Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfI and hyfH overlap by 4 nucleotides hyfH and hyfG are separated by 9 nucleotides hyfG and hyfF overlap by 11 nucleotides
NIAGMN_19630: hyfI - hydrogenase 4 catalytic subunit HyfI, at 3,874,064 to 3,874,822
hyfI
NIAGMN_19635: hyfH - hydrogenase 4 subunit H, at 3,874,819 to 3,875,364
hyfH
NIAGMN_19640: hyfG - hydrogenase 4 catalytic subunit HyfG, at 3,875,374 to 3,877,089
hyfG
NIAGMN_19645: hyfF - hydrogenase 4 subunit F, at 3,877,079 to 3,878,644
hyfF
Position (kb)
3875
3876
3877
3878 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3874.536 kb on + strand, within hyfI at 3874.537 kb on - strand, within hyfI at 3874.537 kb on - strand, within hyfI at 3874.555 kb on + strand, within hyfI at 3874.556 kb on - strand, within hyfI at 3874.583 kb on - strand, within hyfI at 3874.583 kb on - strand, within hyfI at 3874.583 kb on - strand, within hyfI at 3874.807 kb on + strand at 3874.808 kb on - strand at 3874.808 kb on - strand at 3874.822 kb on + strand at 3875.024 kb on - strand, within hyfH at 3875.082 kb on + strand, within hyfH at 3875.153 kb on + strand, within hyfH at 3875.197 kb on - strand, within hyfH at 3875.244 kb on + strand, within hyfH at 3875.244 kb on + strand, within hyfH at 3875.344 kb on - strand at 3875.375 kb on + strand at 3875.375 kb on + strand at 3875.426 kb on + strand at 3875.426 kb on + strand at 3875.427 kb on - strand at 3875.489 kb on + strand at 3875.552 kb on + strand, within hyfG at 3875.553 kb on - strand, within hyfG at 3875.577 kb on - strand, within hyfG at 3875.692 kb on - strand, within hyfG at 3875.693 kb on + strand, within hyfG at 3875.698 kb on - strand, within hyfG at 3875.698 kb on - strand, within hyfG at 3875.698 kb on - strand, within hyfG at 3875.824 kb on - strand, within hyfG at 3875.824 kb on - strand, within hyfG at 3875.824 kb on - strand, within hyfG at 3875.824 kb on - strand, within hyfG at 3875.924 kb on + strand, within hyfG at 3876.111 kb on - strand, within hyfG at 3876.180 kb on + strand, within hyfG at 3876.181 kb on - strand, within hyfG at 3876.241 kb on + strand, within hyfG at 3876.328 kb on - strand, within hyfG at 3876.330 kb on - strand, within hyfG at 3876.440 kb on + strand, within hyfG at 3876.440 kb on + strand, within hyfG at 3876.440 kb on + strand, within hyfG at 3876.441 kb on - strand, within hyfG at 3876.441 kb on - strand, within hyfG at 3876.519 kb on + strand, within hyfG at 3876.579 kb on - strand, within hyfG at 3876.579 kb on - strand, within hyfG at 3876.717 kb on - strand, within hyfG at 3876.720 kb on + strand, within hyfG at 3876.720 kb on + strand, within hyfG at 3876.720 kb on + strand, within hyfG at 3876.721 kb on - strand, within hyfG at 3876.721 kb on - strand, within hyfG at 3876.721 kb on - strand, within hyfG at 3876.876 kb on + strand, within hyfG at 3876.876 kb on + strand, within hyfG at 3876.876 kb on + strand, within