Experiment: R2A_PIPES with Cobalt chloride 1024 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proA and proB overlap by 4 nucleotides proB and OKGIIK_09425 overlap by 4 nucleotides OKGIIK_09425 and argH are separated by 12 nucleotides
OKGIIK_09415: proA - glutamate-5-semialdehyde dehydrogenase, at 2,042,854 to 2,044,116
proA
OKGIIK_09420: proB - glutamate 5-kinase, at 2,044,113 to 2,045,273
proB
OKGIIK_09425: OKGIIK_09425 - Cupin, at 2,045,270 to 2,045,632
_09425
OKGIIK_09430: argH - argininosuccinate lyase, at 2,045,645 to 2,046,940
argH
Position (kb)
2044
2045
2046 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2043.116 kb on + strand, within proA at 2043.197 kb on + strand, within proA at 2043.197 kb on + strand, within proA at 2043.198 kb on - strand, within proA at 2043.428 kb on + strand, within proA at 2043.428 kb on + strand, within proA at 2043.428 kb on + strand, within proA at 2043.429 kb on - strand, within proA at 2043.429 kb on - strand, within proA at 2043.429 kb on - strand, within proA at 2043.429 kb on - strand, within proA at 2043.429 kb on - strand, within proA at 2043.429 kb on - strand, within proA at 2043.429 kb on - strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.449 kb on + strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.450 kb on - strand, within proA at 2043.755 kb on + strand, within proA at 2043.755 kb on + strand, within proA at 2043.755 kb on + strand, within proA at 2043.755 kb on + strand, within proA at 2043.755 kb on + strand, within proA at 2043.755 kb on + strand, within proA at 2043.756 kb on - strand, within proA at 2043.756 kb on - strand, within proA at 2043.756 kb on - strand, within proA at 2043.756 kb on - strand, within proA at 2043.756 kb on - strand, within proA at 2044.057 kb on + strand at 2044.057 kb on + strand at 2044.057 kb on + strand at 2044.057 kb on + strand at 2044.057 kb on + strand at 2044.057 kb on + strand at 2044.058 kb on - strand at 2044.198 kb on + strand at 2044.264 kb on + strand, within proB at 2044.265 kb on - strand, within proB at 2044.265 kb on - strand, within proB at 2044.265 kb on - strand, within proB at 2044.456 kb on + strand, within proB at 2044.456 kb on + strand, within proB at 2044.456 kb on + strand, within proB at 2044.732 kb on + strand, within proB at 2044.733 kb on - strand, within proB at 2044.733 kb on - strand, within proB at 2044.915 kb on + strand, within proB at 2044.916 kb on - strand, within proB at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.469 kb on + strand, within OKGIIK_09425 at 2045.470 kb on - strand, within OKGIIK_09425 at 2045.598 kb on + strand at 2045.598 kb on + strand at 2045.598 kb on + strand at 2045.598 kb on + strand at 2045.598 kb on + strand at 2045.599 kb on - strand at 2045.599 kb on - strand at 2045.665 kb on + strand at 2045.665 kb on + strand at 2045.844 kb on + strand, within argH at 2045.844 kb on + strand, within argH at 2045.844 kb on + strand, within argH at 2045.844 kb on + strand, within argH at 2045.845 kb on - strand, within argH at 2045.845 kb on - strand, within argH at 2045.845 kb on - strand, within argH at 2045.845 kb on - strand, within argH at 2045.845 kb on - strand, within argH at 2045.845 kb on - strand, within argH at 2045.845 kb on - strand, within argH at 2046.048 kb on + strand, within argH at 2046.049 kb on - strand, within argH at 2046.049 kb on - strand, within argH at 2046.049 kb on - strand, within argH at 2046.049 kb on - strand, within argH at 2046.049 kb on - strand, within argH at 2046.117 kb on + strand, within argH at 2046.118 kb on - strand, within argH at 2046.118 kb on - strand, within argH at 2046.259 kb on - strand, within argH at 2046.259 kb on - strand, within argH
