Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_28280 and NIAGMN_28285 are separated by 261 nucleotides NIAGMN_28285 and NIAGMN_28290 are separated by 224 nucleotides NIAGMN_28290 and rusA are separated by 62 nucleotides rusA and NIAGMN_28300 are separated by 12 nucleotides
NIAGMN_28280: NIAGMN_28280 - AraC family transcriptional regulator, at 5,471,431 to 5,472,018
_28280
NIAGMN_28285: NIAGMN_28285 - TrmB family transcriptional regulator, at 5,472,280 to 5,472,477
_28285
NIAGMN_28290: NIAGMN_28290 - antiterminator, at 5,472,702 to 5,473,256
_28290
NIAGMN_28295: rusA - Endodeoxyribonuclease RusA, at 5,473,319 to 5,473,624
rusA
NIAGMN_28300: NIAGMN_28300 - DUF968 domain-containing protein, at 5,473,637 to 5,474,686
_28300
Position (kb) 5472
5473
5474 Strain fitness (log2 ratio) -2
-1
0
1 at 5471.746 kb on + strand, within NIAGMN_28280 at 5471.830 kb on - strand, within NIAGMN_28280 at 5471.947 kb on - strand, within NIAGMN_28280 at 5472.861 kb on - strand, within NIAGMN_28290 at 5473.125 kb on - strand, within NIAGMN_28290 at 5473.260 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 5,471,746 + NIAGMN_28280 0.54 +0.6 5,471,830 - NIAGMN_28280 0.68 +0.4 5,471,947 - NIAGMN_28280 0.88 +0.2 5,472,861 - NIAGMN_28290 0.29 -2.6 5,473,125 - NIAGMN_28290 0.76 -0.2 5,473,260 + -0.0
Or see this region's nucleotide sequence