Strain Fitness in Escherichia coli ECRC102 around NIAGMN_24355

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcomR and bhsA are separated by 177 nucleotidesbhsA and ldtC are separated by 82 nucleotidesldtC and mfd are separated by 146 nucleotides NIAGMN_24345: comR - TetR family copper-responsive transcriptional repressor ComR, at 4,761,072 to 4,761,767 comR NIAGMN_24350: bhsA - multiple stress resistance protein BhsA, at 4,761,945 to 4,762,202 bhsA NIAGMN_24355: ldtC - L,D-transpeptidase LdtC, at 4,762,285 to 4,763,244 ldtC NIAGMN_24360: mfd - transcription-repair coupling factor, at 4,763,391 to 4,766,885 mfd Position (kb) 4762 4763 4764Strain fitness (log2 ratio) -2 -1 0 1at 4761.679 kb on - strand, within comRat 4762.865 kb on - strand, within ldtCat 4762.904 kb on + strand, within ldtCat 4763.552 kb on + strandat 4763.598 kb on - strandat 4764.106 kb on + strand, within mfd

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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4,761,679 - comR NIAGMN_24345 0.87 +1.6
4,762,865 - ldtC NIAGMN_24355 0.60 -2.0
4,762,904 + ldtC NIAGMN_24355 0.64 +0.7
4,763,552 + +0.7
4,763,598 - -0.9
4,764,106 + mfd NIAGMN_24360 0.20 -0.3

Or see this region's nucleotide sequence