA_02050 0.28 +1.1 356,614 + BACOVA_02050 0.28 -0.2 356,614 + BACOVA_02050 0.28 +0.5 356,619 - BACOVA_02050 0.28 +2.6 356,625 + BACOVA_02050 0.29 +2.6 356,649 - BACOVA_02050 0.32 -2.2 356,721 + BACOVA_02050 0.40 +0.7 356,721 + BACOVA_02050 0.40 -0.7 356,721 + BACOVA_02050 0.40 +0.2 356,775 + BACOVA_02050 0.45 +1.5 356,809 + BACOVA_02050 0.49 +0.5 356,809 + BACOVA_02050 0.49 +1.5 356,810 - BACOVA_02050 0.49 +1.9 356,810 - BACOVA_02050 0.49 -1.1 356,842 - BACOVA_02050 0.53 -0.1 356,849 + BACOVA_02050 0.54 -2.2 356,850 - BACOVA_02050 0.54 +0.6 356,852 + BACOVA_02050 0.54 +1.0 356,917 - BACOVA_02050 0.61 +3.4 356,996 - BACOVA_02050 0.70 +0.4 356,997 + BACOVA_02050 0.70 +0.7 357,015 - BACOVA_02050 0.72 +1.6 357,030 + BACOVA_02050 0.73 -1.4 357,031 - BACOVA_02050 0.73 -1.0 357,075 + BACOVA_02050 0.78 -1.5 357,098 + BACOVA_02050 0.81 -0.7 357,098 + BACOVA_02050 0.81 -1.3 357,129 + BACOVA_02050 0.84 -2.3 357,129 + BACOVA_02050 0.84 -0.0 357,130 - BACOVA_02050 0.84 +2.0 357,152 + BACOVA_02050 0.87 -0.0 357,152 + BACOVA_02050 0.87 -2.4 357,189 + -3.1 357,190 - -1.6 357,191 + -0.2 357,202 - -1.1 357,202 - +0.6 357,243 - -2.3 357,246 + +0.2 357,247 - -1.2 357,247 - -2.9 357,249 - -2.2 357,266 + +1.0 357,267 - +3.5 357,272 - -0.2 357,281 + -2.2 357,282 - +0.8 357,282 - -2.0
Or see this region's nucleotide sequence