Experiment: R2A_PIPES with Cobalt chloride 1024 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgJ and OKGIIK_02695 overlap by 4 nucleotides OKGIIK_02695 and flgK overlap by 8 nucleotides flgK and flgL are separated by 3 nucleotides
OKGIIK_02690: flgJ - flagellar assembly peptidoglycan hydrolase FlgJ, at 623,747 to 624,814
flgJ
OKGIIK_02695: OKGIIK_02695 - hypothetical protein, at 624,811 to 625,461
_02695
OKGIIK_02700: flgK - flagellar hook-associated protein FlgK, at 625,454 to 627,328
flgK
OKGIIK_02705: flgL - flagellar hook-associated protein FlgL, at 627,332 to 628,534
flgL
Position (kb) 625
626
627
628 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 624.539 kb on + strand, within flgJ at 624.539 kb on + strand, within flgJ at 624.539 kb on + strand, within flgJ at 624.540 kb on - strand, within flgJ at 624.584 kb on + strand, within flgJ at 624.584 kb on + strand, within flgJ at 624.585 kb on - strand, within flgJ at 624.614 kb on + strand, within flgJ at 624.615 kb on - strand, within flgJ at 624.644 kb on + strand, within flgJ at 624.710 kb on + strand at 624.728 kb on + strand at 624.728 kb on + strand at 624.728 kb on + strand at 624.728 kb on + strand at 624.728 kb on + strand at 624.728 kb on + strand at 624.728 kb on + strand at 624.729 kb on - strand at 624.729 kb on - strand at 624.729 kb on - strand at 624.729 kb on - strand at 625.467 kb on + strand at 625.467 kb on + strand at 625.467 kb on + strand at 625.479 kb on + strand at 625.480 kb on - strand at 625.543 kb on + strand at 625.543 kb on + strand at 625.544 kb on - strand at 625.554 kb on - strand at 625.574 kb on + strand at 625.575 kb on - strand at 625.575 kb on - strand at 625.575 kb on - strand at 625.608 kb on - strand at 625.608 kb on - strand at 625.610 kb on + strand at 625.610 kb on + strand at 625.610 kb on + strand at 625.610 kb on + strand at 625.610 kb on + strand at 625.610 kb on + strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.652 kb on + strand, within flgK at 625.653 kb on - strand, within flgK at 625.653 kb on - strand, within flgK at 625.653 kb on - strand, within flgK at 625.653 kb on - strand, within flgK at 625.845 kb on + strand, within flgK at 625.845 kb on + strand, within flgK at 625.845 kb on + strand, within flgK at 625.845 kb on + strand, within flgK at 625.846 kb on - strand, within flgK at 625.846 kb on - strand, within flgK at 625.846 kb on - strand, within flgK at 625.846 kb on - strand, within flgK at 625.846 kb on - strand, within flgK at 625.846 kb on - strand, within flgK at 625.923 kb on + strand, within flgK at 625.923 kb on + strand, within flgK at 625.923 kb on + strand, within flgK at 625.923 kb on + strand, within flgK at 625.923 kb on + strand, within flgK at 625.923 kb on + strand, within flgK at 625.923 kb on + strand, within flgK at 625.923 kb on + strand, within flgK at 625.923 kb on + strand, within flgK at 625.924 kb on - strand, within flgK at 625.924 kb on - strand, within flgK at 625.924 kb on - strand, within flgK at 625.924 kb on - strand, within