Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhgF and greB are separated by 96 nucleotides greB and ompR_G63V are separated by 227 nucleotides ompR_G63V and envZ overlap by 4 nucleotides
NIAGMN_14795: yhgF - Protein YhgF, at 2,949,829 to 2,952,150
yhgF
NIAGMN_14800: greB - transcription elongation factor GreB, at 2,952,247 to 2,952,723
greB
NIAGMN_14805: ompR_G63V - two-component system response regulator OmpR, at 2,952,951 to 2,953,670
_G63V
NIAGMN_14810: envZ - two-component system sensor histidine kinase EnvZ, at 2,953,667 to 2,955,019
envZ
Position (kb)
2952
2953
2954 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2951.967 kb on - strand at 2951.988 kb on + strand at 2952.042 kb on + strand at 2952.043 kb on - strand at 2952.055 kb on - strand at 2952.055 kb on - strand at 2952.188 kb on + strand at 2952.188 kb on + strand at 2952.188 kb on + strand at 2952.188 kb on + strand at 2952.188 kb on + strand at 2952.189 kb on - strand at 2952.189 kb on - strand at 2952.189 kb on - strand at 2952.189 kb on - strand at 2952.189 kb on - strand at 2952.189 kb on - strand at 2952.192 kb on + strand at 2952.192 kb on + strand at 2952.192 kb on + strand at 2952.192 kb on + strand at 2952.193 kb on - strand at 2952.236 kb on - strand at 2952.260 kb on + strand at 2952.261 kb on - strand at 2952.261 kb on - strand at 2952.267 kb on + strand at 2952.268 kb on - strand at 2952.273 kb on + strand at 2952.273 kb on + strand at 2952.273 kb on + strand at 2952.274 kb on - strand at 2952.278 kb on + strand at 2952.278 kb on + strand at 2952.278 kb on + strand at 2952.278 kb on + strand at 2952.279 kb on - strand at 2952.279 kb on - strand at 2952.279 kb on - strand at 2952.279 kb on - strand at 2952.279 kb on - strand at 2952.303 kb on + strand, within greB at 2952.303 kb on + strand, within greB at 2952.304 kb on - strand, within greB at 2952.374 kb on + strand, within greB at 2952.374 kb on + strand, within greB at 2952.375 kb on - strand, within greB at 2952.375 kb on - strand, within greB at 2952.375 kb on - strand, within greB at 2952.375 kb on - strand, within greB at 2952.381 kb on + strand, within greB at 2952.382 kb on - strand, within greB at 2952.411 kb on + strand, within greB at 2952.495 kb on - strand, within greB at 2952.525 kb on + strand, within greB at 2952.526 kb on - strand, within greB at 2952.526 kb on - strand, within greB at 2952.533 kb on + strand, within greB at 2952.533 kb on + strand, within greB at 2952.534 kb on - strand, within greB at 2952.570 kb on + strand, within greB at 2952.571 kb on - strand, within greB at 2952.574 kb on - strand, within greB at 2952.574 kb on - strand, within greB at 2952.575 kb on + strand, within greB at 2952.575 kb on + strand, within greB at 2952.575 kb on + strand, within greB at 2952.575 kb on + strand, within greB at 2952.576 kb on - strand, within greB at 2952.576 kb on - strand, within greB at 2952.576 kb on - strand, within greB at 2952.576 kb on - strand, within greB at 2952.577 kb on - strand, within greB at 2952.582 kb on - strand, within greB at 2952.665 kb on + strand, within greB at 2952.665 kb on + strand, within greB at 2952.665 kb on + strand, within greB at 2952.666 kb on - strand, within greB at 2952.666 kb on - strand, within greB at 2952.666 kb on - strand, within greB at 