Strain Fitness in Escherichia coli ECRC102 around NIAGMN_26655

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsapD and sapF are separated by 1 nucleotidessapF and araJ are separated by 51 nucleotidesaraJ and tolC are separated by 8 nucleotides NIAGMN_26645: sapD - peptide ABC transporter ATP-binding protein SapD, at 5,184,950 to 5,185,942 sapD NIAGMN_26650: sapF - peptide ABC transporter ATP-binding protein SapF, at 5,185,944 to 5,186,750 sapF NIAGMN_26655: araJ - Bcr/CflA family drug resistance efflux transporter, at 5,186,802 to 5,187,965 araJ NIAGMN_26660: tolC - multidrug transporter, at 5,187,974 to 5,189,347 tolC Position (kb) 5186 5187 5188Strain fitness (log2 ratio) -1 0 1at 5187.093 kb on + strand, within araJat 5187.291 kb on - strand, within araJat 5187.292 kb on + strand, within araJat 5187.427 kb on - strand, within araJat 5187.447 kb on + strand, within araJat 5188.240 kb on + strand, within tolCat 5188.568 kb on + strand, within tolC

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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5,187,093 + araJ NIAGMN_26655 0.25 +1.2
5,187,291 - araJ NIAGMN_26655 0.42 +0.6
5,187,292 + araJ NIAGMN_26655 0.42 +0.8
5,187,427 - araJ NIAGMN_26655 0.54 +0.6
5,187,447 + araJ NIAGMN_26655 0.55 -0.6
5,188,240 + tolC NIAGMN_26660 0.19 +0.3
5,188,568 + tolC NIAGMN_26660 0.43 -0.1

Or see this region's nucleotide sequence