flgK at 625.924 kb on - strand, within flgK at 625.924 kb on - strand, within flgK at 625.924 kb on - strand, within flgK at 625.924 kb on - strand, within flgK at 625.924 kb on - strand, within flgK at 625.924 kb on - strand, within flgK at 625.924 kb on - strand, within flgK at 626.101 kb on - strand, within flgK at 626.198 kb on + strand, within flgK at 626.198 kb on + strand, within flgK at 626.198 kb on + strand, within flgK at 626.198 kb on + strand, within flgK at 626.199 kb on - strand, within flgK at 626.199 kb on - strand, within flgK at 626.199 kb on - strand, within flgK at 626.199 kb on - strand, within flgK at 626.519 kb on + strand, within flgK at 626.519 kb on + strand, within flgK at 626.520 kb on - strand, within flgK at 626.520 kb on - strand, within flgK at 626.520 kb on - strand, within flgK at 626.520 kb on - strand, within flgK at 626.520 kb on - strand, within flgK at 626.520 kb on - strand, within flgK at 626.520 kb on - strand, within flgK at 626.520 kb on - strand, within flgK at 626.531 kb on + strand, within flgK at 626.531 kb on + strand, within flgK at 626.531 kb on + strand, within flgK at 626.531 kb on + strand, within flgK at 626.531 kb on + strand, within flgK at 626.532 kb on - strand, within flgK at 626.532 kb on - strand, within flgK at 626.532 kb on - strand, within flgK at 626.779 kb on + strand, within flgK at 626.873 kb on + strand, within flgK at 626.873 kb on + strand, within flgK at 626.873 kb on + strand, within flgK at 626.873 kb on + strand, within flgK at 626.873 kb on + strand, within flgK at 626.873 kb on + strand, within flgK at 626.873 kb on + strand, within flgK at 626.873 kb on + strand, within flgK at 626.873 kb on + strand, within flgK at 626.873 kb on + strand, within flgK at 626.873 kb on + strand, within flgK at 626.873 kb on + strand, within flgK at 626.874 kb on - strand, within flgK at 626.874 kb on - strand, within flgK at 626.874 kb on - strand, within flgK at 626.874 kb on - strand, within flgK at 626.874 kb on - strand, within flgK at 626.874 kb on - strand, within flgK at 626.874 kb on - strand, within flgK at 626.874 kb on - strand, within flgK at 626.985 kb on + strand, within flgK at 626.986 kb on - strand, within flgK at 626.986 kb on - strand, within flgK at 626.986 kb on - strand, within flgK at 626.986 kb on - strand, within flgK at 626.986 kb on - strand, within flgK at 626.986 kb on - strand, within flgK at 626.986 kb on - strand, within flgK at 626.986 kb on - strand, within flgK at 626.986 kb on - strand, within flgK at 626.986 kb on - strand, within flgK at 626.986 kb on - strand, within flgK at 627.167 kb on + strand at 627.167 kb on + strand at 627.167 kb on + strand at 627.167 kb on + strand at 627.168 kb on - strand at 627.168 kb on - strand at 627.168 kb on - strand at 627.168 kb on - strand at 627.168 kb on - strand at 627.168 kb on - strand at 627.254 kb on + strand at 627.254 kb on + strand at 627.254 kb on + strand at 627.254 kb on + strand at 627.254 kb on + strand at 627.255 kb on - strand at 627.255 kb