Experiment: Pullulan 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BACOVA_00250 and BACOVA_00251 are separated by 109 nucleotides BACOVA_00251 and BACOVA_00252 are separated by 151 nucleotides BACOVA_00252 and BACOVA_00253 are separated by 53 nucleotides BACOVA_00253 and BACOVA_00254 are separated by 9 nucleotides
BACOVA_00250: BACOVA_00250 - glycosyl hydrolase family 2, sugar binding domain protein, at 33,245 to 35,605
_00250
BACOVA_00251: BACOVA_00251 - hypothetical protein, at 35,715 to 35,816
_00251
BACOVA_00252: BACOVA_00252 - hypothetical protein, at 35,968 to 36,168
_00252
BACOVA_00253: BACOVA_00253 - hypothetical protein, at 36,222 to 36,485
_00253
BACOVA_00254: BACOVA_00254 - hypothetical protein, at 36,495 to 36,836
_00254
Position (kb) 35
36
37 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 35.057 kb on - strand, within BACOVA_00250 at 35.086 kb on + strand, within BACOVA_00250 at 35.087 kb on - strand, within BACOVA_00250 at 35.087 kb on - strand, within BACOVA_00250 at 35.087 kb on - strand, within BACOVA_00250 at 35.129 kb on - strand, within BACOVA_00250 at 35.173 kb on + strand, within BACOVA_00250 at 35.173 kb on + strand, within BACOVA_00250 at 35.173 kb on + strand, within BACOVA_00250 at 35.174 kb on - strand, within BACOVA_00250 at 35.174 kb on - strand, within BACOVA_00250 at 35.178 kb on + strand, within BACOVA_00250 at 35.178 kb on + strand, within BACOVA_00250 at 35.179 kb on - strand, within BACOVA_00250 at 35.179 kb on - strand, within BACOVA_00250 at 35.187 kb on + strand, within BACOVA_00250 at 35.188 kb on - strand, within BACOVA_00250 at 35.188 kb on - strand, within BACOVA_00250 at 35.188 kb on - strand, within BACOVA_00250 at 35.193 kb on - strand, within BACOVA_00250 at 35.193 kb on - strand, within BACOVA_00250 at 35.195 kb on - strand, within BACOVA_00250 at 35.195 kb on - strand, within BACOVA_00250 at 35.211 kb on + strand, within BACOVA_00250 at 35.222 kb on + strand, within BACOVA_00250 at 35.258 kb on - strand, within BACOVA_00250 at 35.397 kb on + strand at 35.397 kb on + strand at 35.398 kb on - strand at 35.477 kb on - strand at 35.481 kb on + strand at 35.481 kb on + strand at 35.482 kb on - strand at 35.486 kb on - strand at 35.489 kb on + strand at 35.536 kb on + strand at 35.536 kb on + strand at 35.537 kb on - strand at 35.541 kb on + strand at 35.541 kb on + strand at 35.542 kb on - strand at 35.542 kb on - strand at 35.542 kb on - strand at 35.567 kb on - strand at 35.567 kb on - strand at 35.572 kb on + strand at 35.594 kb on - strand at 35.596 kb on + strand at 35.603 kb on + strand at 35.631 kb on + strand at 35.631 kb on + strand at 35.631 kb on + strand at 35.661 kb on - strand at 35.664 kb on + strand at 35.665 kb on - strand at 35.711 kb on + strand at 35.716 kb on + strand at 