Strain Fitness in Escherichia coli HS(pFamp)R (ATCC 700891) around OHPLBJKB_00708

Experiment: T3 MOI10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOHPLBJKB_00705 and OHPLBJKB_00706 overlap by 1 nucleotidesOHPLBJKB_00706 and OHPLBJKB_00707 overlap by 8 nucleotidesOHPLBJKB_00707 and OHPLBJKB_00708 overlap by 8 nucleotidesOHPLBJKB_00708 and OHPLBJKB_00709 are separated by 12 nucleotidesOHPLBJKB_00709 and OHPLBJKB_00710 are separated by 122 nucleotides OHPLBJKB_00705: OHPLBJKB_00705 - Hydrogenase-2 large chain, at 723,082 to 724,785 _00705 OHPLBJKB_00706: OHPLBJKB_00706 - Hydrogenase 2 maturation protease, at 724,785 to 725,279 _00706 OHPLBJKB_00707: OHPLBJKB_00707 - Hydrogenase-2 operon protein HybE, at 725,272 to 725,760 _00707 OHPLBJKB_00708: OHPLBJKB_00708 - Hydrogenase maturation factor HybF, at 725,753 to 726,094 _00708 OHPLBJKB_00709: OHPLBJKB_00709 - Hydrogenase maturation factor HybG, at 726,107 to 726,355 _00709 OHPLBJKB_00710: OHPLBJKB_00710 - Disulfide-bond oxidoreductase YghU, at 726,478 to 727,344 _00710 Position (kb) 725 726 727Strain fitness (log2 ratio) -2 -1 0 1 2at 724.790 kb on + strandat 724.791 kb on - strandat 724.791 kb on - strandat 724.791 kb on - strandat 724.795 kb on + strandat 724.801 kb on + strandat 724.802 kb on - strandat 724.872 kb on + strand, within OHPLBJKB_00706at 724.872 kb on + strand, within OHPLBJKB_00706at 724.872 kb on + strand, within OHPLBJKB_00706at 724.873 kb on - strand, within OHPLBJKB_00706at 724.887 kb on + strand, within OHPLBJKB_00706at 724.887 kb on + strand, within OHPLBJKB_00706at 724.888 kb on - strand, within OHPLBJKB_00706at 725.318 kb on + strandat 725.393 kb on - strand, within OHPLBJKB_00707at 725.393 kb on - strand, within OHPLBJKB_00707at 725.407 kb on - strand, within OHPLBJKB_00707at 725.544 kb on + strand, within OHPLBJKB_00707at 725.544 kb on + strand, within OHPLBJKB_00707at 725.585 kb on - strand, within OHPLBJKB_00707at 725.702 kb on + strand, within OHPLBJKB_00707at 725.702 kb on + strand, within OHPLBJKB_00707at 725.821 kb on + strand, within OHPLBJKB_00708at 725.822 kb on - strand, within OHPLBJKB_00708at 725.937 kb on + strand, within OHPLBJKB_00708at 725.937 kb on + strand, within OHPLBJKB_00708at 725.938 kb on - strand, within OHPLBJKB_00708at 725.938 kb on - strand, within OHPLBJKB_00708at 725.938 kb on - strand, within OHPLBJKB_00708at 725.938 kb on - strand, within OHPLBJKB_00708at 725.938 kb on - strand, within OHPLBJKB_00708at 725.941 kb on + strand, within OHPLBJKB_00708at 725.942 kb on - strand, within OHPLBJKB_00708at 725.942 kb on - strand, within OHPLBJKB_00708at 725.948 kb on + strand, within OHPLBJKB_00708at 726.105 kb on + strandat 726.105 kb on + strandat 726.105 kb on + strandat 726.105 kb on + strandat 726.106 kb on - strandat 726.113 kb on - strandat 726.152 kb on - strand, within OHPLBJKB_00709at 726.152 kb on - strand, within OHPLBJKB_00709at 726.177 