on - strand at 627.326 kb on + strand at 627.326 kb on + strand at 627.412 kb on + strand at 627.412 kb on + strand at 627.412 kb on + strand at 627.412 kb on + strand at 627.412 kb on + strand at 627.412 kb on + strand at 627.412 kb on + strand at 627.413 kb on - strand at 627.413 kb on - strand at 627.413 kb on - strand at 627.413 kb on - strand at 627.515 kb on + strand, within flgL at 627.515 kb on + strand, within flgL at 627.516 kb on - strand, within flgL at 627.516 kb on - strand, within flgL at 627.731 kb on + strand, within flgL at 627.731 kb on + strand, within flgL at 627.731 kb on + strand, within flgL at 627.731 kb on + strand, within flgL at 627.731 kb on + strand, within flgL at 627.732 kb on - strand, within flgL at 627.732 kb on - strand, within flgL at 627.732 kb on - strand, within flgL at 627.732 kb on - strand, within flgL at 627.732 kb on - strand, within flgL at 627.732 kb on - strand, within flgL at 627.791 kb on + strand, within flgL at 627.791 kb on + strand, within flgL at 627.791 kb on + strand, within flgL at 627.791 kb on + strand, within flgL at 627.792 kb on - strand, within flgL at 627.792 kb on - strand, within flgL at 627.792 kb on - strand, within flgL at 627.792 kb on - strand, within flgL at 627.792 kb on - strand, within flgL at 628.005 kb on - strand, within flgL at 628.005 kb on - strand, within flgL at 628.005 kb on - strand, within flgL at 628.005 kb on - strand, within flgL at 628.034 kb on + strand, within flgL at 628.034 kb on + strand, within flgL at 628.035 kb on - strand, within flgL at 628.035 kb on - strand, within flgL at 628.035 kb on - strand, within flgL at 628.035 kb on - strand, within flgL at 628.035 kb on - strand, within flgL at 628.035 kb on - strand, within flgL at 628.079 kb on + strand, within flgL at 628.079 kb on + strand, within flgL at 628.079 kb on + strand, within flgL at 628.080 kb on - strand, within flgL at 628.080 kb on - strand, within flgL at 628.125 kb on + strand, within flgL at 628.125 kb on + strand, within flgL at 628.126 kb on - strand, within flgL at 628.126 kb on - strand, within flgL at 628.126 kb on - strand, within flgL
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 1024 uM remove 624,539 + flgJ OKGIIK_02690 0.74 +1.9 624,539 + flgJ OKGIIK_02690 0.74 +0.3 624,539 + flgJ OKGIIK_02690 0.74 -1.5 624,540 - flgJ OKGIIK_02690 0.74 +0.3 624,584 + flgJ OKGIIK_02690 0.78 -0.7 624,584 + flgJ OKGIIK_02690 0.78 -0.8 624,585 - flgJ OKGIIK_02690 0.78 -0.8 624,614 + flgJ OKGIIK_02690 0.81 -0.2 624,615 - flgJ OKGIIK_02690 0.81 -0.5 624,644 + flgJ OKGIIK_02690 0.84 -0.7 624,710 + -1.4 624,728 + -0.1 624,728 + +0.9 624,728 + +2.1 624,728 + +0.5 624,728 + +0.6 624,728 + -1.4 624,728 + +0.2 624,729 - -1.0 624,729 - -0.2 624,729 - -1.2 624,729 - +1.7 625,467 + -0.1 625,467 + +1.7 625,467 + -0.1 625,479 + +1.6 625,480 - +1.2 625,543 + +1.6 625,543 + -0.1 625,544 - +1.2 625,554 - -1.5 625,574 + -1.2 625,575 - +1.9 625,575 - -1.8 625,575 - +0.2 625,608 - -1.5 625,608 - -1.3 625,610 + +0.9 625,610 + -0.1 625,610 + -0.7 625,610 + +0.1 625,610 + +0.3 625,610 + +0.0 625,611 - -1.4 625,611 - -1.0 625,611 - +1.0 625,611 - -1.7 625,611 - +0.9 625,611 - -0.2 625,611 - -1.2 625,611 - -0.6 625,611 - -0.1 625,611 - +0.0 625,611 - -1.1 625,611 - +0.0 625,611 - -0.1 625,611 - +0.1 625,652 + flgK OKGIIK_02700 0.11 +0.1 625,653 - flgK OKGIIK_02700 0.11 +0.0 625,653 - flgK OKGIIK_02700 0.11 +0.5 625,653 - flgK OKGIIK_02700 0.11 +0.1 625,653 - flgK OKGIIK_02700 0.11 -0.9 625,845 + flgK OKGIIK_02700 0.21 -0.4 625,845 + flgK OKGIIK_02700 0.21 -0.6 625,845 + flgK OKGIIK_02700 0.21 +0.5 625,845 + flgK OKGIIK_02700 0.21 -0.1 625,846 - flgK OKGIIK_02700 0.21 +0.6 625,846 - flgK OKGIIK_02700 0.21 +0.5 625,846 - flgK OKGIIK_02700 0.21 -0.6 625,846 - flgK OKGIIK_02700 0.21 -0.2 625,846 - flgK OKGIIK_02700 0.21 -0.7 625,846 - flgK OKGIIK_02700 0.21 -0.3 625,923 + flgK OKGIIK_02700 0.25 -1.3 625,923 + flgK OKGIIK_02700 0.25 +0.3 625,923 + flgK OKGIIK_02700 0.25 -0.5 625,923 + flgK OKGIIK_02700 0.25 -0.4 625,923 + flgK OKGIIK_02700 0.25 +1.6 625,923 + flgK OKGIIK_02700 0.25 +0.3 625,923 + flgK OKGIIK_02700 0.25 +1.0 625,923 + flgK OKGIIK_02700 0.25 -1.1 625,923 + flgK OKGIIK_02700 0.25 -0.4 625,924 - flgK OKGIIK_02700 0.25 -2.8 625,924 - flgK OKGIIK_02700 0.25 -0.7 625,924 - flgK OKGIIK_02700 0.25 -1.3 625,924 - flgK OKGIIK_02700 0.25 +0.8 625,924 - flgK OKGIIK_02700 0.25 +0.0 625,924 - flgK OKGIIK_02700 0.25 -0.8 625,924 - flgK OKGIIK_02700 0.25 -1.5 625,924 - flgK OKGIIK_02700 0.25 -0.9 625,924 - flgK OKGIIK_02700 0.25 +0.6 625,924 - flgK OKGIIK_02700 0.25 -0.1 625,924 - flgK OKGIIK_02700 0.25 -1.0 626,101 - flgK OKGIIK_02700 0.35 +1.4 626,198 + flgK OKGIIK_02700 0.40 -0.2 626,198 + flgK OKGIIK_02700 0.40 +0.3 626,198 + flgK OKGIIK_02700 0.40 +0.5 626,198 + flgK OKGIIK_02700 0.40 +0.2 626,199 - flgK OKGIIK_02700 0.40 +0.1 626,199 - flgK OKGIIK_02700 0.40 +0.2 626,199 - flgK OKGIIK_02700 0.40 -0.5 626,199 - flgK OKGIIK_02700 0.40 +0.5 626,519 + flgK OKGIIK_02700 0.57 -0.9 626,519 + flgK OKGIIK_02700 0.57 +0.6 626,520 - flgK OKGIIK_02700 0.57 +0.1 626,520 - flgK OKGIIK_02700 0.57 -0.2 626,520 - flgK OKGIIK_02700 0.57 -1.3 626,520 - flgK OKGIIK_02700 0.57 -0.1 626,520 - flgK OKGIIK_02700 0.57 +1.1 626,520 - flgK OKGIIK_02700 0.57 -0.7 626,520 - flgK OKGIIK_02700 0.57 +0.5 626,520 - flgK OKGIIK_02700 0.57 -0.1 626,531 + flgK OKGIIK_02700 0.57 +0.6 626,531 + flgK OKGIIK_02700 0.57 -0.7 626,531 + flgK OKGIIK_02700 0.57 +0.3 626,531 + flgK OKGIIK_02700 0.57 +0.5 626,531 + flgK OKGIIK_02700 0.57 -0.3 626,532 - flgK OKGIIK_02700 0.57 -1.3 626,532 - flgK OKGIIK_02700 0.57 +0.2 626,532 - flgK OKGIIK_02700 0.57 +1.7 626,779 + flgK OKGIIK_02700 0.71 -0.6 626,873 + flgK OKGIIK_02700 0.76 -0.9 626,873 + flgK OKGIIK_02700 0.76 -1.2 626,873 + flgK OKGIIK_02700 0.76 -0.0 626,873 + flgK OKGIIK_02700 0.76 -0.7 626,873 + flgK OKGIIK_02700 0.76 -0.7 626,873 + flgK OKGIIK_02700 0.76 +0.5 626,873 + flgK OKGIIK_02700 0.76 -0.7 626,873 + flgK OKGIIK_02700 0.76 +0.6 626,873 + flgK OKGIIK_02700 0.76 -1.9 626,873 + flgK OKGIIK_02700 0.76 +0.1 626,873 + flgK OKGIIK_02700 