Experiment: R2A_PIPES with Cobalt chloride 1024 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppk1 and phoR are separated by 54 nucleotides phoR and phoB are separated by 40 nucleotides phoB and OKGIIK_03795 are separated by 90 nucleotides
OKGIIK_03780: ppk1 - polyphosphate kinase 1, at 833,553 to 835,646
ppk1
OKGIIK_03785: phoR - phosphate regulon sensor histidine kinase PhoR, at 835,701 to 837,008
phoR
OKGIIK_03790: phoB - phosphate regulon transcriptional regulator PhoB, at 837,049 to 837,738
phoB
OKGIIK_03795: OKGIIK_03795 - Putative beta-barrel assembly-enhancing protease, at 837,829 to 839,532
_03795
Position (kb) 835
836
837
838 Strain fitness (log2 ratio) -2
-1
0
1
2 at 834.740 kb on - strand, within ppk1 at 834.740 kb on - strand, within ppk1 at 834.740 kb on - strand, within ppk1 at 835.222 kb on + strand, within ppk1 at 835.222 kb on + strand, within ppk1 at 835.222 kb on + strand, within ppk1 at 835.222 kb on + strand, within ppk1 at 835.223 kb on - strand, within ppk1 at 835.327 kb on + strand, within ppk1 at 835.327 kb on + strand, within ppk1 at 835.327 kb on + strand, within ppk1 at 835.327 kb on + strand, within ppk1 at 835.328 kb on - strand, within ppk1 at 835.328 kb on - strand, within ppk1 at 835.328 kb on - strand, within ppk1 at 835.328 kb on - strand, within ppk1 at 835.421 kb on - strand, within ppk1 at 835.421 kb on - strand, within ppk1 at 835.421 kb on - strand, within ppk1 at 835.496 kb on - strand at 835.496 kb on - strand at 835.523 kb on + strand at 835.524 kb on - strand at 835.588 kb on + strand at 835.588 kb on + strand at 835.588 kb on + strand at 835.588 kb on + strand at 835.588 kb on + strand at 835.588 kb on + strand at 835.588 kb on + strand at 835.589 kb on - strand at 835.589 kb on - strand at 835.589 kb on - strand at 835.589 kb on - strand at 835.589 kb on - strand at 835.589 kb on - strand at 835.589 kb on - strand at 835.589 kb on - strand at 835.888 kb on + strand, within phoR at 835.889 kb on - strand, within phoR at 835.954 kb on + strand, within phoR at 835.954 kb on + strand, within phoR at 835.955 kb on - strand, within phoR at 836.014 kb on + strand, within phoR at 836.015 kb on - strand, within phoR at 836.326 kb on + strand, within phoR at 837.173 kb on + strand, within phoB at 837.173 kb on + strand, within phoB at 837.173 kb on + strand, within phoB at 837.173 kb on + strand, within phoB at 837.173 kb on + strand, within phoB at 837.173 kb on + strand, within phoB at 837.173 kb on + strand, within phoB at 837.173 kb on + strand, within phoB at 837.174 kb on - strand, within phoB at 837.174 kb on - strand, within phoB at 837.174 kb on - strand, within phoB at 837.174 kb on - strand, within phoB at 837.174 kb on - strand, within phoB at 837.174 kb on - strand, within phoB at 837.218 kb on + strand, within phoB at 837.219 kb on - strand, within phoB at 837.219 kb on - strand, within phoB at 837.248 kb on + strand, within phoB at 837.248 kb on + strand, within phoB at 837.248 kb on + strand, within phoB at 837.248 kb on + strand, within phoB at 837.248 kb on + strand, within phoB at 837.249 kb on - strand, within phoB at 837.249 kb on - strand, within phoB at 837.249 kb on - strand, within phoB at 837.249 kb on - strand, within phoB at 837.249 kb on - strand, within phoB at 837.249 kb on - strand, within phoB at 837.434 kb on + strand, within phoB at 837.434 kb on + strand, within phoB at 837.434 kb on + strand, within phoB at 837.434 kb on + strand, within phoB at 837.434 kb on + strand, within phoB at 837.434 kb on + strand, within phoB at 837.435 kb on - strand, within phoB at 837.435 kb on - strand, within phoB at 837.435 kb on - strand, within phoB at 837.435 kb on - strand, within phoB at 837.752 kb on + strand at 837.752 kb on + strand at 837.752 kb on + strand at 837.752 kb on + strand at 837.752 kb on + strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.753 kb on - strand at 837.770 kb on + strand at 837.770 kb on + strand at 837.770 kb on + strand at 837.770 kb on + strand at 837.770 kb on + strand at 837.770 kb on + strand