2952.666 kb on - strand, within greB at 2952.666 kb on - strand, within greB at 2952.666 kb on - strand, within greB at 2952.669 kb on + strand, within greB at 2952.669 kb on + strand, within greB at 2952.669 kb on + strand, within greB at 2952.669 kb on + strand, within greB at 2952.669 kb on + strand, within greB at 2952.670 kb on - strand, within greB at 2952.670 kb on - strand, within greB at 2952.750 kb on + strand at 2952.766 kb on + strand at 2952.767 kb on - strand at 2952.767 kb on - strand at 2952.767 kb on - strand at 2952.767 kb on - strand at 2952.768 kb on + strand at 2952.769 kb on - strand at 2952.769 kb on - strand at 2952.769 kb on - strand at 2952.778 kb on + strand at 2952.779 kb on + strand at 2952.779 kb on + strand at 2952.779 kb on + strand at 2952.780 kb on - strand at 2952.816 kb on + strand at 2952.816 kb on + strand at 2952.822 kb on + strand at 2952.823 kb on - strand at 2952.838 kb on - strand at 2952.877 kb on + strand at 2952.903 kb on + strand at 2952.909 kb on + strand at 2952.909 kb on + strand at 2952.910 kb on - strand at 2952.917 kb on + strand at 2952.917 kb on + strand at 2952.917 kb on + strand at 2952.943 kb on + strand at 2952.963 kb on + strand at 2952.964 kb on - strand at 2953.017 kb on + strand at 2953.017 kb on + strand at 2953.103 kb on + strand, within ompR_G63V at 2953.103 kb on + strand, within ompR_G63V at 2953.103 kb on + strand, within ompR_G63V at 2953.104 kb on - strand, within ompR_G63V at 2953.118 kb on - strand, within ompR_G63V at 2953.123 kb on + strand, within ompR_G63V at 2953.123 kb on + strand, within ompR_G63V at 2953.123 kb on + strand, within ompR_G63V at 2953.254 kb on + strand, within ompR_G63V at 2953.360 kb on + strand, within ompR_G63V at 2953.638 kb on + strand at 2953.639 kb on - strand at 2953.639 kb on - strand at 2953.662 kb on - strand at 2953.708 kb on + strand at 2953.708 kb on + strand at 2953.713 kb on + strand at 2953.713 kb on + strand at 2953.713 kb on + strand at 2953.714 kb on - strand at 2953.757 kb on + strand at 2953.757 kb on + strand at 2953.757 kb on + strand at 2953.758 kb on - strand at 2953.758 kb on - strand at 2953.804 kb on + strand, within envZ at 2953.807 kb on + strand, within envZ at 2953.831 kb on + strand, within envZ at 2953.832 kb on - strand, within envZ at 2953.832 kb on - strand, within envZ at 2953.832 kb on - strand, within envZ at 2953.832 kb on - strand, within envZ at 2953.908 kb on - strand, within envZ at 2953.908 kb on - strand, within envZ at 2953.931 kb on + strand, within envZ at 2953.979 kb on + strand, within envZ at 2953.980 kb on - strand, within envZ at 2953.990 kb on - strand, within envZ at 2954.092 kb on + strand, within envZ at 2954.093 kb on - strand, within envZ at 2954.153 kb on + strand, within envZ at 2954.154 kb on - strand, within envZ at 2954.155 kb on + strand, within envZ at 2954.155 kb on + strand, within envZ at 2954.155 kb on + strand, within envZ at 2954.155 kb on + strand, within envZ at 2954.155 kb on + strand, within envZ at 2954.156 kb on - strand, within envZ at 2954.156 kb on - strand, within envZ at 2954.156 kb on - strand, within envZ at 2954.167 kb on + strand, within envZ at 2954.167 kb on + strand, within envZ at 2954.167 kb on + strand, within envZ at 2954.257 kb on + strand, within envZ at 