hyfG at 3876.877 kb on - strand, within hyfG at 3876.877 kb on - strand, within hyfG at 3876.877 kb on - strand, within hyfG at 3876.877 kb on - strand, within hyfG at 3876.930 kb on - strand at 3876.933 kb on - strand at 3876.939 kb on - strand at 3876.944 kb on - strand at 3876.976 kb on - strand at 3877.081 kb on - strand at 3877.144 kb on + strand at 3877.144 kb on + strand at 3877.145 kb on - strand at 3877.181 kb on + strand at 3877.181 kb on + strand at 3877.181 kb on + strand at 3877.182 kb on - strand at 3877.182 kb on - strand at 3877.182 kb on - strand at 3877.182 kb on - strand at 3877.182 kb on - strand at 3877.184 kb on - strand at 3877.350 kb on - strand, within hyfF at 3877.383 kb on - strand, within hyfF at 3877.411 kb on + strand, within hyfF at 3877.411 kb on + strand, within hyfF at 3877.411 kb on + strand, within hyfF at 3877.412 kb on - strand, within hyfF at 3877.412 kb on - strand, within hyfF at 3877.412 kb on - strand, within hyfF at 3877.412 kb on - strand, within hyfF at 3877.439 kb on - strand, within hyfF at 3877.567 kb on - strand, within hyfF at 3877.577 kb on + strand, within hyfF at 3877.577 kb on + strand, within hyfF at 3877.577 kb on + strand, within hyfF at 3877.578 kb on - strand, within hyfF at 3877.578 kb on - strand, within hyfF at 3877.578 kb on - strand, within hyfF at 3877.625 kb on + strand, within hyfF at 3877.626 kb on - strand, within hyfF at 3877.729 kb on + strand, within hyfF at 3877.730 kb on - strand, within hyfF at 3877.835 kb on - strand, within hyfF at 3877.842 kb on + strand, within hyfF at 3877.842 kb on + strand, within hyfF at 3877.842 kb on + strand, within hyfF at 3877.842 kb on + strand, within hyfF at 3877.842 kb on + strand, within hyfF at 3877.843 kb on - strand, within hyfF at 3878.009 kb on + strand, within hyfF at 3878.010 kb on - strand, within hyfF
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 3,874,536 + hyfI NIAGMN_19630 0.62 +0.6 3,874,537 - hyfI NIAGMN_19630 0.62 +0.6 3,874,537 - hyfI NIAGMN_19630 0.62 -0.9 3,874,555 + hyfI NIAGMN_19630 0.65 -0.6 3,874,556 - hyfI NIAGMN_19630 0.65 -0.6 3,874,583 - hyfI NIAGMN_19630 0.68 +0.5 3,874,583 - hyfI NIAGMN_19630 0.68 +0.5 3,874,583 - hyfI NIAGMN_19630 0.68 -0.1 3,874,807 + +2.1 3,874,808 - +1.5 3,874,808 - -1.2 3,874,822 + -0.8 3,875,024 - hyfH NIAGMN_19635 0.38 +1.3 3,875,082 + hyfH NIAGMN_19635 0.48 +1.9 3,875,153 + hyfH NIAGMN_19635 0.61 +1.5 3,875,197 - hyfH NIAGMN_19635 0.69 +2.6 3,875,244 + hyfH NIAGMN_19635 0.78 +0.6 3,875,244 + hyfH NIAGMN_19635 0.78 -0.8 3,875,344 - +0.0 3,875,375 + +0.5 3,875,375 + +0.5 3,875,426 + +1.2 3,875,426 + +0.7 3,875,427 - +0.8 3,875,489 + +1.7 3,875,552 + hyfG NIAGMN_19640 0.10 -1.3 3,875,553 - hyfG NIAGMN_19640 0.10 +0.2 3,875,577 - hyfG NIAGMN_19640 0.12 -0.5 3,875,692 - hyfG NIAGMN_19640 0.19 -0.1 3,875,693 + hyfG NIAGMN_19640 0.19 +0.9 3,875,698 - hyfG NIAGMN_19640 0.19 -0.3 3,875,698 - hyfG NIAGMN_19640 