Experiment: R2A_PIPES with Cobalt chloride 1024 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_02620 and OKGIIK_02625 are separated by 108 nucleotides OKGIIK_02625 and flgM overlap by 4 nucleotides flgM and flgA are separated by 109 nucleotides flgA and OKGIIK_02640 overlap by 4 nucleotides
OKGIIK_02620: OKGIIK_02620 - Diguanylate phosphodiesterase, at 611,715 to 613,007
_02620
OKGIIK_02625: OKGIIK_02625 - Flagellar biosynthesis protein FlgN, at 613,116 to 613,562
_02625
OKGIIK_02630: flgM - flagellar biosynthesis anti-sigma factor FlgM, at 613,559 to 613,882
flgM
OKGIIK_02635: flgA - flagellar basal body P-ring formation protein FlgA, at 613,992 to 614,699
flgA
OKGIIK_02640: OKGIIK_02640 - Chemotaxis protein, at 614,696 to 616,000
_02640
Position (kb) 613
614 Strain fitness (log2 ratio) -2
-1
0
1
2 at 612.670 kb on + strand, within OKGIIK_02620 at 612.671 kb on - strand, within OKGIIK_02620 at 612.771 kb on + strand, within OKGIIK_02620 at 612.772 kb on - strand, within OKGIIK_02620 at 612.772 kb on - strand, within OKGIIK_02620 at 612.866 kb on - strand, within OKGIIK_02620 at 612.866 kb on - strand, within OKGIIK_02620 at 612.940 kb on + strand at 612.940 kb on + strand at 612.940 kb on + strand at 612.940 kb on + strand at 612.940 kb on + strand at 612.940 kb on + strand at 612.941 kb on - strand at 612.941 kb on - strand at 612.941 kb on - strand at 612.941 kb on - strand at 612.941 kb on - strand at 612.999 kb on + strand at 612.999 kb on + strand at 612.999 kb on + strand at 612.999 kb on + strand at 613.000 kb on - strand at 613.000 kb on - strand at 613.028 kb on + strand at 613.065 kb on + strand at 613.065 kb on + strand at 613.065 kb on + strand at 613.066 kb on - strand at 613.066 kb on - strand at 613.066 kb on - strand at 613.066 kb on - strand at 613.066 kb on - strand at 613.174 kb on + strand, within OKGIIK_02625 at 613.174 kb on + strand, within OKGIIK_02625 at 613.174 kb on + strand, within OKGIIK_02625 at 613.175 kb on - strand, within OKGIIK_02625 at 613.175 kb on - strand, within OKGIIK_02625 at 613.224 kb on + strand, within OKGIIK_02625 at 613.224 kb on + strand, within OKGIIK_02625 at 613.224 kb on + strand, within OKGIIK_02625 at 613.224 kb on + strand, within OKGIIK_02625 at 613.224 kb on + strand, within OKGIIK_02625 at 613.225 kb on - strand, within OKGIIK_02625 at 613.776 kb on - strand, within flgM at 613.913 kb on - strand at 613.913 kb on - strand at 613.913 kb on - strand at 613.945 kb on + strand at 613.945 kb on + strand at 613.945 kb on + strand at 613.945 kb on + strand at 613.945 kb on + strand at 613.946 kb on - strand at 613.946 kb on - strand at 613.946 kb on - strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.962 kb on + strand at 613.962 kb on + strand at 613.962 kb on + strand at 613.963 kb on - strand at 613.963 kb on - strand at 613.963 kb on - strand at 614.269 kb on + strand, within flgA at 614.269 kb on + strand, within flgA at 614.567 kb on - strand, within flgA at 614.567 kb on - strand, within flgA at 614.567 kb on - strand, within flgA at 614.567 kb on - strand, within flgA at 614.567 kb on - strand, within flgA
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 1024 uM remove 612,670 + OKGIIK_02620 0.74 -1.4 612,671 - OKGIIK_02620 0.74 -0.1 612,771 + OKGIIK_02620 0.82 -0.8 612,772 - OKGIIK_02620 0.82 -1.5 612,772 - OKGIIK_02620 0.82 +0.3 612,866 - OKGIIK_02620 0.89 +1.3 612,866 - OKGIIK_02620 0.89 -0.1 612,940 + -0.4 612,940 + -0.4 612,940 + -1.8 612,940 + -0.7 612,940 + +0.5 612,940 + -0.4 612,941 - -0.7 612,941 - -0.5 612,941 - -0.3 612,941 - +0.2 612,941 - +0.7 612,999 + +0.5 612,999 + -0.8 612,999 + -0.4 612,999 + +1.3 613,000 - +0.5 613,000 - -1.8 613,028 + -0.9 613,065 + -2.1 613,065 + -1.4 613,065 + -0.8 613,066 - +1.2 613,066 - +0.1 613,066 - +0.6 613,066 - -0.4 613,066 - -1.4 613,174 + OKGIIK_02625 0.13 +1.9 613,174 + OKGIIK_02625 0.13 +0.9 613,174 + OKGIIK_02625 0.13 -0.1 613,175 - OKGIIK_02625 0.13 +1.2 613,175 - OKGIIK_02625 0.13 -2.5 613,224 + OKGIIK_02625 0.24 +2.1 613,224 + OKGIIK_02625 0.24 -0.9 613,224 + OKGIIK_02625 0.24 -1.4 613,224 + OKGIIK_02625 0.24 -0.3 613,224 + OKGIIK_02625 0.24 +0.5 613,225 - OKGIIK_02625 0.24 +0.6 613,776 - flgM OKGIIK_02630 0.67 -2.6 613,913 - -0.4 613,913 - -1.1 613,913 - -1.1 613,945 + -0.8 613,945 + -0.2 613,945 + -1.5 613,945 + -0.6 613,945 + -1.9 613,946 - -0.4 613,946 - -1.2 613,946 - +0.3 613,960 + +1.6 613,960 + -1.9 613,960 + +0.6 613,960 + -1.3 613,960 + +0.1 613,960 + -0.1 613,960 + -0.7 613,960 + -2.2 613,960 + -1.3 613,960 + +0.6 613,960 + +0.3 613,960 + -0.1 613,960 + +1.3 613,960 + +0.3 613,960 + -0.7 613,960 + +1.1 613,960 + +0.0 613,960 + -0.5 613,960 + +1.1 613,960 + +0.4 613,960 + -0.6 613,961 - +0.9 613,961 - +0.0 613,961 - -0.4 613,961 - +0.0 613,961 - -0.8 613,961 - -0.4 613,961 - -1.7 613,961 - +0.1 613,961 - -0.7 613,961 - +1.5 613,961 - +1.1 613,961 - -0.4 613,961 - -0.1 613,961 - -1.6 613,961 - -0.3 613,961 - +1.6 613,961 - -0.4 613,962 + +0.6 613,962 + -0.9 613,962 + +0.4 613,963 - +0.1 613,963 - -1.1 613,963 - -0.5 614,269 + flgA OKGIIK_02635 0.39 +0.2 614,269 + flgA OKGIIK_02635 0.39 +0.9 614,567 - flgA OKGIIK_02635 0.81 +0.3 614,567 - flgA OKGIIK_02635 0.81 +1.3 614,567 - flgA OKGIIK_02635 0.81 +0.5 614,567 - flgA OKGIIK_02635 0.81 +0.4 614,567 - flgA OKGIIK_02635 0.81 -1.3
Or see this region's nucleotide sequence