kb on + strand, within OHPLBJKB_00709at 726.177 kb on + strand, within OHPLBJKB_00709at 726.177 kb on + strand, within OHPLBJKB_00709at 726.177 kb on + strand, within OHPLBJKB_00709at 726.177 kb on + strand, within OHPLBJKB_00709at 726.177 kb on + strand, within OHPLBJKB_00709at 726.177 kb on + strand, within OHPLBJKB_00709at 726.177 kb on + strand, within OHPLBJKB_00709at 726.177 kb on + strand, within OHPLBJKB_00709at 726.178 kb on - strand, within OHPLBJKB_00709at 726.178 kb on - strand, within OHPLBJKB_00709at 726.178 kb on - strand, within OHPLBJKB_00709at 726.178 kb on - strand, within OHPLBJKB_00709at 726.178 kb on - strand, within OHPLBJKB_00709at 726.178 kb on - strand, within OHPLBJKB_00709at 726.185 kb on - strand, within OHPLBJKB_00709at 726.185 kb on - strand, within OHPLBJKB_00709at 726.185 kb on - strand, within OHPLBJKB_00709at 726.238 kb on - strand, within OHPLBJKB_00709at 726.312 kb on + strand, within OHPLBJKB_00709at 726.313 kb on - strand, within OHPLBJKB_00709at 726.313 kb on - strand, within OHPLBJKB_00709at 726.336 kb on - strandat 726.360 kb on + strandat 726.360 kb on + strandat 726.361 kb on - strandat 726.361 kb on - strandat 726.370 kb on - strandat 726.370 kb on - strandat 726.378 kb on + strandat 726.492 kb on + strandat 726.576 kb on + strand, within OHPLBJKB_00710at 726.576 kb on + strand, within OHPLBJKB_00710at 726.608 kb on - strand, within OHPLBJKB_00710at 726.628 kb on + strand, within OHPLBJKB_00710at 726.628 kb on + strand, within OHPLBJKB_00710at 726.628 kb on + strand, within OHPLBJKB_00710at 726.628 kb on + strand, within OHPLBJKB_00710at 726.629 kb on - strand, within OHPLBJKB_00710at 726.629 kb on - strand, within OHPLBJKB_00710at 726.629 kb on - strand, within OHPLBJKB_00710at 726.629 kb on - strand, within OHPLBJKB_00710at 726.629 kb on - strand, within OHPLBJKB_00710at 726.636 kb on + strand, within OHPLBJKB_00710at 726.636 kb on + strand, within OHPLBJKB_00710at 726.636 kb on + strand, within OHPLBJKB_00710at 726.636 kb on + strand, within OHPLBJKB_00710at 726.636 kb on + strand, within OHPLBJKB_00710at 726.636 kb on + strand, within OHPLBJKB_00710at 726.636 kb on + strand, within OHPLBJKB_00710at 726.637 kb on - strand, within OHPLBJKB_00710at 726.638 kb on + strand, within OHPLBJKB_00710at 726.639 kb on - strand, within OHPLBJKB_00710at 726.639 kb on - strand, within OHPLBJKB_00710at 726.639 kb on - strand, within OHPLBJKB_00710at 726.639 kb on - strand, within OHPLBJKB_00710at 726.671 kb on + strand, within OHPLBJKB_00710at 726.671 kb on + strand, within OHPLBJKB_00710at 726.671 kb on + strand, within OHPLBJKB_00710at 726.672 kb on - strand, within OHPLBJKB_00710at 726.672 kb on - strand, within OHPLBJKB_00710at 726.682 kb on + strand, within OHPLBJKB_00710at 726.683 kb on - strand, within OHPLBJKB_00710at 726.683 kb on - strand, within OHPLBJKB_00710at 726.683 kb on - strand, within OHPLBJKB_00710at 