Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nusG and secE are separated by 1 nucleotides secE and tuf are separated by 229 nucleotides tuf and Thr_trna are separated by 114 nucleotides Thr_trna and Gly_trna are separated by 6 nucleotides Gly_trna and Tyr_trna are separated by 116 nucleotides Tyr_trna and Thr_trna are separated by 8 nucleotides Thr_trna and coaA are separated by 385 nucleotides
NIAGMN_11200: nusG - transcription termination/antitermination protein NusG, at 2,214,419 to 2,214,964
nusG
NIAGMN_11205: secE - preprotein translocase subunit SecE, at 2,214,966 to 2,215,349
secE
NIAGMN_11210: tuf - elongation factor Tu, at 2,215,579 to 2,216,763
tuf
NIAGMN_11215: Thr_trna - tRNA-Thr(ggt), at 2,216,878 to 2,216,953
_trna
NIAGMN_11220: Gly_trna - tRNA-Gly(tcc), at 2,216,960 to 2,217,034
_trna
NIAGMN_11225: Tyr_trna - tRNA-Tyr(gta), at 2,217,151 to 2,217,235
_trna
NIAGMN_11230: Thr_trna - tRNA-Thr(tgt), at 2,217,244 to 2,217,319
_trna
NIAGMN_11235: coaA - type I pantothenate kinase, at 2,217,705 to 2,218,631
coaA
Position (kb)
2215
2216
2217 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2215.759 kb on - strand, within tuf at 2216.169 kb on - strand, within tuf at 2216.169 kb on - strand, within tuf at 2216.777 kb on - strand at 2216.788 kb on - strand at 2216.844 kb on - strand at 2216.844 kb on - strand at 2216.918 kb on - strand, within Thr_trna at 2217.117 kb on - strand at 2217.117 kb on - strand at 2217.130 kb on - strand at 2217.200 kb on - strand, within Tyr_trna at 2217.440 kb on + strand at 2217.441 kb on - strand at 2217.441 kb on - strand at 2217.486 kb on - strand at 2217.492 kb on + strand at 2217.492 kb on + strand at 2217.514 kb on + strand at 2217.515 kb on - strand at 2217.515 kb on - strand at 2217.540 kb on - strand at 2217.540 kb on - strand at 2217.565 kb on - strand at 2217.567 kb on + strand at 2217.568 kb on - strand at 2217.568 kb on - strand at 2217.568 kb on - strand at 2217.659 kb on + strand at 2217.659 kb on + strand at 2217.659 kb on + strand at 2217.659 kb on + strand at 2217.660 kb on - strand at 2217.660 kb on - strand at 2217.660 kb on - strand at 2217.660 kb on - strand at 2217.660 kb on - strand at 2217.660 kb on - strand at 2217.680 kb on + strand at 2217.680 kb on + strand at 2217.680 kb on + strand at 2217.680 kb on + strand at 2217.680 kb on + strand at 2217.680 kb on + strand at 2217.680 kb on + strand at 2217.680 kb on + strand at 2217.680 kb on + strand at 2217.680 kb on + strand at 2217.680 kb on + strand at 2217.680 kb on + strand at 2217.680 kb on + strand at 2217.680 kb on + strand at 2217.680 kb on + strand at 2217.681 kb on - strand at 2217.681 kb on - strand at 2217.681 kb on - strand at 2217.681 kb on - strand at 2217.681 kb on - strand at 2217.681 kb on - strand at 2217.681 kb on - strand at 2217.681 kb on - strand at 2217.681 kb on - strand at 2217.686 kb on + strand at 2217.687 kb on - strand at 2217.688 kb on + strand at 2217.688 kb on + strand at 2217.688 kb on + strand at 2217.689 kb on - strand at 2217.689 kb on - strand at 2217.689 kb on - strand at 2217.689 kb on - strand at 2217.689 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 2,215,759 - tuf NIAGMN_11210 0.15 -0.4 2,216,169 - tuf NIAGMN_11210 0.50 +0.2 2,216,169 - tuf NIAGMN_11210 0.50 -0.7 2,216,777 - +0.3 2,216,788 - -0.8 2,216,844 - +0.2 2,216,844 - +0.5 2,216,918 - Thr_trna NIAGMN_11215 0.53 +0.2 2,217,117 - -1.3 2,217,117 - +0.7 2,217,130 - -0.1 2,217,200 - Tyr_trna NIAGMN_11225 0.58 +1.7 2,217,440 + -0.5 2,217,441 - +0.4 2,217,441 - +1.3 2,217,486 - -0.7 2,217,492 + +0.4 2,217,492 + +0.2 2,217,514 + +0.7 2,217,515 - +0.0 2,217,515 - -0.3 2,217,540 - +0.4 2,217,540 - -0.3 2,217,565 - +0.6 2,217,567 + +2.7 2,217,568 - -0.7 2,217,568 - +0.3 2,217,568 - +1.3 2,217,659 + +0.2 2,217,659 + -0.1 2,217,659 + +0.7 2,217,659 + +1.7 2,217,660 - +0.7 2,217,660 - -0.6 2,217,660 - -0.5 2,217,660 - -1.0 2,217,660 - +2.7 2,217,660 - -0.7 2,217,680 + +0.2 2,217,680 + -0.3 2,217,680 + -0.5 2,217,680 + -0.5 2,217,680 + -0.7 2,217,680 + +0.6 2,217,680 + -0.6 2,217,680 + -0.3 2,217,680 + +0.6 2,217,680 + -1.0 2,217,680 + +0.2 2,217,680 + +0.0 2,217,680 + +1.4 2,217,680 + +0.5 2,217,680 + +0.3 2,217,681 - -0.5 2,217,681 - -1.9 2,217,681 - -0.6 2,217,681 - -0.3 2,217,681 - -1.6 2,217,681 - +0.2 2,217,681 - -1.3 2,217,681 - -0.5 2,217,681 - +0.2 2,217,686 + -0.1 2,217,687 - -0.6 2,217,688 + +1.2 2,217,688 + +0.9 2,217,688 + -0.8 2,217,689 - +1.7 2,217,689 - +0.2 2,217,689 - +0.2 2,217,689 - -0.8 2,217,689 - +0.2
Or see this region's nucleotide sequence