35.717 kb on - strand at 35.721 kb on - strand at 35.762 kb on + strand, within BACOVA_00251 at 35.762 kb on + strand, within BACOVA_00251 at 35.763 kb on - strand, within BACOVA_00251 at 35.817 kb on - strand at 35.895 kb on - strand at 35.924 kb on + strand at 35.974 kb on + strand at 36.038 kb on + strand, within BACOVA_00252 at 36.045 kb on + strand, within BACOVA_00252 at 36.046 kb on - strand, within BACOVA_00252 at 36.048 kb on + strand, within BACOVA_00252 at 36.049 kb on - strand, within BACOVA_00252 at 36.059 kb on + strand, within BACOVA_00252 at 36.060 kb on - strand, within BACOVA_00252 at 36.065 kb on - strand, within BACOVA_00252 at 36.079 kb on + strand, within BACOVA_00252 at 36.080 kb on - strand, within BACOVA_00252 at 36.087 kb on + strand, within BACOVA_00252 at 36.088 kb on - strand, within BACOVA_00252 at 36.118 kb on + strand, within BACOVA_00252 at 36.119 kb on - strand, within BACOVA_00252 at 36.131 kb on - strand, within BACOVA_00252 at 36.133 kb on + strand, within BACOVA_00252 at 36.133 kb on + strand, within BACOVA_00252 at 36.138 kb on + strand, within BACOVA_00252 at 36.154 kb on - strand at 36.182 kb on - strand at 36.184 kb on + strand at 36.185 kb on - strand at 36.190 kb on - strand at 36.197 kb on - strand at 36.205 kb on - strand at 36.215 kb on + strand at 36.228 kb on - strand at 36.241 kb on - strand at 36.245 kb on + strand at 36.246 kb on - strand at 36.249 kb on - strand, within BACOVA_00253 at 36.251 kb on + strand, within BACOVA_00253 at 36.251 kb on + strand, within BACOVA_00253 at 36.251 kb on + strand, within BACOVA_00253 at 36.251 kb on + strand, within BACOVA_00253 at 36.252 kb on - strand, within BACOVA_00253 at 36.252 kb on - strand, within BACOVA_00253 at 36.252 kb on - strand, within BACOVA_00253 at 36.280 kb on - strand, within BACOVA_00253 at 36.281 kb on + strand, within BACOVA_00253 at 36.352 kb on + strand, within BACOVA_00253 at 36.352 kb on + strand, within BACOVA_00253 at 36.452 kb on + strand, within BACOVA_00253 at 36.453 kb on - strand, within BACOVA_00253 at 36.466 kb on + strand at 36.466 kb on + strand at 36.467 kb on - strand at 36.483 kb on + strand at 36.494 kb on - strand at 36.564 kb on - strand, within BACOVA_00254 at 36.571 kb on + strand, within BACOVA_00254 at 36.575 kb on + strand, within BACOVA_00254 at 36.576 kb on - strand, within BACOVA_00254 at 36.576 kb on - strand, within BACOVA_00254 at 36.576 kb on - strand, within BACOVA_00254 at 36.589 kb on + strand, within BACOVA_00254 at 36.590 kb on - strand, within BACOVA_00254 at 36.605 kb on + strand, within BACOVA_00254 at 36.606 kb on - strand, within BACOVA_00254 at 36.606 kb on - strand, within BACOVA_00254 at 36.608 kb on + strand, within BACOVA_00254 at 36.628 kb on + strand, within BACOVA_00254 at 36.629 kb on - strand, within BACOVA_00254 at 36.629 kb on - strand, within BACOVA_00254 at 36.657 kb on - strand, within BACOVA_00254 at 36.671 kb on + strand, within BACOVA_00254 at 36.689 kb on + strand, within BACOVA_00254 at 36.689 kb on + strand, within BACOVA_00254 at 36.690 kb on - strand, within BACOVA_00254 at 36.692 kb on + strand, within BACOVA_00254 at 36.693 kb on - strand, within BACOVA_00254 at 36.698 kb on - strand, within BACOVA_00254 at 36.698 kb on - strand, within BACOVA_00254 at 36.725 kb on + strand, within BACOVA_00254 at 36.726 kb on - strand, within BACOVA_00254 at 36.726 kb on - strand, within BACOVA_00254 at 36.790 kb on + strand, within BACOVA_00254 at 36.802 kb on - strand at 36.805 kb on + strand at 36.806 kb on - strand at 36.815 kb on - strand at 36.820 kb on - strand at 36.820 kb on - strand at 36.855 kb on - strand at 36.858 kb on + strand at 36.865 kb on + strand at 36.880 kb on + strand at 36.893 kb on + strand at 36.904 kb on - strand at 36.915 kb on + strand at 36.915 kb on + strand at 36.918 kb on - strand at 36.918 kb on - strand at 36.925 kb on + strand at 37.057 kb on - strand at 37.078 kb on + strand at 37.098 kb on + strand at 37.098 kb on + strand at 37.098 kb on + strand at 37.099 kb on - strand at 37.104 kb on + strand at 37.145 kb on + strand at 37.146 kb on - strand at 37.153 kb on + strand at 37.154 kb on - strand at 37.165 kb on + strand at 37.166 kb on - strand at 37.166 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pullulan 2.5 mg/mL (C) remove 35,057 - BACOVA_00250 0.77 -0.8 35,086 + BACOVA_00250 0.78 +0.4 35,087 - BACOVA_00250 0.78 +1.2 35,087 - BACOVA_00250 0.78 -1.5 35,087 - BACOVA_00250 0.78 -2.0 35,129 - BACOVA_00250 0.80 -0.6 35,173 + BACOVA_00250 0.82 -1.1 35,173 + BACOVA_00250 0.82 +0.3 35,173 + BACOVA_00250 0.82 +2.5 35,174 - BACOVA_00250 0.82 +1.1 35,174 - BACOVA_00250 0.82 +0.9 35,178 + BACOVA_00250 0.82 -0.4 35,178 + BACOVA_00250 0.82 -0.8 35,179 - BACOVA_00250 0.82 -1.7 35,179 - BACOVA_00250 0.82 +1.3 35,187 + BACOVA_00250 0.82 -0.2 35,188 - BACOVA_00250 0.82 -0.1 35,188 - BACOVA_00250 0.82 -0.3 35,188 - BACOVA_00250 0.82 +1.3 35,193 - BACOVA_00250 0.83 +1.0 35,193 - BACOVA_00250 0.83 +3.1 35,195 - BACOVA_00250 0.83 -1.8 35,195 - BACOVA_00250 0.83 +0.5 35,211 + BACOVA_00250 0.83 -0.8 35,222 + BACOVA_00250 0.84 -0.6 35,258 - BACOVA_00250 0.85 -0.9 35,397 + +1.5 35,397 + +1.9 35,398 - -1.7 35,477 - -1.6 35,481 + +0.2 35,481 + -0.7 35,482 - +1.1 35,486 - +2.4 35,489 + -0.1 35,536 + -1.9 35,536 + +1.9 35,537 - +1.3 35,541 + +0.6 35,541 + -0.1 35,542 - -1.4 35,542 - -0.9 35,542 - +0.5 35,567 - +0.0 35,567 - -0.7 35,572 + +0.8 35,594 - -1.3 35,596 + -3.3 35,603 + -0.6 35,631 + +0.6 35,631 + +1.2 35,631 + -1.1 35,661 - -0.8 35,664 + -0.9 35,665 - +2.1 35,711 + +1.9 35,716 + -0.4 35,717 - +0.7 