Experiment: R2A_PIPES with Cobalt chloride 1024 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_12580 and dapB are separated by 147 nucleotides dapB and lpdA are separated by 46 nucleotides lpdA and aceF are separated by 15 nucleotides
OKGIIK_12580: OKGIIK_12580 - hypothetical protein, at 2,709,396 to 2,709,875
_12580
OKGIIK_12585: dapB - 4-hydroxy-tetrahydrodipicolinate reductase, at 2,710,023 to 2,710,823
dapB
OKGIIK_12590: lpdA - dihydrolipoyl dehydrogenase, at 2,710,870 to 2,712,654
lpdA
OKGIIK_12595: aceF - dihydrolipoyllysine-residue acetyltransferase, at 2,712,670 to 2,714,079
aceF
Position (kb)
2710
2711
2712
2713 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2709.879 kb on - strand at 2710.024 kb on + strand at 2710.024 kb on + strand at 2710.024 kb on + strand at 2710.024 kb on + strand at 2710.024 kb on + strand at 2710.024 kb on + strand at 2710.024 kb on + strand at 2710.025 kb on - strand at 2710.025 kb on - strand at 2710.025 kb on - strand at 2710.025 kb on - strand at 2710.025 kb on - strand at 2710.108 kb on + strand, within dapB at 2710.108 kb on + strand, within dapB at 2710.108 kb on + strand, within dapB at 2710.108 kb on + strand, within dapB at 2710.109 kb on - strand, within dapB at 2710.188 kb on + strand, within dapB at 2710.188 kb on + strand, within dapB at 2710.188 kb on + strand, within dapB at 2710.189 kb on - strand, within dapB at 2710.189 kb on - strand, within dapB at 2710.329 kb on + strand, within dapB at 2710.330 kb on - strand, within dapB at 2710.330 kb on - strand, within dapB at 2710.330 kb on - strand, within dapB at 2710.330 kb on - strand, within dapB at 2710.330 kb on - strand, within dapB at 2710.330 kb on - strand, within dapB at 2710.363 kb on + strand, within dapB at 2710.369 kb on + strand, within dapB at 2710.369 kb on + strand, within dapB at 2710.369 kb on + strand, within dapB at 2710.370 kb on - strand, within dapB at 2710.370 kb on - strand, within dapB at 2710.370 kb on - strand, within dapB at 2710.370 kb on - strand, within dapB at 2710.370 kb on - strand, within dapB at 2710.370 kb on - strand, within dapB at 2710.370 kb on - strand, within dapB at 2710.370 kb on - strand, within dapB at 2710.507 kb on + strand, within dapB at 2710.507 kb on + strand, within dapB at 2710.508 kb on - strand, within dapB at 2710.508 kb on - strand, within dapB at 2710.508 kb on - strand, within dapB at 2710.508 kb on - strand, within dapB at 2710.539 kb on + strand, within dapB at 2710.539 kb on + strand, within dapB at 2710.540 kb on - strand, within dapB at 2710.540 kb on - strand, within dapB at 2710.540 kb on - strand, within dapB at 2710.603 kb on + strand, within dapB at 2710.603 kb on + strand, within dapB at 2710.603 kb on + strand, within dapB at 2710.603 kb on + strand, within dapB at 2710.603 kb on + strand, within dapB at 2710.603 kb on + strand, within dapB at 2710.603 kb on + strand, within dapB at 2710.604 kb on - strand, within dapB at 2710.604 kb on - strand, within dapB at 2710.604 kb on - strand, within dapB at 2710.604 kb on - strand, within dapB at 2710.604 kb on - strand, within dapB at 2710.647 kb on - strand, within dapB at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.889 kb on + strand at 2710.890 kb on - strand at 2710.890 kb on - strand at 2710.890 kb on - strand at 2710.890 kb on - strand at 2710.890 kb on - strand at 2710.890 kb on - strand at 2710.890 kb on - strand at 2710.890 kb on - strand at 2710.890 kb on - strand at 2710.890 kb on - strand at 2710.890 kb on - strand at 2710.890 kb on - strand at 2710.913 kb on + strand at 2710.913 kb on + strand at 2711.531 kb on + strand, within lpdA at 2711.709 kb on - strand, within lpdA at 2711.870 kb on + strand, within lpdA at 2712.233 kb on + strand, within lpdA at 2712.474 kb on + strand, within lpdA at 2712.475 kb on - strand, within lpdA at 2712.761 kb on + strand at 2712.884 kb on + strand, within aceF at 2712.884 kb on + strand, within aceF at 2712.885 kb on - strand, within aceF at 2712.885 kb on - strand, within aceF at 2712.885 kb on - strand, within aceF at 2713.083 kb on + strand, within aceF at 2713.085 kb on + strand, within aceF at 2713.467 kb on - strand, within aceF