2954.258 kb on - strand, within envZ at 2954.266 kb on + strand, within envZ at 2954.288 kb on + strand, within envZ at 2954.289 kb on - strand, within envZ at 2954.289 kb on - strand, within envZ at 2954.332 kb on + strand, within envZ at 2954.333 kb on - strand, within envZ at 2954.348 kb on - strand, within envZ at 2954.404 kb on + strand, within envZ at 2954.420 kb on - strand, within envZ at 2954.460 kb on - strand, within envZ at 2954.479 kb on + strand, within envZ at 2954.479 kb on + strand, within envZ at 2954.480 kb on - strand, within envZ at 2954.485 kb on + strand, within envZ at 2954.519 kb on - strand, within envZ at 2954.577 kb on + strand, within envZ at 2954.590 kb on + strand, within envZ
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 2,951,967 - +0.3 2,951,988 + +1.3 2,952,042 + -0.2 2,952,043 - +1.7 2,952,055 - +0.2 2,952,055 - +0.4 2,952,188 + -0.5 2,952,188 + -1.8 2,952,188 + -0.2 2,952,188 + -2.4 2,952,188 + +0.2 2,952,189 - -0.4 2,952,189 - -0.3 2,952,189 - +0.2 2,952,189 - -0.2 2,952,189 - -0.2 2,952,189 - -0.8 2,952,192 + -0.2 2,952,192 + +0.5 2,952,192 + +0.1 2,952,192 + +0.5 2,952,193 - +0.8 2,952,236 - +0.6 2,952,260 + +0.5 2,952,261 - +0.2 2,952,261 - +0.7 2,952,267 + +1.0 2,952,268 - +1.3 2,952,273 + -2.4 2,952,273 + +2.4 2,952,273 + -0.7 2,952,274 - -1.9 2,952,278 + +0.9 2,952,278 + -0.5 2,952,278 + -1.3 2,952,278 + -0.3 2,952,279 - +0.2 2,952,279 - -0.5 2,952,279 - +0.3 2,952,279 - +1.4 2,952,279 - -0.0 2,952,303 + greB NIAGMN_14800 0.12 -0.1 2,952,303 + greB NIAGMN_14800 0.12 -0.6 2,952,304 - greB NIAGMN_14800 0.12 -1.9 2,952,374 + greB NIAGMN_14800 0.27 -0.4 2,952,374 + greB NIAGMN_14800 0.27 +0.7 2,952,375 - greB NIAGMN_14800 0.27 +0.7 2,952,375 - greB NIAGMN_14800 0.27 -0.3 2,952,375 - greB NIAGMN_14800 0.27 -0.3 2,952,375 - greB NIAGMN_14800 0.27 +0.9 2,952,381 + greB NIAGMN_14800 0.28 +0.1 2,952,382 - greB NIAGMN_14800 0.28 +0.7 2,952,411 + greB NIAGMN_14800 0.34 -0.9 2,952,495 - greB NIAGMN_14800 0.52 -0.5 2,952,525 + greB NIAGMN_14800 0.58 -1.1 2,952,526 - greB NIAGMN_14800 0.58 +0.3 2,952,526 - greB NIAGMN_14800 0.58 +0.9 2,952,533 + greB NIAGMN_14800 0.60 +1.7 2,952,533 + greB NIAGMN_14800 0.60 +1.7 2,952,534 - greB NIAGMN_14800 0.60 -1.2 2,952,570 + greB NIAGMN_14800 0.68 -0.8 2,952,571 - greB NIAGMN_14800 0.68 -0.3 2,952,574 - greB NIAGMN_14800 0.69 -0.3 2,952,574 - greB NIAGMN_14800 0.69 +0.1 2,952,575 + greB NIAGMN_14800 0.69 +0.8 2,952,575 + greB NIAGMN_14800 0.69 +0.1 2,952,575 + greB NIAGMN_14800 0.69 +0.5 2,952,575 + greB NIAGMN_14800 0.69 -0.8 2,952,576 - greB NIAGMN_14800 0.69 -0.3 2,952,576 - greB NIAGMN_14800 0.69 -0.4 2,952,576 - greB NIAGMN_14800 0.69 -0.3 2,952,576 - greB NIAGMN_14800 0.69 +0.5 2,952,577 - greB NIAGMN_14800 0.69 -0.3 2,952,582 - greB NIAGMN_14800 0.70 -0.5 2,952,665 + greB NIAGMN_14800 0.88 -0.6 2,952,665 + greB NIAGMN_14800 0.88 -1.1 2,952,665 + greB NIAGMN_14800 0.88 -0.3 2,952,666 - greB NIAGMN_14800 0.88 +0.4 2,952,666 - greB NIAGMN_14800 0.88 -0.3 2,952,666 - greB NIAGMN_14800 0.88 +0.4 2,952,666 - greB NIAGMN_14800 0.88 +0.8 2,952,666 - greB NIAGMN_14800 0.88 +0.3 2,952,666 - greB NIAGMN_14800 0.88 +1.7 2,952,669 + greB NIAGMN_14800 0.88 +0.3 2,952,669 + greB NIAGMN_14800 0.88 +0.1 2,952,669 + greB NIAGMN_14800 0.88 +0.3 2,952,669 + greB NIAGMN_14800 0.88 +0.1 