Strain Fitness in Escherichia coli ECRC102 around NIAGMN_27920

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntldcA and nhaP2 are separated by 95 nucleotidesnhaP2 and dadX are separated by 391 nucleotides NIAGMN_27915: ldcA - muramoyltetrapeptide carboxypeptidase, at 5,409,182 to 5,410,096 ldcA NIAGMN_27920: nhaP2 - potassium/proton antiporter, at 5,410,192 to 5,411,928 nhaP2 NIAGMN_27925: dadX - catabolic alanine racemase DadX, at 5,412,320 to 5,413,390 dadX Position (kb) 5410 5411 5412Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5409.198 kb on + strandat 5409.914 kb on + strand, within ldcAat 5410.226 kb on + strandat 5410.253 kb on + strandat 5410.257 kb on - strandat 5410.382 kb on + strand, within nhaP2at 5410.382 kb on + strand, within nhaP2at 5410.386 kb on + strand, within nhaP2at 5411.632 kb on - strand, within nhaP2at 5411.729 kb on + strand, within nhaP2at 5411.729 kb on + strand, within nhaP2at 5411.730 kb on - strand, within nhaP2at 5411.990 kb on + strandat 5412.619 kb on + strand, within dadXat 5412.621 kb on + strand, within dadXat 5412.622 kb on - strand, within dadXat 5412.673 kb on + strand, within dadX

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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5,409,198 + +0.2
5,409,914 + ldcA NIAGMN_27915 0.80 +1.6
5,410,226 + +0.3
5,410,253 + -0.1
5,410,257 - -1.5
5,410,382 + nhaP2 NIAGMN_27920 0.11 -0.8
5,410,382 + nhaP2 NIAGMN_27920 0.11 -0.4
5,410,386 + nhaP2 NIAGMN_27920 0.11 +1.2
5,411,632 - nhaP2 NIAGMN_27920 0.83 +1.1
5,411,729 + nhaP2 NIAGMN_27920 0.88 -0.6
5,411,729 + nhaP2 NIAGMN_27920 0.88 -3.9
5,411,730 - nhaP2 NIAGMN_27920 0.89 +0.1
5,411,990 + +1.2
5,412,619 + dadX NIAGMN_27925 0.28 -0.1
5,412,621 + dadX NIAGMN_27925 0.28 +1.0
5,412,622 - dadX NIAGMN_27925 0.28 +1.6
5,412,673 + dadX NIAGMN_27925 0.33 +0.2

Or see this region's nucleotide sequence