35,721 - +3.6 35,762 + BACOVA_00251 0.46 +1.4 35,762 + BACOVA_00251 0.46 -0.3 35,763 - BACOVA_00251 0.47 -0.4 35,817 - +1.7 35,895 - +2.0 35,924 + -1.8 35,974 + -1.9 36,038 + BACOVA_00252 0.35 -0.7 36,045 + BACOVA_00252 0.38 -1.8 36,046 - BACOVA_00252 0.39 +1.1 36,048 + BACOVA_00252 0.40 -1.8 36,049 - BACOVA_00252 0.40 +1.2 36,059 + BACOVA_00252 0.45 +1.6 36,060 - BACOVA_00252 0.46 +0.1 36,065 - BACOVA_00252 0.48 -1.6 36,079 + BACOVA_00252 0.55 +0.5 36,080 - BACOVA_00252 0.56 +3.2 36,087 + BACOVA_00252 0.59 -0.4 36,088 - BACOVA_00252 0.60 +1.6 36,118 + BACOVA_00252 0.75 -0.1 36,119 - BACOVA_00252 0.75 -1.5 36,131 - BACOVA_00252 0.81 +0.1 36,133 + BACOVA_00252 0.82 -2.1 36,133 + BACOVA_00252 0.82 -1.0 36,138 + BACOVA_00252 0.85 -2.0 36,154 - -0.0 36,182 - -1.8 36,184 + -2.8 36,185 - -0.9 36,190 - -1.7 36,197 - -1.4 36,205 - +0.6 36,215 + -0.5 36,228 - +0.7 36,241 - -2.5 36,245 + -0.9 36,246 - -1.2 36,249 - BACOVA_00253 0.10 +0.5 36,251 + BACOVA_00253 0.11 +0.5 36,251 + BACOVA_00253 0.11 +3.4 36,251 + BACOVA_00253 0.11 +1.0 36,251 + BACOVA_00253 0.11 +1.4 36,252 - BACOVA_00253 0.11 -1.9 36,252 - BACOVA_00253 0.11 +1.1 36,252 - BACOVA_00253 0.11 -2.4 36,280 - BACOVA_00253 0.22 -1.0 36,281 + BACOVA_00253 0.22 -1.3 36,352 + BACOVA_00253 0.49 +0.8 36,352 + BACOVA_00253 0.49 -0.2 36,452 + BACOVA_00253 0.87 +0.2 36,453 - BACOVA_00253 0.88 -3.1 36,466 + +3.7 36,466 + -2.9 36,467 - -1.9 36,483 + +0.2 36,494 - +0.4 36,564 - BACOVA_00254 0.20 -1.5 36,571 + BACOVA_00254 0.22 +1.2 36,575 + BACOVA_00254 0.23 +0.6 36,576 - BACOVA_00254 0.24 -0.5 36,576 - BACOVA_00254 0.24 +0.2 36,576 - BACOVA_00254 0.24 -1.4 36,589 + BACOVA_00254 0.27 +0.8 36,590 - BACOVA_00254 0.28 -2.7 36,605 + BACOVA_00254 0.32 -0.0 36,606 - BACOVA_00254 0.32 +0.9 36,606 - BACOVA_00254 0.32 -0.5 36,608 + BACOVA_00254 0.33 +1.6 36,628 + BACOVA_00254 0.39 -3.0 36,629 - BACOVA_00254 0.39 -2.8 36,629 - BACOVA_00254 0.39 -2.3 36,657 - BACOVA_00254 0.47 +1.5 36,671 + BACOVA_00254 0.51 +2.4 36,689 + BACOVA_00254 0.57 -1.6 36,689 + BACOVA_00254 0.57 +1.0 36,690 - BACOVA_00254 0.57 -0.8 36,692 + BACOVA_00254 0.58 +1.3 36,693 - BACOVA_00254 0.58 +0.1 36,698 - BACOVA_00254 0.59 -1.1 36,698 - BACOVA_00254 0.59 +0.4 36,725 + BACOVA_00254 0.67 +2.6 36,726 - BACOVA_00254 0.68 +0.3 36,726 - BACOVA_00254 0.68 +0.3 36,790 + BACOVA_00254 0.86 -1.1 36,802 - +1.8 36,805 + -0.9 36,806 - -1.5 36,815 - +0.3 36,820 - -0.1 36,820 - -0.2 36,855 - -2.7 36,858 + +1.5 36,865 + +0.5 36,880 + -1.6 36,893 + +1.8 36,904 - +1.0 36,915 + +0.0 36,915 + -0.2 36,918 - +2.1 36,918 - -0.4 36,925 + -0.4 37,057 - +3.3 37,078 + -0.8 37,098 + +1.5 37,098 + -0.9 37,098 + -0.4 37,099 - +0.1 37,104 + -0.8 37,145 + +0.0 37,146 - -1.5 37,153 + +0.6 37,154 - +1.3 37,165 + -1.3 37,166 - -1.6 37,166 - +0.3
Or see this region's nucleotide sequence