at 837.770 kb on + strand at 837.770 kb on + strand at 837.770 kb on + strand at 837.770 kb on + strand at 837.770 kb on + strand at 837.771 kb on - strand at 837.771 kb on - strand at 837.771 kb on - strand at 837.771 kb on - strand at 837.771 kb on - strand at 837.789 kb on + strand at 837.789 kb on + strand at 837.789 kb on + strand at 837.789 kb on + strand at 837.789 kb on + strand at 837.789 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 1024 uM remove 834,740 - ppk1 OKGIIK_03780 0.57 -2.0 834,740 - ppk1 OKGIIK_03780 0.57 -1.9 834,740 - ppk1 OKGIIK_03780 0.57 +0.7 835,222 + ppk1 OKGIIK_03780 0.80 +0.3 835,222 + ppk1 OKGIIK_03780 0.80 -0.7 835,222 + ppk1 OKGIIK_03780 0.80 +1.2 835,222 + ppk1 OKGIIK_03780 0.80 -0.1 835,223 - ppk1 OKGIIK_03780 0.80 -0.2 835,327 + ppk1 OKGIIK_03780 0.85 -0.3 835,327 + ppk1 OKGIIK_03780 0.85 +1.3 835,327 + ppk1 OKGIIK_03780 0.85 -1.5 835,327 + ppk1 OKGIIK_03780 0.85 +0.9 835,328 - ppk1 OKGIIK_03780 0.85 +1.3 835,328 - ppk1 OKGIIK_03780 0.85 -0.1 835,328 - ppk1 OKGIIK_03780 0.85 -0.4 835,328 - ppk1 OKGIIK_03780 0.85 -0.4 835,421 - ppk1 OKGIIK_03780 0.89 -1.2 835,421 - ppk1 OKGIIK_03780 0.89 +0.6 835,421 - ppk1 OKGIIK_03780 0.89 +0.7 835,496 - -0.4 835,496 - +1.3 835,523 + -0.7 835,524 - +1.3 835,588 + -0.8 835,588 + +0.1 835,588 + -0.2 835,588 + -0.4 835,588 + +0.4 835,588 + +0.4 835,588 + -0.2 835,589 - -0.7 835,589 - -0.9 835,589 - +0.9 835,589 - -0.3 835,589 - -1.2 835,589 - -1.0 835,589 - -0.1 835,589 - -0.1 835,888 + phoR OKGIIK_03785 0.14 +0.1 835,889 - phoR OKGIIK_03785 0.14 -0.1 835,954 + phoR OKGIIK_03785 0.19 +1.1 835,954 + phoR OKGIIK_03785 0.19 +0.5 835,955 - phoR OKGIIK_03785 0.19 +0.3 836,014 + phoR OKGIIK_03785 0.24 +0.7 836,015 - phoR OKGIIK_03785 0.24 -0.0 836,326 + phoR OKGIIK_03785 0.48 +0.0 837,173 + phoB OKGIIK_03790 0.18 +0.2 837,173 + phoB OKGIIK_03790 0.18 -0.4 837,173 + phoB OKGIIK_03790 0.18 +1.2 837,173 + phoB OKGIIK_03790 0.18 +0.7 837,173 + phoB OKGIIK_03790 0.18 +2.2 837,173 + phoB OKGIIK_03790 0.18 -0.3 837,173 + phoB OKGIIK_03790 0.18 +0.3 837,173 + phoB OKGIIK_03790 0.18 +1.1 837,174 - phoB OKGIIK_03790 0.18 +0.3 837,174 - phoB OKGIIK_03790 0.18 +0.5 837,174 - phoB OKGIIK_03790 0.18 +0.3 837,174 - phoB OKGIIK_03790 0.18 -2.0 837,174 - phoB OKGIIK_03790 0.18 -0.1 837,174 - phoB OKGIIK_03790 0.18 +0.9 837,218 + phoB OKGIIK_03790 0.24 -1.1 837,219 - phoB OKGIIK_03790 0.25 -1.8 837,219 - phoB OKGIIK_03790 0.25 +1.4 837,248 + phoB OKGIIK_03790 0.29 -1.1 837,248 + phoB OKGIIK_03790 0.29 -0.1 837,248 + phoB OKGIIK_03790 0.29 -0.2 837,248 + phoB OKGIIK_03790 0.29 +1.5 837,248 + phoB OKGIIK_03790 0.29 +1.5 837,249 - phoB OKGIIK_03790 0.29 +1.5 837,249 - phoB OKGIIK_03790 0.29 +1.0 837,249 - phoB OKGIIK_03790 0.29 -0.5 837,249 - phoB OKGIIK_03790 0.29 -0.5 837,249 - phoB OKGIIK_03790 0.29 -0.5 837,249 - phoB OKGIIK_03790 0.29 -0.2 837,434 + phoB OKGIIK_03790 0.56 +0.2 837,434 + phoB OKGIIK_03790 0.56 +1.9 837,434 + phoB OKGIIK_03790 0.56 -1.1 837,434 + phoB OKGIIK_03790 0.56 -0.8 837,434 + phoB OKGIIK_03790 0.56 -0.1 837,434 + phoB OKGIIK_03790 0.56 -0.5 837,435 - phoB OKGIIK_03790 0.56 +0.0 837,435 - phoB OKGIIK_03790 0.56 -0.1 837,435 - phoB OKGIIK_03790 0.56 +0.3 837,435 - phoB OKGIIK_03790 0.56 -0.8 837,752 + -1.7 837,752 + +0.1 837,752 + +0.6 837,752 + -0.5 837,752 + -0.4 837,753 - -0.7 837,753 - +0.9 837,753 - -0.7 837,753 - +0.6 837,753 - -0.1 837,753 - +0.7 837,753 - +1.2 837,753 - -0.9 837,753 - -0.9 837,753 - -0.8 837,753 - +0.8 837,753 - -1.8 837,753 - -0.7 837,753 - -0.4 837,753 - +1.4 837,753 - +0.4 837,753 - +1.6 837,753 - -0.4 837,753 - -2.6 837,753 - +1.9 837,753 - -0.4 837,753 - +0.5 837,753 - -0.5 837,753 - -0.3 837,753 - +0.2 837,753 - -0.4 837,753 - +0.4 837,770 + -0.9 837,770 + +0.1 837,770 + +1.1 837,770 + +0.5 837,770 + -0.2 837,770 + -0.1 837,770 + +1.1 837,770 + +1.4 837,770 + -1.5 837,770 + -0.9 837,770 + -0.5 837,771 - +1.9 837,771 - +0.2 837,771 - -0.2 837,771 - -1.3 837,771 - -0.7 837,789 + +0.9 837,789 + +0.3 837,789 + +0.9 837,789 + -0.4 837,789 + +0.3 837,789 + -0.3
Or see this region's nucleotide sequence