Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepD and prfH are separated by 56 nucleotides prfH and ykfJ overlap by 32 nucleotides ykfJ and yafP are separated by 306 nucleotides yafP and yafO are separated by 84 nucleotides
NIAGMN_07560: pepD - cytosol nonspecific dipeptidase, at 1,448,441 to 1,449,898
pepD
NIAGMN_07565: prfH - peptide chain release factor H, at 1,449,955 to 1,450,455
prfH
NIAGMN_07570: ykfJ - Putative uncharacterized protein YkfJ, at 1,450,424 to 1,450,690
ykfJ
NIAGMN_07575: yafP - GNAT family N-acetyltransferase, at 1,450,997 to 1,451,374
yafP
NIAGMN_07580: yafO - type II toxin-antitoxin system mRNA interferase toxin YafO, at 1,451,459 to 1,451,857
yafO
Position (kb)
1450
1451 Strain fitness (log2 ratio)
-1
0
1
2 at 1449.509 kb on - strand, within pepD at 1449.509 kb on - strand, within pepD at 1449.560 kb on + strand, within pepD at 1449.560 kb on + strand, within pepD at 1449.561 kb on - strand, within pepD at 1449.561 kb on - strand, within pepD at 1449.626 kb on + strand, within pepD at 1449.626 kb on + strand, within pepD at 1449.684 kb on - strand, within pepD at 1449.761 kb on + strand at 1449.785 kb on - strand at 1449.843 kb on + strand at 1449.844 kb on - strand at 1449.844 kb on - strand at 1449.844 kb on - strand at 1449.844 kb on - strand at 1449.900 kb on + strand at 1449.967 kb on - strand at 1450.054 kb on - strand, within prfH at 1450.054 kb on - strand, within prfH at 1450.113 kb on + strand, within prfH at 1450.114 kb on - strand, within prfH at 1450.114 kb on - strand, within prfH at 1450.178 kb on - strand, within prfH at 1450.372 kb on + strand, within prfH at 1450.479 kb on + strand, within ykfJ at 1450.535 kb on + strand, within ykfJ at 1450.536 kb on - strand, within ykfJ at 1450.640 kb on - strand, within ykfJ at 1450.640 kb on - strand, within ykfJ at 1450.643 kb on + strand, within ykfJ at 1450.667 kb on - strand at 1450.669 kb on - strand at 1450.669 kb on - strand at 1450.681 kb on - strand at 1450.704 kb on - strand at 1450.720 kb on - strand at 1450.720 kb on - strand at 1450.752 kb on - strand at 1450.752 kb on - strand at 1450.752 kb on - strand at 1450.756 kb on - strand at 1450.756 kb on - strand at 1450.756 kb on - strand at 1450.756 kb on - strand at 1450.760 kb on - strand at 1450.760 kb on - strand at 1450.815 kb on + strand at 1450.817 kb on + strand at 1450.817 kb on + strand at 1450.819 kb on + strand at 1450.824 kb on - strand at 1451.213 kb on - strand, within yafP at 1451.393 kb on - strand at 1451.399 kb on + strand at 1451.400 kb on - strand at 1451.425 kb on - strand at 1451.439 kb on - strand at 1451.595 kb on + strand, within yafO at 1451.596 kb on - strand, within yafO at 1451.677 kb on + strand, within yafO at 1451.677 kb on + strand, within yafO
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 1,449,509 - pepD NIAGMN_07560 0.73 +1.6 1,449,509 - pepD NIAGMN_07560 0.73 -0.9 1,449,560 + pepD NIAGMN_07560 0.77 +0.1 1,449,560 + pepD NIAGMN_07560 0.77 -0.8 1,449,561 - pepD NIAGMN_07560 0.77 -0.4 1,449,561 - pepD NIAGMN_07560 0.77 +0.6 1,449,626 + pepD NIAGMN_07560 0.81 +1.0 1,449,626 + pepD NIAGMN_07560 0.81 -1.1 1,449,684 - pepD NIAGMN_07560 0.85 +0.8 1,449,761 + -0.4 1,449,785 - +1.6 1,449,843 + +0.6 1,449,844 - +1.6 1,449,844 - +1.6 1,449,844 - -0.0 1,449,844 - -0.2 1,449,900 + +0.4 1,449,967 - -0.0 1,450,054 - prfH NIAGMN_07565 0.20 +0.1 1,450,054 - prfH NIAGMN_07565 0.20 -1.2 1,450,113 + prfH NIAGMN_07565 0.32 -1.1 1,450,114 - prfH NIAGMN_07565 0.32 -0.0 1,450,114 - prfH NIAGMN_07565 0.32 -0.5 1,450,178 - prfH NIAGMN_07565 0.45 +0.5 1,450,372 + prfH NIAGMN_07565 0.83 +0.0 1,450,479 + ykfJ NIAGMN_07570 0.21 -0.2 1,450,535 + ykfJ NIAGMN_07570 0.42 -0.3 1,450,536 - ykfJ NIAGMN_07570 0.42 +0.1 1,450,640 - ykfJ NIAGMN_07570 0.81 -0.8 1,450,640 - ykfJ NIAGMN_07570 0.81 +0.2 1,450,643 + ykfJ NIAGMN_07570 0.82 -0.8 1,450,667 - +1.1 1,450,669 - -0.0 1,450,669 - +1.2 1,450,681 - +0.4 1,450,704 - +1.9 1,450,720 - -0.4 1,450,720 - +0.2 1,450,752 - -0.4 1,450,752 - -0.5 1,450,752 - -0.6 1,450,756 - -0.4 1,450,756 - -0.8 1,450,756 - +0.0 1,450,756 - -0.4 1,450,760 - +0.3 1,450,760 - -0.4 1,450,815 + -1.0 1,450,817 + -0.2 1,450,817 + -0.0 1,450,819 + +1.0 1,450,824 - -0.2 1,451,213 - yafP NIAGMN_07575 0.57 -1.4 1,451,393 - +1.3 1,451,399 + +1.0 1,451,400 - +0.3 1,451,425 - +0.1 1,451,439 - +1.6 1,451,595 + yafO NIAGMN_07580 0.34 +0.6 1,451,596 - yafO NIAGMN_07580 0.34 +0.3 1,451,677 + yafO NIAGMN_07580 0.55 +0.1 1,451,677 + yafO NIAGMN_07580 0.55 +0.2
Or see this region's nucleotide sequence