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 1024 uM remove 2,043,116 + proA OKGIIK_09415 0.21 +0.4 2,043,197 + proA OKGIIK_09415 0.27 -1.1 2,043,197 + proA OKGIIK_09415 0.27 -0.7 2,043,198 - proA OKGIIK_09415 0.27 -0.7 2,043,428 + proA OKGIIK_09415 0.45 -1.7 2,043,428 + proA OKGIIK_09415 0.45 +2.0 2,043,428 + proA OKGIIK_09415 0.45 +0.7 2,043,429 - proA OKGIIK_09415 0.46 +2.3 2,043,429 - proA OKGIIK_09415 0.46 -0.7 2,043,429 - proA OKGIIK_09415 0.46 +0.6 2,043,429 - proA OKGIIK_09415 0.46 -0.4 2,043,429 - proA OKGIIK_09415 0.46 +1.0 2,043,429 - proA OKGIIK_09415 0.46 -0.4 2,043,429 - proA OKGIIK_09415 0.46 +0.1 2,043,449 + proA OKGIIK_09415 0.47 -0.5 2,043,449 + proA OKGIIK_09415 0.47 -0.0 2,043,449 + proA OKGIIK_09415 0.47 -0.0 2,043,449 + proA OKGIIK_09415 0.47 +0.2 2,043,449 + proA OKGIIK_09415 0.47 -1.0 2,043,449 + proA OKGIIK_09415 0.47 -1.0 2,043,449 + proA OKGIIK_09415 0.47 -1.4 2,043,449 + proA OKGIIK_09415 0.47 -1.0 2,043,449 + proA OKGIIK_09415 0.47 +0.7 2,043,449 + proA OKGIIK_09415 0.47 +0.2 2,043,449 + proA OKGIIK_09415 0.47 -1.2 2,043,449 + proA OKGIIK_09415 0.47 -0.0 2,043,449 + proA OKGIIK_09415 0.47 -1.5 2,043,449 + proA OKGIIK_09415 0.47 -0.4 2,043,449 + proA OKGIIK_09415 0.47 -0.0 2,043,449 + proA OKGIIK_09415 0.47 +0.1 2,043,449 + proA OKGIIK_09415 0.47 -0.6 2,043,450 - proA OKGIIK_09415 0.47 -0.2 2,043,450 - proA OKGIIK_09415 0.47 -1.2 2,043,450 - proA OKGIIK_09415 0.47 -2.0 2,043,450 - proA OKGIIK_09415 0.47 -1.2 2,043,450 - proA OKGIIK_09415 0.47 -0.1 2,043,450 - proA OKGIIK_09415 0.47 -1.1 2,043,450 - proA OKGIIK_09415 0.47 -0.0 2,043,450 - proA OKGIIK_09415 0.47 -0.2 2,043,450 - proA OKGIIK_09415 0.47 +1.0 2,043,755 + proA OKGIIK_09415 0.71 +0.1 2,043,755 + proA OKGIIK_09415 0.71 -0.4 2,043,755 + proA OKGIIK_09415 0.71 -0.8 2,043,755 + proA OKGIIK_09415 0.71 -0.8 2,043,755 + proA OKGIIK_09415 0.71 -1.0 2,043,755 + proA OKGIIK_09415 0.71 -1.0 2,043,756 - proA OKGIIK_09415 0.71 -0.0 2,043,756 - proA OKGIIK_09415 0.71 +2.2 2,043,756 - proA OKGIIK_09415 0.71 -0.6 2,043,756 - proA OKGIIK_09415 0.71 +1.0 2,043,756 - proA OKGIIK_09415 0.71 -1.0 2,044,057 + +1.0 2,044,057 + +0.1 2,044,057 + +1.6 2,044,057 + -0.4 2,044,057 + -0.4 2,044,057 + +0.6 2,044,058 - -1.2 2,044,198 + -2.4 2,044,264 + proB OKGIIK_09420 0.13 -0.4 2,044,265 - proB OKGIIK_09420 0.13 -0.6 2,044,265 - proB OKGIIK_09420 0.13 +0.7 2,044,265 - proB OKGIIK_09420 0.13 +0.2 2,044,456 + proB OKGIIK_09420 0.30 -0.4 2,044,456 + proB OKGIIK_09420 0.30 -0.0 2,044,456 + proB OKGIIK_09420 0.30 +0.2 2,044,732 + proB OKGIIK_09420 0.53 -0.4 2,044,733 - proB OKGIIK_09420 0.53 -0.2 2,044,733 - proB OKGIIK_09420 0.53 +1.2 2,044,915 + proB OKGIIK_09420 0.69 +0.6 2,044,916 - proB OKGIIK_09420 0.69 -0.6 2,045,469 + OKGIIK_09425 0.55 +1.4 2,045,469 + OKGIIK_09425 0.55 +0.7 2,045,469 + OKGIIK_09425 0.55 +1.7 2,045,469 + OKGIIK_09425 0.55 -0.8 2,045,469 + OKGIIK_09425 0.55 +0.8 2,045,469 + OKGIIK_09425 0.55 -0.0 2,045,469 + OKGIIK_09425 0.55 +0.8 2,045,469 + OKGIIK_09425 0.55 +1.3 2,045,469 + OKGIIK_09425 0.55 -0.6 2,045,470 - OKGIIK_09425 0.55 -1.1 2,045,598 + -0.9 2,045,598 + -0.8 2,045,598 + +1.2 2,045,598 + -0.4 2,045,598 + +0.3 2,045,599 - -1.2 2,045,599 - +0.5 2,045,665 + -1.4 2,045,665 + -2.1 2,045,844 + argH OKGIIK_09430 0.15 -0.4 2,045,844 + argH OKGIIK_09430 0.15 -2.4 2,045,844 + argH OKGIIK_09430 0.15 -1.5 2,045,844 + argH OKGIIK_09430 0.15 +0.5 2,045,845 - argH OKGIIK_09430 0.15 -1.2 2,045,845 - argH OKGIIK_09430 0.15 +1.6 2,045,845 - argH OKGIIK_09430 0.15 -0.7 2,045,845 - argH OKGIIK_09430 0.15 -0.4 2,045,845 - argH OKGIIK_09430 0.15 +0.4 2,045,845 - argH OKGIIK_09430 0.15 -3.2 2,045,845 - argH OKGIIK_09430 0.15 -1.5 2,046,048 + argH OKGIIK_09430 0.31 +1.6 2,046,049 - argH OKGIIK_09430 0.31 +1.6 2,046,049 - argH OKGIIK_09430 0.31 -0.6 2,046,049 - argH OKGIIK_09430 0.31 -1.0 2,046,049 - argH OKGIIK_09430 0.31 -0.8 2,046,049 - argH OKGIIK_09430 0.31 -0.6 2,046,117 + argH OKGIIK_09430 0.36 +0.1 2,046,118 - argH OKGIIK_09430 0.36 -1.7 2,046,118 - argH OKGIIK_09430 0.36 -1.6 2,046,259 - argH OKGIIK_09430 0.47 +0.6 2,046,259 - argH OKGIIK_09430 0.47 -2.1
Or see this region's nucleotide sequence