Strain Fitness in Escherichia coli ECRC102 around NIAGMN_27970

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntumuC and umuD overlap by 1 nucleotidesumuD and hlyE are separated by 372 nucleotideshlyE and ycgN are separated by 206 nucleotidesycgN and ycgM are separated by 91 nucleotides NIAGMN_27955: umuC - DNA polymerase V catalytic protein, at 5,419,849 to 5,421,117 umuC NIAGMN_27960: umuD - Protein UmuD, at 5,421,117 to 5,421,536 umuD NIAGMN_27970: hlyE - hemolysin HlyE, at 5,421,909 to 5,422,820 hlyE NIAGMN_27975: ycgN - YcgN family cysteine cluster protein, at 5,423,027 to 5,423,473 ycgN NIAGMN_27980: ycgM - fumarylacetoacetate hydrolase family protein, at 5,423,565 to 5,424,224 ycgM Position (kb) 5421 5422 5423Strain fitness (log2 ratio) -1 0 1at 5421.536 kb on + strandat 5422.764 kb on - strandat 5422.843 kb on - strandat 5423.176 kb on + strand, within ycgNat 5423.177 kb on - strand, within ycgNat 5423.286 kb on + strand, within ycgNat 5423.447 kb on - strandat 5423.506 kb on + strandat 5423.507 kb on - strandat 5423.507 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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5,421,536 + -0.1
5,422,764 - -0.3
5,422,843 - +0.3
5,423,176 + ycgN NIAGMN_27975 0.33 +0.7
5,423,177 - ycgN NIAGMN_27975 0.34 +1.1
5,423,286 + ycgN NIAGMN_27975 0.58 -0.1
5,423,447 - +0.5
5,423,506 + +0.7
5,423,507 - +1.2
5,423,507 - +0.7

Or see this region's nucleotide sequence