0.76 +0.2 626,873 + flgK OKGIIK_02700 0.76 +0.1 626,874 - flgK OKGIIK_02700 0.76 +0.1 626,874 - flgK OKGIIK_02700 0.76 -0.0 626,874 - flgK OKGIIK_02700 0.76 +1.4 626,874 - flgK OKGIIK_02700 0.76 +1.5 626,874 - flgK OKGIIK_02700 0.76 -2.2 626,874 - flgK OKGIIK_02700 0.76 +0.3 626,874 - flgK OKGIIK_02700 0.76 -0.1 626,874 - flgK OKGIIK_02700 0.76 +0.7 626,985 + flgK OKGIIK_02700 0.82 -0.4 626,986 - flgK OKGIIK_02700 0.82 -0.0 626,986 - flgK OKGIIK_02700 0.82 +0.4 626,986 - flgK OKGIIK_02700 0.82 +1.3 626,986 - flgK OKGIIK_02700 0.82 -0.4 626,986 - flgK OKGIIK_02700 0.82 -1.6 626,986 - flgK OKGIIK_02700 0.82 +0.1 626,986 - flgK OKGIIK_02700 0.82 -1.0 626,986 - flgK OKGIIK_02700 0.82 +0.3 626,986 - flgK OKGIIK_02700 0.82 -0.3 626,986 - flgK OKGIIK_02700 0.82 +0.9 626,986 - flgK OKGIIK_02700 0.82 -1.1 627,167 + +1.0 627,167 + -0.3 627,167 + -0.1 627,167 + -0.7 627,168 - -0.8 627,168 - -0.3 627,168 - +0.6 627,168 - -1.7 627,168 - +0.1 627,168 - +1.6 627,254 + -0.1 627,254 + +0.9 627,254 + +0.4 627,254 + -0.4 627,254 + -1.8 627,255 - -0.9 627,255 - +0.7 627,326 + -1.1 627,326 + -0.1 627,412 + +1.5 627,412 + -0.0 627,412 + -0.0 627,412 + -1.4 627,412 + +0.9 627,412 + -0.0 627,412 + +0.8 627,413 - -1.9 627,413 - -2.1 627,413 - -0.1 627,413 - -0.4 627,515 + flgL OKGIIK_02705 0.15 +0.9 627,515 + flgL OKGIIK_02705 0.15 -0.4 627,516 - flgL OKGIIK_02705 0.15 -0.8 627,516 - flgL OKGIIK_02705 0.15 -0.1 627,731 + flgL OKGIIK_02705 0.33 +0.2 627,731 + flgL OKGIIK_02705 0.33 -0.7 627,731 + flgL OKGIIK_02705 0.33 -0.3 627,731 + flgL OKGIIK_02705 0.33 -0.6 627,731 + flgL OKGIIK_02705 0.33 +0.2 627,732 - flgL OKGIIK_02705 0.33 -0.4 627,732 - flgL OKGIIK_02705 0.33 +0.1 627,732 - flgL OKGIIK_02705 0.33 -0.8 627,732 - flgL OKGIIK_02705 0.33 +0.3 627,732 - flgL OKGIIK_02705 0.33 -0.9 627,732 - flgL OKGIIK_02705 0.33 -0.8 627,791 + flgL OKGIIK_02705 0.38 -1.4 627,791 + flgL OKGIIK_02705 0.38 +0.2 627,791 + flgL OKGIIK_02705 0.38 -0.5 627,791 + flgL OKGIIK_02705 0.38 -0.7 627,792 - flgL OKGIIK_02705 0.38 +0.9 627,792 - flgL OKGIIK_02705 0.38 -0.7 627,792 - flgL OKGIIK_02705 0.38 +0.6 627,792 - flgL OKGIIK_02705 0.38 +0.7 627,792 - flgL OKGIIK_02705 0.38 -0.9 628,005 - flgL OKGIIK_02705 0.56 +0.8 628,005 - flgL OKGIIK_02705 0.56 -0.9 628,005 - flgL OKGIIK_02705 0.56 +1.2 628,005 - flgL OKGIIK_02705 0.56 -1.7 628,034 + flgL OKGIIK_02705 0.58 +1.5 628,034 + flgL OKGIIK_02705 0.58 -0.1 628,035 - flgL OKGIIK_02705 0.58 +0.6 628,035 - flgL OKGIIK_02705 0.58 -1.0 628,035 - flgL OKGIIK_02705 0.58 +0.3 628,035 - flgL OKGIIK_02705 0.58 +0.7 628,035 - flgL OKGIIK_02705 0.58 -1.5 628,035 - flgL OKGIIK_02705 0.58 +0.3 628,079 + flgL OKGIIK_02705 0.62 -0.1 628,079 + flgL OKGIIK_02705 0.62 -1.0 628,079 + flgL OKGIIK_02705 0.62 +0.5 628,080 - flgL OKGIIK_02705 0.62 +0.2 628,080 - flgL OKGIIK_02705 0.62 -1.1 628,125 + flgL OKGIIK_02705 0.66 -1.0 628,125 + flgL OKGIIK_02705 0.66 -0.1 628,126 - flgL OKGIIK_02705 0.66 -0.1 628,126 - flgL OKGIIK_02705 0.66 -0.1 628,126 - flgL OKGIIK_02705 0.66 +0.2
Or see this region's nucleotide sequence