Strain Fitness in Escherichia coli ECRC102 around NIAGMN_15865

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfMet_trna and rimP are separated by 206 nucleotidesrimP and nusA are separated by 27 nucleotidesnusA and infB are separated by 24 nucleotides NIAGMN_15855: fMet_trna - tRNA-fMet(cat), at 3,140,942 to 3,141,018 _trna NIAGMN_15860: rimP - ribosome maturation factor RimP, at 3,141,225 to 3,141,677 rimP NIAGMN_15865: nusA - Transcription termination/antitermination protein NusA, at 3,141,705 to 3,143,192 nusA NIAGMN_15870: infB - Translation initiation factor IF-2, at 3,143,217 to 3,145,889 infB Position (kb) 3141 3142 3143 3144Strain fitness (log2 ratio) -2 -1 0 1at 3140.713 kb on - strandat 3140.732 kb on - strandat 3140.732 kb on - strandat 3140.741 kb on - strandat 3140.809 kb on - strandat 3140.844 kb on + strandat 3140.845 kb on - strandat 3140.847 kb on - strandat 3140.895 kb on + strandat 3141.137 kb on + strandat 3141.139 kb on + strandat 3142.742 kb on + strand, within nusAat 3143.023 kb on + strand, within nusAat 3143.023 kb on + strand, within nusAat 3143.023 kb on + strand, within nusAat 3143.066 kb on + strandat 3143.066 kb on + strandat 3143.165 kb on + strandat 3143.165 kb on + strandat 3143.165 kb on + strandat 3143.165 kb on + strandat 3143.190 kb on + strandat 3143.193 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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3,140,713 - -1.3
3,140,732 - +1.7
3,140,732 - +1.7
3,140,741 - +1.3
3,140,809 - +0.7
3,140,844 + +1.3
3,140,845 - -0.5
3,140,847 - +1.0
3,140,895 + +0.4
3,141,137 + -0.9
3,141,139 + +0.5
3,142,742 + nusA NIAGMN_15865 0.70 +0.7
3,143,023 + nusA NIAGMN_15865 0.89 -1.0
3,143,023 + nusA NIAGMN_15865 0.89 -1.3
3,143,023 + nusA NIAGMN_15865 0.89 -0.4
3,143,066 + -0.6
3,143,066 + -0.3
3,143,165 + +0.7
3,143,165 + +0.5
3,143,165 + -0.4
3,143,165 + -0.5
3,143,190 + -0.1
3,143,193 + -1.8

Or see this region's nucleotide sequence