Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_23930 and ymdE are separated by 13 nucleotides ymdE and ycdU overlap by 4 nucleotides ycdU and Ser_trna are separated by 192 nucleotides Ser_trna and ghrA are separated by 233 nucleotides
NIAGMN_23930: NIAGMN_23930 - hypothetical protein, at 4,686,928 to 4,688,316
_23930
NIAGMN_23935: ymdE - Putative uncharacterized protein YmdE, at 4,688,330 to 4,689,271
ymdE
NIAGMN_23940: ycdU - Uncharacterized protein YcdU, at 4,689,268 to 4,690,254
ycdU
NIAGMN_23945: Ser_trna - tRNA-Ser(gga), at 4,690,447 to 4,690,534
_trna
NIAGMN_23950: ghrA - glyoxylate/hydroxypyruvate reductase GhrA, at 4,690,768 to 4,691,706
ghrA
Position (kb)
4689
4690
4691 Strain fitness (log2 ratio)
-1
0
1
2 at 4688.290 kb on + strand at 4688.312 kb on + strand at 4688.312 kb on + strand at 4688.313 kb on - strand at 4688.313 kb on - strand at 4688.313 kb on - strand at 4688.813 kb on - strand, within ymdE at 4689.134 kb on - strand, within ymdE at 4689.167 kb on - strand, within ymdE at 4689.227 kb on - strand at 4689.328 kb on + strand at 4689.607 kb on + strand, within ycdU at 4690.285 kb on + strand at 4690.285 kb on + strand at 4690.288 kb on - strand at 4690.288 kb on - strand at 4690.401 kb on - strand at 4690.428 kb on - strand at 4690.441 kb on + strand at 4690.696 kb on + strand at 4690.696 kb on + strand at 4690.713 kb on + strand at 4690.720 kb on + strand at 4690.834 kb on - strand at 4691.072 kb on - strand, within ghrA at 4691.117 kb on - strand, within ghrA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 4,688,290 + -1.6 4,688,312 + +1.8 4,688,312 + -0.2 4,688,313 - +0.6 4,688,313 - +0.4 4,688,313 - +0.7 4,688,813 - ymdE NIAGMN_23935 0.51 -0.7 4,689,134 - ymdE NIAGMN_23935 0.85 -0.6 4,689,167 - ymdE NIAGMN_23935 0.89 +0.6 4,689,227 - +0.4 4,689,328 + +0.7 4,689,607 + ycdU NIAGMN_23940 0.34 +0.1 4,690,285 + +0.4 4,690,285 + -0.4 4,690,288 - +1.7 4,690,288 - -0.6 4,690,401 - -0.6 4,690,428 - -0.5 4,690,441 + -1.0 4,690,696 + +0.1 4,690,696 + +1.2 4,690,713 + +0.4 4,690,720 + -0.2 4,690,834 - +0.7 4,691,072 - ghrA NIAGMN_23950 0.32 +1.2 4,691,117 - ghrA NIAGMN_23950 0.37 -0.4
Or see this region's nucleotide sequence