Experiment: T2 MOI1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OHPLBJKB_01179 and OHPLBJKB_01180 are separated by 27 nucleotides OHPLBJKB_01180 and OHPLBJKB_01181 are separated by 16 nucleotides OHPLBJKB_01181 and OHPLBJKB_01182 are separated by 95 nucleotides OHPLBJKB_01182 and OHPLBJKB_01183 are separated by 16 nucleotides
OHPLBJKB_01179: OHPLBJKB_01179 - Cysteine desulfurase IscS, at 1,211,576 to 1,212,790
_01179
OHPLBJKB_01180: OHPLBJKB_01180 - Iron-sulfur cluster assembly scaffold protein IscU, at 1,212,818 to 1,213,204
_01180
OHPLBJKB_01181: OHPLBJKB_01181 - Iron-binding protein IscA, at 1,213,221 to 1,213,544
_01181
OHPLBJKB_01182: OHPLBJKB_01182 - Co-chaperone protein HscB, at 1,213,640 to 1,214,155
_01182
OHPLBJKB_01183: OHPLBJKB_01183 - Chaperone protein HscA, at 1,214,172 to 1,216,022
_01183
Position (kb) 1213
1214 Strain fitness (log2 ratio) -2
-1
0
1 at 1212.743 kb on + strand at 1213.180 kb on + strand at 1213.180 kb on + strand at 1213.202 kb on + strand at 1213.202 kb on + strand at 1213.258 kb on + strand, within OHPLBJKB_01181 at 1213.338 kb on + strand, within OHPLBJKB_01181 at 1213.484 kb on + strand, within OHPLBJKB_01181 at 1213.847 kb on + strand, within OHPLBJKB_01182 at 1214.104 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T2 MOI1 remove 1,212,743 + -0.4 1,213,180 + -0.2 1,213,180 + -1.9 1,213,202 + +0.0 1,213,202 + -0.6 1,213,258 + OHPLBJKB_01181 0.11 +0.4 1,213,338 + OHPLBJKB_01181 0.36 +1.5 1,213,484 + OHPLBJKB_01181 0.81 -1.2 1,213,847 + OHPLBJKB_01182 0.40 +0.4 1,214,104 + -1.1
Or see this region's nucleotide sequence