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 1024 uM remove 2,709,879 - -0.2 2,710,024 + +0.5 2,710,024 + -1.1 2,710,024 + +1.6 2,710,024 + +0.8 2,710,024 + +0.1 2,710,024 + +0.1 2,710,024 + +0.3 2,710,025 - +0.2 2,710,025 - -0.5 2,710,025 - -0.9 2,710,025 - -2.2 2,710,025 - -0.1 2,710,108 + dapB OKGIIK_12585 0.11 -1.7 2,710,108 + dapB OKGIIK_12585 0.11 +0.1 2,710,108 + dapB OKGIIK_12585 0.11 -0.5 2,710,108 + dapB OKGIIK_12585 0.11 +0.9 2,710,109 - dapB OKGIIK_12585 0.11 -1.0 2,710,188 + dapB OKGIIK_12585 0.21 -0.7 2,710,188 + dapB OKGIIK_12585 0.21 +0.5 2,710,188 + dapB OKGIIK_12585 0.21 -0.4 2,710,189 - dapB OKGIIK_12585 0.21 +0.4 2,710,189 - dapB OKGIIK_12585 0.21 -0.4 2,710,329 + dapB OKGIIK_12585 0.38 -0.6 2,710,330 - dapB OKGIIK_12585 0.38 -2.2 2,710,330 - dapB OKGIIK_12585 0.38 +0.5 2,710,330 - dapB OKGIIK_12585 0.38 -0.7 2,710,330 - dapB OKGIIK_12585 0.38 -1.9 2,710,330 - dapB OKGIIK_12585 0.38 -0.4 2,710,330 - dapB OKGIIK_12585 0.38 +0.9 2,710,363 + dapB OKGIIK_12585 0.42 -1.2 2,710,369 + dapB OKGIIK_12585 0.43 -0.1 2,710,369 + dapB OKGIIK_12585 0.43 -0.3 2,710,369 + dapB OKGIIK_12585 0.43 -0.1 2,710,370 - dapB OKGIIK_12585 0.43 +1.4 2,710,370 - dapB OKGIIK_12585 0.43 +0.7 2,710,370 - dapB OKGIIK_12585 0.43 -0.1 2,710,370 - dapB OKGIIK_12585 0.43 -0.1 2,710,370 - dapB OKGIIK_12585 0.43 -2.6 2,710,370 - dapB OKGIIK_12585 0.43 -0.5 2,710,370 - dapB OKGIIK_12585 0.43 +0.3 2,710,370 - dapB OKGIIK_12585 0.43 +0.3 2,710,507 + dapB OKGIIK_12585 0.60 -0.4 2,710,507 + dapB OKGIIK_12585 0.60 -0.8 2,710,508 - dapB OKGIIK_12585 0.61 +0.1 2,710,508 - dapB OKGIIK_12585 0.61 -1.5 2,710,508 - dapB OKGIIK_12585 0.61 -0.1 2,710,508 - dapB OKGIIK_12585 0.61 -1.2 2,710,539 + dapB OKGIIK_12585 0.64 -0.4 2,710,539 + dapB OKGIIK_12585 0.64 +1.0 2,710,540 - dapB OKGIIK_12585 0.65 +0.2 2,710,540 - dapB OKGIIK_12585 0.65 -0.4 2,710,540 - dapB OKGIIK_12585 0.65 -0.8 2,710,603 + dapB OKGIIK_12585 0.72 +0.3 2,710,603 + dapB OKGIIK_12585 0.72 -0.6 2,710,603 + dapB OKGIIK_12585 0.72 -2.4 2,710,603 + dapB OKGIIK_12585 0.72 +0.8 2,710,603 + dapB OKGIIK_12585 0.72 -1.2 2,710,603 + dapB OKGIIK_12585 0.72 -1.0 2,710,603 + dapB OKGIIK_12585 0.72 +0.5 2,710,604 - dapB OKGIIK_12585 0.73 +2.3 2,710,604 - dapB OKGIIK_12585 0.73 -0.5 2,710,604 - dapB OKGIIK_12585 0.73 -0.0 2,710,604 - dapB OKGIIK_12585 0.73 +0.0 2,710,604 - dapB OKGIIK_12585 0.73 -2.1 2,710,647 - dapB OKGIIK_12585 0.78 -1.2 2,710,889 + -0.1 2,710,889 + +0.3 2,710,889 + +0.0 2,710,889 + -1.4 2,710,889 + -0.8 2,710,889 + -0.1 2,710,889 + +1.8 2,710,889 + -0.5 2,710,889 + -0.1 2,710,889 + -0.9 2,710,889 + +2.4 2,710,889 + -0.1 2,710,889 + +1.2 2,710,889 + -0.2 2,710,889 + -1.1 2,710,889 + +0.9 2,710,889 + +0.9 2,710,889 + +0.1 2,710,889 + -0.0 2,710,890 - -2.9 2,710,890 - -0.1 2,710,890 - -0.2 2,710,890 - +1.1 2,710,890 - +0.1 2,710,890 - +0.9 2,710,890 - -1.4 2,710,890 - +0.4 2,710,890 - -1.4 2,710,890 - -0.4 2,710,890 - -1.4 2,710,890 - -0.5 2,710,913 + +0.7 2,710,913 + -0.4 2,711,531 + lpdA OKGIIK_12590 0.37 -0.2 2,711,709 - lpdA OKGIIK_12590 0.47 -0.4 2,711,870 + lpdA OKGIIK_12590 0.56 -0.1 2,712,233 + lpdA OKGIIK_12590 0.76 -0.6 2,712,474 + lpdA OKGIIK_12590 0.90 +0.3 2,712,475 - lpdA OKGIIK_12590 0.90 -0.7 2,712,761 + -1.7 2,712,884 + aceF OKGIIK_12595 0.15 -1.9 2,712,884 + aceF OKGIIK_12595 0.15 -0.4 2,712,885 - aceF OKGIIK_12595 0.15 -0.2 2,712,885 - aceF OKGIIK_12595 0.15 -0.9 2,712,885 - aceF OKGIIK_12595 0.15 -0.7 2,713,083 + aceF OKGIIK_12595 0.29 -0.7 2,713,085 + aceF OKGIIK_12595 0.29 -0.6 2,713,467 - aceF OKGIIK_12595 0.57 -0.9
Or see this region's nucleotide sequence