Experiment: Pullulan 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BACOVA_00540 and BACOVA_00541 are separated by 126 nucleotides BACOVA_00541 and BACOVA_00542 are separated by 143 nucleotides BACOVA_00542 and BACOVA_00543 are separated by 17 nucleotides BACOVA_00543 and BACOVA_00544 are separated by 191 nucleotides BACOVA_00544 and BACOVA_00545 overlap by 4 nucleotides BACOVA_00545 and BACOVA_00546 are separated by 352 nucleotides
BACOVA_00540: BACOVA_00540 - hypothetical protein, at 37,694 to 40,675
_00540
BACOVA_00541: BACOVA_00541 - hypothetical protein, at 40,802 to 40,903
_00541
BACOVA_00542: BACOVA_00542 - N-acetylmuramoyl-L-alanine amidase, at 41,047 to 41,547
_00542
BACOVA_00543: BACOVA_00543 - putative DNA-binding protein, at 41,565 to 42,080
_00543
BACOVA_00544: BACOVA_00544 - hypothetical protein, at 42,272 to 42,379
_00544
BACOVA_00545: BACOVA_00545 - hypothetical protein, at 42,376 to 42,621
_00545
BACOVA_00546: BACOVA_00546 - hypothetical protein, at 42,974 to 43,198
_00546
Position (kb) 41
42
43 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 40.576 kb on + strand at 40.576 kb on + strand at 40.617 kb on + strand at 40.621 kb on + strand at 40.621 kb on + strand at 40.621 kb on + strand at 40.622 kb on - strand at 40.624 kb on + strand at 40.624 kb on + strand at 40.634 kb on + strand at 40.661 kb on - strand at 40.661 kb on - strand at 40.661 kb on - strand at 40.664 kb on + strand at 40.678 kb on - strand at 40.777 kb on + strand at 40.778 kb on - strand at 40.960 kb on + strand at 40.964 kb on + strand at 40.970 kb on + strand at 41.158 kb on + strand, within BACOVA_00542 at 41.298 kb on + strand, within BACOVA_00542 at 41.387 kb on + strand, within BACOVA_00542 at 41.390 kb on + strand, within BACOVA_00542 at 41.514 kb on + strand at 41.514 kb on + strand at 41.566 kb on + strand at 41.789 kb on + strand, within BACOVA_00543 at 41.982 kb on + strand, within BACOVA_00543 at 42.208 kb on + strand at 42.275 kb on + strand at 42.309 kb on + strand, within BACOVA_00544 at 42.427 kb on + strand, within BACOVA_00545 at 42.452 kb on + strand, within BACOVA_00545 at 42.468 kb on - strand, within BACOVA_00545 at 42.478 kb on + strand, within BACOVA_00545 at 42.513 kb on + strand, within BACOVA_00545 at 42.524 kb on + strand, within BACOVA_00545 at 42.525 kb on - strand, within BACOVA_00545 at 42.571 kb on + strand, within BACOVA_00545 at 42.587 kb on + strand, within BACOVA_00545 at 42.645 kb on + strand at 42.651 kb on + strand at 42.670 kb on + strand at 42.672 kb on + strand at 42.687 kb on + strand at 42.687 kb on + strand at 42.691 kb on + strand at 42.691 kb on + strand at 42.713 kb on - strand at 42.730 kb on - strand at 42.734 kb on - strand at 42.736 kb on - strand at 42.769 kb on - strand at 42.769 kb on - strand at 42.813 kb on + strand at 42.878 kb on + strand at 42.905 kb on + strand at 42.905 kb on + strand at 42.909 kb on + strand at 42.909 kb on + strand at 42.934 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pullulan 2.5 mg/mL (C) remove 40,576 + +1.2 40,576 + -2.6 40,617 + -0.3 40,621 + -0.8 40,621 + +0.0 40,621 + +1.5 40,622 - -0.4 40,624 + +0.6 40,624 + +4.5 40,634 + -2.0 40,661 - -2.5 40,661 - +0.0 40,661 - +0.7 40,664 + -1.2 40,678 - +3.5 40,777 + -2.2 40,778 - +0.3 40,960 + -1.5 40,964 + +3.0 40,970 + -1.6 41,158 + BACOVA_00542 0.22 +0.7 41,298 + BACOVA_00542 0.50 -1.5 41,387 + BACOVA_00542 0.68 -0.1 41,390 + BACOVA_00542 0.68 +3.0 41,514 + -1.1 41,514 + -1.0 41,566 + -1.5 41,789 + BACOVA_00543 0.43 -1.1 41,982 + BACOVA_00543 0.81 -2.4 42,208 + +3.1 42,275 + -1.2 42,309 + BACOVA_00544 0.34 +0.3 42,427 + BACOVA_00545 0.21 +1.5 42,452 + BACOVA_00545 0.31 -2.7 42,468 - BACOVA_00545 0.37 +0.0 42,478 + BACOVA_00545 0.41 +0.9 42,513 + BACOVA_00545 0.56 -0.2 42,524 + BACOVA_00545 0.60 +1.0 42,525 - BACOVA_00545 0.61 +0.8 42,571 + BACOVA_00545 0.79 -1.2 42,587 + BACOVA_00545 0.86 +1.0 42,645 + -1.4 42,651 + -2.6 42,670 + +2.2 42,672 + +1.4 42,687 + -1.5 42,687 + -1.6 42,691 + +0.0 42,691 + +1.2 42,713 - +0.2 42,730 - -0.5 42,734 - -1.0 42,736 - +1.0 42,769 - -1.1 42,769 - -0.4 42,813 + -1.7 42,878 + +3.0 42,905 + -3.2 42,905 + -0.8 42,909 + -1.4 42,909 + -0.4 42,934 + -2.7
Or see this region's nucleotide sequence