Strain Fitness in Escherichia coli ECRC102 around NIAGMN_17595

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNIAGMN_17585 and NIAGMN_17590 are separated by 13 nucleotidesNIAGMN_17590 and sctI are separated by 19 nucleotidessctI and escJ overlap by 4 nucleotidesescJ and NIAGMN_17605 are separated by 15 nucleotides NIAGMN_17585: NIAGMN_17585 - Type III secretion protein EprH, at 3,480,456 to 3,481,190 _17585 NIAGMN_17590: NIAGMN_17590 - type III secretion system needle complex protein, at 3,481,204 to 3,481,443 _17590 NIAGMN_17595: sctI - type III secretion system inner rod subunit SctI, at 3,481,463 to 3,481,795 sctI NIAGMN_17600: escJ - EscJ/YscJ/HrcJ family type III secretion inner membrane ring protein, at 3,481,792 to 3,482,526 escJ NIAGMN_17605: NIAGMN_17605 - type III secretion apparatus protein OrgA/MxiK, at 3,482,542 to 3,483,123 _17605 Position (kb) 3481 3482Strain fitness (log2 ratio) -1 0 1 2at 3481.363 kb on - strand, within NIAGMN_17590at 3481.607 kb on + strand, within sctIat 3481.607 kb on + strand, within sctIat 3481.608 kb on - strand, within sctIat 3481.799 kb on - strandat 3481.808 kb on + strandat 3481.911 kb on + strand, within escJat 3481.912 kb on - strand, within escJat 3481.960 kb on + strand, within escJat 3482.567 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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3,481,363 - NIAGMN_17590 0.66 -0.6
3,481,607 + sctI NIAGMN_17595 0.43 -0.2
3,481,607 + sctI NIAGMN_17595 0.43 +0.0
3,481,608 - sctI NIAGMN_17595 0.44 -1.7
3,481,799 - -1.0
3,481,808 + -0.3
3,481,911 + escJ NIAGMN_17600 0.16 +0.6
3,481,912 - escJ NIAGMN_17600 0.16 -1.1
3,481,960 + escJ NIAGMN_17600 0.23 +2.6
3,482,567 + +0.6

Or see this region's nucleotide sequence