Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lapB and lapA are separated by 6 nucleotides lapA and pgpB are separated by 148 nucleotides pgpB and ribA are separated by 169 nucleotides ribA and acnA are separated by 63 nucleotides
NIAGMN_26725: lapB - lipopolysaccharide assembly protein LapB, at 5,204,077 to 5,205,246
lapB
NIAGMN_26730: lapA - lipopolysaccharide assembly protein LapA, at 5,205,253 to 5,205,561
lapA
NIAGMN_26735: pgpB - phosphatidylglycerophosphatase B, at 5,205,710 to 5,206,474
pgpB
NIAGMN_26740: ribA - GTP cyclohydrolase-2, at 5,206,644 to 5,207,234
ribA
NIAGMN_26745: acnA - aconitate hydratase AcnA, at 5,207,298 to 5,209,973
acnA
Position (kb) 5205
5206
5207 Strain fitness (log2 ratio) -1
0
1 at 5205.616 kb on - strand at 5205.629 kb on - strand at 5206.309 kb on + strand, within pgpB at 5206.362 kb on - strand, within pgpB at 5206.629 kb on + strand at 5206.629 kb on + strand at 5206.629 kb on + strand at 5207.232 kb on + strand at 5207.232 kb on + strand at 5207.250 kb on + strand at 5207.254 kb on + strand at 5207.299 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 5,205,616 - +0.2 5,205,629 - -0.8 5,206,309 + pgpB NIAGMN_26735 0.78 +0.5 5,206,362 - pgpB NIAGMN_26735 0.85 -1.4 5,206,629 + -0.1 5,206,629 + +0.2 5,206,629 + +1.2 5,207,232 + -0.5 5,207,232 + -1.1 5,207,250 + +1.2 5,207,254 + -0.4 5,207,299 + -0.1
Or see this region's nucleotide sequence