Strain Fitness in Escherichia coli ECRC102 around NIAGMN_28050

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmenH and NIAGMN_28050 are separated by 302 nucleotidesNIAGMN_28050 and tnpB are separated by 49 nucleotidestnpB and tnpB overlap by 4 nucleotidestnpB and tfaX are separated by 75 nucleotides NIAGMN_28045: menH - alpha/beta hydrolase, at 5,432,800 to 5,434,284 menH NIAGMN_28050: NIAGMN_28050 - IS66-like element ISEc8 family transposase, at 5,434,587 to 5,436,125 _28050 NIAGMN_28055: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 5,436,175 to 5,436,522 tnpB NIAGMN_28060: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 5,436,519 to 5,436,899 tnpB NIAGMN_28065: tfaX - Protein TfaX, at 5,436,975 to 5,437,223 tfaX Position (kb) 5434 5435 5436 5437Strain fitness (log2 ratio) -1 0 1at 5433.642 kb on - strand, within menHat 5437.004 kb on + strand, within tfaXat 5437.109 kb on + strand, within tfaX

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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5,433,642 - menH NIAGMN_28045 0.57 -0.5
5,437,004 + tfaX NIAGMN_28065 0.12 -0.2
5,437,109 + tfaX NIAGMN_28065 0.54 +0.8

Or see this region's nucleotide sequence