Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_17585 and NIAGMN_17590 are separated by 13 nucleotides NIAGMN_17590 and sctI are separated by 19 nucleotides sctI and escJ overlap by 4 nucleotides escJ and NIAGMN_17605 are separated by 15 nucleotides
NIAGMN_17585: NIAGMN_17585 - Type III secretion protein EprH, at 3,480,456 to 3,481,190
_17585
NIAGMN_17590: NIAGMN_17590 - type III secretion system needle complex protein, at 3,481,204 to 3,481,443
_17590
NIAGMN_17595: sctI - type III secretion system inner rod subunit SctI, at 3,481,463 to 3,481,795
sctI
NIAGMN_17600: escJ - EscJ/YscJ/HrcJ family type III secretion inner membrane ring protein, at 3,481,792 to 3,482,526
escJ
NIAGMN_17605: NIAGMN_17605 - type III secretion apparatus protein OrgA/MxiK, at 3,482,542 to 3,483,123
_17605
Position (kb)
3481
3482 Strain fitness (log2 ratio)
-1
0
1
2 at 3481.363 kb on - strand, within NIAGMN_17590 at 3481.607 kb on + strand, within sctI at 3481.607 kb on + strand, within sctI at 3481.608 kb on - strand, within sctI at 3481.799 kb on - strand at 3481.808 kb on + strand at 3481.911 kb on + strand, within escJ at 3481.912 kb on - strand, within escJ at 3481.960 kb on + strand, within escJ at 3482.567 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 3,481,363 - NIAGMN_17590 0.66 -0.6 3,481,607 + sctI NIAGMN_17595 0.43 -0.2 3,481,607 + sctI NIAGMN_17595 0.43 +0.0 3,481,608 - sctI NIAGMN_17595 0.44 -1.7 3,481,799 - -1.0 3,481,808 + -0.3 3,481,911 + escJ NIAGMN_17600 0.16 +0.6 3,481,912 - escJ NIAGMN_17600 0.16 -1.1 3,481,960 + escJ NIAGMN_17600 0.23 +2.6 3,482,567 + +0.6
Or see this region's nucleotide sequence