726.731 kb on + strand, within OHPLBJKB_00710at 726.732 kb on - strand, within OHPLBJKB_00710at 726.753 kb on + strand, within OHPLBJKB_00710at 726.753 kb on + strand, within OHPLBJKB_00710at 726.753 kb on + strand, within OHPLBJKB_00710at 726.753 kb on + strand, within OHPLBJKB_00710at 726.753 kb on + strand, within OHPLBJKB_00710at 726.754 kb on - strand, within OHPLBJKB_00710at 726.754 kb on - strand, within OHPLBJKB_00710at 726.754 kb on - strand, within OHPLBJKB_00710at 726.754 kb on - strand, within OHPLBJKB_00710at 726.754 kb on - strand, within OHPLBJKB_00710at 726.754 kb on - strand, within OHPLBJKB_00710at 726.807 kb on + strand, within OHPLBJKB_00710at 726.808 kb on - strand, within OHPLBJKB_00710at 726.825 kb on - strand, within OHPLBJKB_00710at 726.836 kb on - strand, within OHPLBJKB_00710at 726.845 kb on + strand, within OHPLBJKB_00710at 726.851 kb on + strand, within OHPLBJKB_00710at 726.851 kb on + strand, within OHPLBJKB_00710at 726.975 kb on - strand, within OHPLBJKB_00710at 727.018 kb on - strand, within OHPLBJKB_00710at 727.023 kb on + strand, within OHPLBJKB_00710at 727.023 kb on + strand, within OHPLBJKB_00710at 727.023 kb on + strand, within OHPLBJKB_00710at 727.024 kb on - strand, within OHPLBJKB_00710at 727.024 kb on - strand, within OHPLBJKB_00710

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Per-strain Table

Position Strand Gene LocusTag Fraction T3 MOI10
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724,790 + -0.1
724,791 - +0.0
724,791 - -1.2
724,791 - +0.3
724,795 + +0.7
724,801 + -1.9
724,802 - +0.8
724,872 + OHPLBJKB_00706 0.18 -0.9
724,872 + OHPLBJKB_00706 0.18 +0.0
724,872 + OHPLBJKB_00706 0.18 -0.2
724,873 - OHPLBJKB_00706 0.18 -0.4
724,887 + OHPLBJKB_00706 0.21 -0.8
724,887 + OHPLBJKB_00706 0.21 -0.3
724,888 - OHPLBJKB_00706 0.21 +0.7
725,318 + -0.1
725,393 - OHPLBJKB_00707 0.25 +0.9
725,393 - OHPLBJKB_00707 0.25 +0.1
725,407 - OHPLBJKB_00707 0.28 +0.1
725,544 + OHPLBJKB_00707 0.56 +1.0
725,544 + OHPLBJKB_00707 0.56 -0.9
725,585 - OHPLBJKB_00707 0.64 +1.0
725,702 + OHPLBJKB_00707 0.88 -1.1
725,702 + OHPLBJKB_00707 0.88 -0.3
725,821 + OHPLBJKB_00708 0.20 -0.8
725,822 - OHPLBJKB_00708 0.20 -0.9
725,937 + OHPLBJKB_00708 0.54 -0.4
725,937 + OHPLBJKB_00708 0.54 -0.7
725,938 - OHPLBJKB_00708 0.54 -0.0
725,938 - OHPLBJKB_00708 0.54 +0.3
725,938 - OHPLBJKB_00708 0.54 -0.1
725,938 - OHPLBJKB_00708 0.54 -0.6
725,938 - OHPLBJKB_00708 0.54 -0.8
725,941 + OHPLBJKB_00708 0.55 -0.2
725,942 - OHPLBJKB_00708 0.55 +0.4
725,942 - OHPLBJKB_00708 0.55 +0.0
725,948 + OHPLBJKB_00708 0.57 -0.0
726,105 + -0.3
726,105 + +1.6
726,105 + -0.5
726,105 + -0.6
726,106 - +0.7
726,113 - -0.2
726,152 - OHPLBJKB_00709 0.18 +0.8
726,152 - OHPLBJKB_00709 0.18 +0.5
726,177 + OHPLBJKB_00709 0.28 +0.1
726,177 + OHPLBJKB_00709 0.28 +0.7
726,177 + OHPLBJKB_00709 0.28 -1.7
726,177 + OHPLBJKB_00709 0.28 -0.5
726,177 + OHPLBJKB_00709 0.28 -1.8