2,952,669 + greB NIAGMN_14800 0.88 +0.3 2,952,670 - greB NIAGMN_14800 0.89 -0.2 2,952,670 - greB NIAGMN_14800 0.89 +0.4 2,952,750 + +1.0 2,952,766 + +0.5 2,952,767 - -0.4 2,952,767 - -0.1 2,952,767 - +1.4 2,952,767 - +0.7 2,952,768 + +1.7 2,952,769 - -0.1 2,952,769 - +0.0 2,952,769 - +0.6 2,952,778 + +0.4 2,952,779 + +0.3 2,952,779 + -0.8 2,952,779 + +1.7 2,952,780 - +0.4 2,952,816 + +0.7 2,952,816 + -0.2 2,952,822 + -0.1 2,952,823 - -1.5 2,952,838 - +2.3 2,952,877 + -0.5 2,952,903 + +1.2 2,952,909 + -1.8 2,952,909 + +0.1 2,952,910 - +0.5 2,952,917 + +0.4 2,952,917 + +0.8 2,952,917 + -2.2 2,952,943 + +0.7 2,952,963 + +3.3 2,952,964 - +2.7 2,953,017 + +1.7 2,953,017 + +0.4 2,953,103 + ompR_G63V NIAGMN_14805 0.21 +0.3 2,953,103 + ompR_G63V NIAGMN_14805 0.21 -0.3 2,953,103 + ompR_G63V NIAGMN_14805 0.21 -0.2 2,953,104 - ompR_G63V NIAGMN_14805 0.21 +0.7 2,953,118 - ompR_G63V NIAGMN_14805 0.23 -0.9 2,953,123 + ompR_G63V NIAGMN_14805 0.24 +0.2 2,953,123 + ompR_G63V NIAGMN_14805 0.24 +0.1 2,953,123 + ompR_G63V NIAGMN_14805 0.24 +1.3 2,953,254 + ompR_G63V NIAGMN_14805 0.42 +0.1 2,953,360 + ompR_G63V NIAGMN_14805 0.57 +1.4 2,953,638 + +1.7 2,953,639 - +0.7 2,953,639 - +0.9 2,953,662 - +0.1 2,953,708 + +2.1 2,953,708 + +0.4 2,953,713 + +1.7 2,953,713 + +2.3 2,953,713 + +1.0 2,953,714 - -1.8 2,953,757 + +0.7 2,953,757 + +0.1 2,953,757 + +0.6 2,953,758 - +0.2 2,953,758 - +2.7 2,953,804 + envZ NIAGMN_14810 0.10 +0.1 2,953,807 + envZ NIAGMN_14810 0.10 +0.7 2,953,831 + envZ NIAGMN_14810 0.12 -0.6 2,953,832 - envZ NIAGMN_14810 0.12 -1.1 2,953,832 - envZ NIAGMN_14810 0.12 -1.5 2,953,832 - envZ NIAGMN_14810 0.12 -0.5 2,953,832 - envZ NIAGMN_14810 0.12 +1.7 2,953,908 - envZ NIAGMN_14810 0.18 -0.6 2,953,908 - envZ NIAGMN_14810 0.18 +1.2 2,953,931 + envZ NIAGMN_14810 0.20 +0.7 2,953,979 + envZ NIAGMN_14810 0.23 +0.4 2,953,980 - envZ NIAGMN_14810 0.23 +1.7 2,953,990 - envZ NIAGMN_14810 0.24 +0.4 2,954,092 + envZ NIAGMN_14810 0.31 -1.0 2,954,093 - envZ NIAGMN_14810 0.31 +1.0 2,954,153 + envZ NIAGMN_14810 0.36 +0.3 2,954,154 - envZ NIAGMN_14810 0.36 +2.3 2,954,155 + envZ NIAGMN_14810 0.36 +1.1 2,954,155 + envZ NIAGMN_14810 0.36 -0.8 2,954,155 + envZ NIAGMN_14810 0.36 -0.6 2,954,155 + envZ NIAGMN_14810 0.36 -0.9 2,954,155 + envZ NIAGMN_14810 0.36 +0.7 2,954,156 - envZ NIAGMN_14810 0.36 +1.7 2,954,156 - envZ NIAGMN_14810 0.36 -0.1 2,954,156 - envZ NIAGMN_14810 0.36 +0.5 2,954,167 + envZ NIAGMN_14810 0.37 +0.1 2,954,167 + envZ NIAGMN_14810 0.37 +0.7 2,954,167 + envZ NIAGMN_14810 0.37 +1.1 2,954,257 + envZ NIAGMN_14810 0.44 +0.7 2,954,258 - envZ NIAGMN_14810 0.44 +1.7 2,954,266 + envZ NIAGMN_14810 0.44 -0.3 2,954,288 + envZ NIAGMN_14810 0.46 +0.6 2,954,289 - envZ NIAGMN_14810 0.46 -0.1 2,954,289 - envZ NIAGMN_14810 0.46 -0.6 2,954,332 + envZ NIAGMN_14810 0.49 +0.3 2,954,333 - envZ NIAGMN_14810 0.49 +0.3 2,954,348 - envZ NIAGMN_14810 0.50 +0.5 2,954,404 + envZ NIAGMN_14810 0.54 -1.1 2,954,420 - envZ NIAGMN_14810 0.56 -0.3 2,954,460 - envZ NIAGMN_14810 0.59 +0.3 2,954,479 + envZ NIAGMN_14810 0.60 +0.1 2,954,479 + envZ NIAGMN_14810 0.60 +1.9 2,954,480 - envZ NIAGMN_14810 0.60 +1.1 2,954,485 + envZ NIAGMN_14810 0.60 -0.3 2,954,519 - envZ NIAGMN_14810 0.63 +0.1 2,954,577 + envZ NIAGMN_14810 0.67 +0.4 2,954,590 + envZ NIAGMN_14810 0.68 +0.1
Or see this region's nucleotide sequence