Strain Fitness in Escherichia coli ECRC102 around NIAGMN_23070

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNIAGMN_23060 and NIAGMN_23065 overlap by 4 nucleotidesNIAGMN_23065 and appC are separated by 133 nucleotidesappC and appB are separated by 11 nucleotides NIAGMN_23060: NIAGMN_23060 - hydrogenase 1 maturation protease, at 4,536,779 to 4,537,177 _23060 NIAGMN_23065: NIAGMN_23065 - hydrogenase expression/formation protein, at 4,537,174 to 4,538,031 _23065 NIAGMN_23070: appC - cytochrome bd-II oxidase subunit 1, at 4,538,165 to 4,539,709 appC NIAGMN_23075: appB - cytochrome d ubiquinol oxidase subunit II, at 4,539,721 to 4,540,857 appB Position (kb) 4538 4539 4540Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4537.840 kb on + strand, within NIAGMN_23065at 4537.841 kb on - strand, within NIAGMN_23065at 4537.877 kb on + strand, within NIAGMN_23065at 4537.877 kb on + strand, within NIAGMN_23065at 4537.877 kb on + strand, within NIAGMN_23065at 4537.877 kb on + strand, within NIAGMN_23065at 4537.895 kb on + strand, within NIAGMN_23065at 4537.997 kb on + strandat 4537.997 kb on + strandat 4538.029 kb on + strandat 4538.059 kb on + strandat 4538.401 kb on + strand, within appCat 4538.423 kb on + strand, within appCat 4538.423 kb on + strand, within appCat 4538.423 kb on + strand, within appCat 4538.423 kb on + strand, within appCat 4538.535 kb on - strand, within appCat 4538.758 kb on + strand, within appCat 4538.759 kb on - strand, within appCat 4539.063 kb on + strand, within appCat 4539.130 kb on + strand, within appCat 4539.278 kb on + strand, within appCat 4539.650 kb on + strandat 4539.650 kb on + strandat 4539.650 kb on + strandat 4539.870 kb on + strand, within appBat 4539.870 kb on + strand, within appBat 4539.932 kb on + strand, within appBat 4539.955 kb on + strand, within appBat 4539.979 kb on + strand, within appBat 4539.979 kb on + strand, within appBat 4539.979 kb on + strand, within appBat 4540.229 kb on + strand, within appB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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4,537,840 + NIAGMN_23065 0.78 -0.2
4,537,841 - NIAGMN_23065 0.78 -0.3
4,537,877 + NIAGMN_23065 0.82 -0.5
4,537,877 + NIAGMN_23065 0.82 +0.6
4,537,877 + NIAGMN_23065 0.82 -0.6
4,537,877 + NIAGMN_23065 0.82 -0.3
4,537,895 + NIAGMN_23065 0.84 +3.0
4,537,997 + -2.1
4,537,997 + -1.1
4,538,029 + -0.2
4,538,059 + -0.4
4,538,401 + appC NIAGMN_23070 0.15 +0.4
4,538,423 + appC NIAGMN_23070 0.17 -0.5
4,538,423 + appC NIAGMN_23070 0.17 -1.5
4,538,423 + appC NIAGMN_23070 0.17 -0.3
4,538,423 + appC NIAGMN_23070 0.17 -1.2
4,538,535 - appC NIAGMN_23070 0.24 -2.2
4,538,758 + appC NIAGMN_23070 0.38 +0.8
4,538,759 - appC NIAGMN_23070 0.38 +1.4
4,539,063 + appC NIAGMN_23070 0.58 -1.2
4,539,130 + appC NIAGMN_23070 0.62 -1.9
4,539,278 + appC NIAGMN_23070 0.72 +1.2
4,539,650 + +0.2
4,539,650 + +0.8
4,539,650 + +0.7
4,539,870 + appB NIAGMN_23075 0.13 -1.4
4,539,870 + appB NIAGMN_23075 0.13 +0.5
4,539,932 + appB NIAGMN_23075 0.19 +1.4
4,539,955 + appB NIAGMN_23075 0.21 -0.4
4,539,979 + appB NIAGMN_23075 0.23 -0.6
4,539,979 + appB NIAGMN_23075 0.23 +0.7
4,539,979 + appB NIAGMN_23075 0.23 -0.2
4,540,229 + appB NIAGMN_23075 0.45 +0.6

Or see this region's nucleotide sequence