726,177 + OHPLBJKB_00709 0.28 +0.5
726,177 + OHPLBJKB_00709 0.28 +0.0
726,177 + OHPLBJKB_00709 0.28 -0.3
726,177 + OHPLBJKB_00709 0.28 +0.4
726,178 - OHPLBJKB_00709 0.29 -0.5
726,178 - OHPLBJKB_00709 0.29 -0.5
726,178 - OHPLBJKB_00709 0.29 -0.2
726,178 - OHPLBJKB_00709 0.29 +0.8
726,178 - OHPLBJKB_00709 0.29 +0.6
726,178 - OHPLBJKB_00709 0.29 +1.0
726,185 - OHPLBJKB_00709 0.31 -0.7
726,185 - OHPLBJKB_00709 0.31 +0.6
726,185 - OHPLBJKB_00709 0.31 +0.2
726,238 - OHPLBJKB_00709 0.53 +0.2
726,312 + OHPLBJKB_00709 0.82 -0.9
726,313 - OHPLBJKB_00709 0.83 +0.7
726,313 - OHPLBJKB_00709 0.83 -0.3
726,336 - -1.1
726,360 + -0.9
726,360 + -1.2
726,361 - -0.4
726,361 - +0.1
726,370 - -0.0
726,370 - -1.2
726,378 + +0.1
726,492 + +0.7
726,576 + OHPLBJKB_00710 0.11 +0.6
726,576 + OHPLBJKB_00710 0.11 -0.2
726,608 - OHPLBJKB_00710 0.15 -0.5
726,628 + OHPLBJKB_00710 0.17 +0.3
726,628 + OHPLBJKB_00710 0.17 -0.3
726,628 + OHPLBJKB_00710 0.17 -0.3
726,628 + OHPLBJKB_00710 0.17 +0.1
726,629 - OHPLBJKB_00710 0.17 +0.1
726,629 - OHPLBJKB_00710 0.17 +1.8
726,629 - OHPLBJKB_00710 0.17 -0.7
726,629 - OHPLBJKB_00710 0.17 -0.6
726,629 - OHPLBJKB_00710 0.17 -0.2
726,636 + OHPLBJKB_00710 0.18 -1.1
726,636 + OHPLBJKB_00710 0.18 -0.4
726,636 + OHPLBJKB_00710 0.18 +0.2
726,636 + OHPLBJKB_00710 0.18 -0.0
726,636 + OHPLBJKB_00710 0.18 -0.4
726,636 + OHPLBJKB_00710 0.18 -0.5
726,636 + OHPLBJKB_00710 0.18 -0.4
726,637 - OHPLBJKB_00710 0.18 +0.6
726,638 + OHPLBJKB_00710 0.18 +0.7
726,639 - OHPLBJKB_00710 0.19 -1.0
726,639 - OHPLBJKB_00710 0.19 +1.0
726,639 - OHPLBJKB_00710 0.19 +1.9
726,639 - OHPLBJKB_00710 0.19 -0.0
726,671 + OHPLBJKB_00710 0.22 -0.3
726,671 + OHPLBJKB_00710 0.22 +0.4
726,671 + OHPLBJKB_00710 0.22 -0.5
726,672 - OHPLBJKB_00710 0.22 -0.1
726,672 - OHPLBJKB_00710 0.22 -1.0
726,682 + OHPLBJKB_00710 0.24 -0.2
726,683 - OHPLBJKB_00710 0.24 -0.7
726,683 - OHPLBJKB_00710 0.24 +1.5
726,683 - OHPLBJKB_00710 0.24 -0.3
726,731 + OHPLBJKB_00710 0.29 -1.1
726,732 - OHPLBJKB_00710 0.29 +0.3
726,753 + OHPLBJKB_00710 0.32 -0.6
726,753 + OHPLBJKB_00710 0.32 +0.1
726,753 + OHPLBJKB_00710 0.32 -0.1
726,753 + OHPLBJKB_00710 0.32 +0.7
726,753 + OHPLBJKB_00710 0.32 +0.2
726,754 - OHPLBJKB_00710 0.32 +0.3
726,754 - OHPLBJKB_00710 0.32 +1.3
726,754 - OHPLBJKB_00710 0.32 +0.6
726,754 - OHPLBJKB_00710 0.32 -0.6
726,754 - OHPLBJKB_00710 0.32 -0.1
726,754 - OHPLBJKB_00710 0.32 +0.3
726,807 + OHPLBJKB_00710 0.38 +0.7
726,808 - OHPLBJKB_00710 0.38 -0.2
726,825 - OHPLBJKB_00710 0.40 +0.6
726,836 - OHPLBJKB_00710 0.41 +0.4
726,845 + OHPLBJKB_00710 0.42 -0.5
726,851 + OHPLBJKB_00710 0.43 -0.2
726,851 + OHPLBJKB_00710 0.43 -0.2
726,975 - OHPLBJKB_00710 0.57 -0.5
727,018 - OHPLBJKB_00710 0.62 +0.3
727,023 + OHPLBJKB_00710 0.63 +0.1
727,023 + OHPLBJKB_00710 0.63 -0.0
727,023 + OHPLBJKB_00710 0.63 -0.2
727,024 - OHPLBJKB_00710 0.63 +0.4
727,024 - OHPLBJKB_00710 0.63 -0.3

Or see this region's nucleotide sequence