0.19 +0.7 3,875,698 - hyfG NIAGMN_19640 0.19 +2.1 3,875,824 - hyfG NIAGMN_19640 0.26 +1.2 3,875,824 - hyfG NIAGMN_19640 0.26 +0.3 3,875,824 - hyfG NIAGMN_19640 0.26 -0.8 3,875,824 - hyfG NIAGMN_19640 0.26 +0.7 3,875,924 + hyfG NIAGMN_19640 0.32 +0.7 3,876,111 - hyfG NIAGMN_19640 0.43 +1.5 3,876,180 + hyfG NIAGMN_19640 0.47 -0.8 3,876,181 - hyfG NIAGMN_19640 0.47 +2.2 3,876,241 + hyfG NIAGMN_19640 0.51 +1.0 3,876,328 - hyfG NIAGMN_19640 0.56 +3.1 3,876,330 - hyfG NIAGMN_19640 0.56 +0.2 3,876,440 + hyfG NIAGMN_19640 0.62 +1.5 3,876,440 + hyfG NIAGMN_19640 0.62 -1.3 3,876,440 + hyfG NIAGMN_19640 0.62 -0.5 3,876,441 - hyfG NIAGMN_19640 0.62 +0.5 3,876,441 - hyfG NIAGMN_19640 0.62 -0.8 3,876,519 + hyfG NIAGMN_19640 0.67 +1.9 3,876,579 - hyfG NIAGMN_19640 0.70 -0.2 3,876,579 - hyfG NIAGMN_19640 0.70 +2.7 3,876,717 - hyfG NIAGMN_19640 0.78 +0.2 3,876,720 + hyfG NIAGMN_19640 0.78 +0.5 3,876,720 + hyfG NIAGMN_19640 0.78 -0.1 3,876,720 + hyfG NIAGMN_19640 0.78 -1.1 3,876,721 - hyfG NIAGMN_19640 0.78 -0.1 3,876,721 - hyfG NIAGMN_19640 0.78 +0.2 3,876,721 - hyfG NIAGMN_19640 0.78 +1.5 3,876,876 + hyfG NIAGMN_19640 0.88 -0.2 3,876,876 + hyfG NIAGMN_19640 0.88 -0.3 3,876,876 + hyfG NIAGMN_19640 0.88 -1.5 3,876,877 - hyfG NIAGMN_19640 0.88 +0.5 3,876,877 - hyfG NIAGMN_19640 0.88 +0.9 3,876,877 - hyfG NIAGMN_19640 0.88 +0.1 3,876,877 - hyfG NIAGMN_19640 0.88 +0.8 3,876,930 - -0.2 3,876,933 - -0.2 3,876,939 - -1.1 3,876,944 - +0.5 3,876,976 - +0.5 3,877,081 - -2.0 3,877,144 + +0.5 3,877,144 + -1.3 3,877,145 - +0.1 3,877,181 + +0.5 3,877,181 + -0.3 3,877,181 + +0.7 3,877,182 - -1.2 3,877,182 - +0.5 3,877,182 - +0.8 3,877,182 - +0.2 3,877,182 - +0.7 3,877,184 - -0.5 3,877,350 - hyfF NIAGMN_19645 0.17 -1.0 3,877,383 - hyfF NIAGMN_19645 0.19 -1.4 3,877,411 + hyfF NIAGMN_19645 0.21 +0.5 3,877,411 + hyfF NIAGMN_19645 0.21 -1.1 3,877,411 + hyfF NIAGMN_19645 0.21 +0.5 3,877,412 - hyfF NIAGMN_19645 0.21 -1.8 3,877,412 - hyfF NIAGMN_19645 0.21 -0.2 3,877,412 - hyfF NIAGMN_19645 0.21 -0.1 3,877,412 - hyfF NIAGMN_19645 0.21 +0.9 3,877,439 - hyfF NIAGMN_19645 0.23 -1.5 3,877,567 - hyfF NIAGMN_19645 0.31 +0.5 3,877,577 + hyfF NIAGMN_19645 0.32 +1.8 3,877,577 + hyfF NIAGMN_19645 0.32 -0.0 3,877,577 + hyfF NIAGMN_19645 0.32 -0.4 3,877,578 - hyfF NIAGMN_19645 0.32 +1.1 3,877,578 - hyfF NIAGMN_19645 0.32 -1.3 3,877,578 - hyfF NIAGMN_19645 0.32 -0.1 3,877,625 + hyfF NIAGMN_19645 0.35 +1.5 3,877,626 - hyfF NIAGMN_19645 0.35 -0.4 3,877,729 + hyfF NIAGMN_19645 0.42 -1.0 3,877,730 - hyfF NIAGMN_19645 0.42 -0.7 3,877,835 - hyfF NIAGMN_19645 0.48 +0.3 3,877,842 + hyfF NIAGMN_19645 0.49 +0.3 3,877,842 + hyfF NIAGMN_19645 0.49 +0.8 3,877,842 + hyfF NIAGMN_19645 0.49 +1.5 3,877,842 + hyfF NIAGMN_19645 0.49 -0.1 3,877,842 + hyfF NIAGMN_19645 0.49 +0.5 3,877,843 - hyfF NIAGMN_19645 0.49 -0.4 3,878,009 + hyfF NIAGMN_19645 0.59 -0.5 3,878,010 - hyfF NIAGMN_19645 0.59 -0.3
Or see this region's nucleotide sequence