Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ada and alkB overlap by 1 nucleotides alkB and yojI are separated by 75 nucleotides yojI and mqo are separated by 217 nucleotides
NIAGMN_20975: ada - bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada, at 4,156,966 to 4,158,030
ada
NIAGMN_20980: alkB - DNA oxidative demethylase AlkB, at 4,158,030 to 4,158,680
alkB
NIAGMN_20985: yojI - microcin J25 efflux ABC transporter YojI, at 4,158,756 to 4,160,399
yojI
NIAGMN_20990: mqo - malate dehydrogenase (quinone), at 4,160,617 to 4,162,263
mqo
Position (kb) 4158
4159
4160
4161 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4157.833 kb on + strand, within ada at 4157.919 kb on - strand, within ada at 4157.919 kb on - strand, within ada at 4158.028 kb on + strand at 4158.127 kb on + strand, within alkB at 4158.128 kb on - strand, within alkB at 4158.194 kb on + strand, within alkB at 4158.194 kb on + strand, within alkB at 4158.194 kb on + strand, within alkB at 4158.194 kb on + strand, within alkB at 4158.194 kb on + strand, within alkB at 4158.194 kb on + strand, within alkB at 4158.194 kb on + strand, within alkB at 4158.194 kb on + strand, within alkB at 4158.194 kb on + strand, within alkB at 4158.195 kb on - strand, within alkB at 4158.195 kb on - strand, within alkB at 4158.195 kb on - strand, within alkB at 4158.195 kb on - strand, within alkB at 4158.195 kb on - strand, within alkB at 4158.255 kb on + strand, within alkB at 4158.513 kb on - strand, within alkB at 4158.692 kb on - strand at 4159.278 kb on - strand, within yojI at 4159.278 kb on - strand, within yojI at 4159.377 kb on + strand, within yojI at 4159.378 kb on - strand, within yojI at 4159.557 kb on + strand, within yojI at 4159.584 kb on - strand, within yojI at 4159.873 kb on - strand, within yojI at 4160.063 kb on + strand, within yojI at 4160.162 kb on + strand, within yojI at 4160.220 kb on + strand, within yojI at 4160.221 kb on - strand, within yojI at 4160.289 kb on + strand at 4160.289 kb on + strand at 4160.397 kb on + strand at 4160.398 kb on - strand at 4160.400 kb on + strand at 4160.446 kb on + strand at 4160.474 kb on - strand at 4160.611 kb on - strand at 4160.717 kb on + strand at 4160.717 kb on + strand at 4160.761 kb on + strand at 4160.762 kb on - strand at 4160.765 kb on + strand at 4161.019 kb on - strand, within mqo at 4161.019 kb on - strand, within mqo at 4161.261 kb on - strand, within mqo at 4161.285 kb on + strand, within mqo
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 4,157,833 + ada NIAGMN_20975 0.81 +0.1 4,157,919 - ada NIAGMN_20975 0.89 -0.9 4,157,919 - ada NIAGMN_20975 0.89 +0.4 4,158,028 + +0.9 4,158,127 + alkB NIAGMN_20980 0.15 +1.1 4,158,128 - alkB NIAGMN_20980 0.15 -0.4 4,158,194 + alkB NIAGMN_20980 0.25 +0.4 4,158,194 + alkB NIAGMN_20980 0.25 -0.9 4,158,194 + alkB NIAGMN_20980 0.25 -1.1 4,158,194 + alkB NIAGMN_20980 0.25 +1.6 4,158,194 + alkB NIAGMN_20980 0.25 +0.1 4,158,194 + alkB NIAGMN_20980 0.25 -0.3 4,158,194 + alkB NIAGMN_20980 0.25 +0.1 4,158,194 + alkB NIAGMN_20980 0.25 -0.6 4,158,194 + alkB NIAGMN_20980 0.25 -0.3 4,158,195 - alkB NIAGMN_20980 0.25 +1.0 4,158,195 - alkB NIAGMN_20980 0.25 +1.2 4,158,195 - alkB NIAGMN_20980 0.25 +0.8 4,158,195 - alkB NIAGMN_20980 0.25 +2.0 4,158,195 - alkB NIAGMN_20980 0.25 -0.6 4,158,255 + alkB NIAGMN_20980 0.35 +0.1 4,158,513 - alkB NIAGMN_20980 0.74 +0.4 4,158,692 - +0.6 4,159,278 - yojI NIAGMN_20985 0.32 +1.0 4,159,278 - yojI NIAGMN_20985 0.32 +0.4 4,159,377 + yojI NIAGMN_20985 0.38 -0.1 4,159,378 - yojI NIAGMN_20985 0.38 +0.4 4,159,557 + yojI NIAGMN_20985 0.49 +0.9 4,159,584 - yojI NIAGMN_20985 0.50 -0.9 4,159,873 - yojI NIAGMN_20985 0.68 +0.1 4,160,063 + yojI NIAGMN_20985 0.80 -0.3 4,160,162 + yojI NIAGMN_20985 0.86 -1.9 4,160,220 + yojI NIAGMN_20985 0.89 -0.6 4,160,221 - yojI NIAGMN_20985 0.89 +1.4 4,160,289 + -0.4 4,160,289 + -0.1 4,160,397 + +0.6 4,160,398 - +0.3 4,160,400 + +0.0 4,160,446 + -0.7 4,160,474 - +0.4 4,160,611 - -0.1 4,160,717 + -2.0 4,160,717 + +0.0 4,160,761 + +0.4 4,160,762 - +1.4 4,160,765 + +0.1 4,161,019 - mqo NIAGMN_20990 0.24 +3.0 4,161,019 - mqo NIAGMN_20990 0.24 -0.8 4,161,261 - mqo NIAGMN_20990 0.39 +0.4 4,161,285 + mqo NIAGMN_20990 0.41 +0.4
Or see this region's nucleotide sequence