Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gsiB and gsiA are separated by 19 nucleotides gsiA and iaaA overlap by 14 nucleotides iaaA and gsiA are separated by 12 nucleotides
NIAGMN_04140: gsiB - glutathione ABC transporter substrate-binding protein GsiB, at 745,757 to 747,295
gsiB
NIAGMN_04145: gsiA - glutathione ABC transporter ATP-binding protein GsiA, at 747,315 to 749,186
gsiA
NIAGMN_04150: iaaA - beta-aspartyl-peptidase, at 749,173 to 749,778
iaaA
NIAGMN_04155: gsiA - glutathione ABC transporter ATP-binding protein GsiA, at 749,791 to 750,138
gsiA
Position (kb) 747
748
749
750 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 746.326 kb on - strand, within gsiB at 746.326 kb on - strand, within gsiB at 746.326 kb on - strand, within gsiB at 746.365 kb on + strand, within gsiB at 746.366 kb on - strand, within gsiB at 746.380 kb on - strand, within gsiB at 746.480 kb on - strand, within gsiB at 746.486 kb on - strand, within gsiB at 746.486 kb on - strand, within gsiB at 746.487 kb on + strand, within gsiB at 746.497 kb on + strand, within gsiB at 746.734 kb on - strand, within gsiB at 746.908 kb on + strand, within gsiB at 746.908 kb on + strand, within gsiB at 746.910 kb on + strand, within gsiB at 746.911 kb on - strand, within gsiB at 746.926 kb on + strand, within gsiB at 746.926 kb on + strand, within gsiB at 747.034 kb on - strand, within gsiB at 747.037 kb on + strand, within gsiB at 747.059 kb on - strand, within gsiB at 747.165 kb on + strand at 747.282 kb on - strand at 747.317 kb on - strand at 747.317 kb on - strand at 747.317 kb on - strand at 747.320 kb on + strand at 747.320 kb on + strand at 747.359 kb on - strand at 747.420 kb on - strand at 747.420 kb on - strand at 747.447 kb on + strand at 747.510 kb on - strand, within gsiA at 747.512 kb on - strand, within gsiA at 747.574 kb on + strand, within gsiA at 747.575 kb on - strand, within gsiA at 747.575 kb on - strand, within gsiA at 747.575 kb on - strand, within gsiA at 747.575 kb on - strand, within gsiA at 747.575 kb on - strand, within gsiA at 747.575 kb on - strand, within gsiA at 747.575 kb on - strand, within gsiA at 747.617 kb on + strand, within gsiA at 747.638 kb on - strand, within gsiA at 747.879 kb on - strand, within gsiA at 747.879 kb on - strand, within gsiA at 748.460 kb on - strand, within gsiA at 748.515 kb on - strand, within gsiA at 748.549 kb on - strand, within gsiA at 748.558 kb on - strand, within gsiA at 748.824 kb on - strand, within gsiA at 748.824 kb on - strand, within gsiA at 749.024 kb on + strand at 749.100 kb on + strand at 749.101 kb on - strand at 749.120 kb on + strand at 749.133 kb on - strand at 749.133 kb on - strand at 749.207 kb on + strand at 749.207 kb on + strand at 749.207 kb on + strand at 749.208 kb on - strand at 749.238 kb on - strand, within iaaA at 749.348 kb on - strand, within iaaA at 749.377 kb on + strand, within iaaA at 749.412 kb on - strand, within iaaA at 749.412 kb on - strand, within iaaA at 749.681 kb on - strand, within iaaA at 749.691 kb on - strand, within iaaA at 749.871 kb on + strand, within gsiA at 750.057 kb on - strand, within gsiA at 750.062 kb on - strand, within gsiA at 750.141 kb on + strand at 750.142 kb on - strand at 750.142 kb on - strand at 750.176 kb on - strand at 750.177 kb on + strand at 750.178 kb on - strand at 750.181 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 746,326 - gsiB NIAGMN_04140 0.37 -0.8 746,326 - gsiB NIAGMN_04140 0.37 +1.9 746,326 - gsiB NIAGMN_04140 0.37 -0.9 746,365 + gsiB NIAGMN_04140 0.40 +0.4 746,366 - gsiB NIAGMN_04140 0.40 +0.2 746,380 - gsiB NIAGMN_04140 0.40 +1.4 746,480 - gsiB NIAGMN_04140 0.47 -0.8 746,486 - gsiB NIAGMN_04140 0.47 -0.2 746,486 - gsiB NIAGMN_04140 0.47 +1.4 746,487 + gsiB NIAGMN_04140 0.47 +0.6 746,497 + gsiB NIAGMN_04140 0.48 +1.1 746,734 - gsiB NIAGMN_04140 0.63 +0.2 746,908 + gsiB NIAGMN_04140 0.75 -0.2 746,908 + gsiB NIAGMN_04140 0.75 +0.6 746,910 + gsiB NIAGMN_04140 0.75 -0.8 746,911 - gsiB NIAGMN_04140 0.75 -3.2 746,926 + gsiB NIAGMN_04140 0.76 -0.6 746,926 + gsiB NIAGMN_04140 0.76 -0.2 747,034 - gsiB NIAGMN_04140 0.83 +0.6 747,037 + gsiB NIAGMN_04140 0.83 +0.2 747,059 - gsiB NIAGMN_04140 0.85 +1.5 747,165 + -0.6 747,282 - -0.4 747,317 - +0.5 747,317 - +0.1 747,317 - -0.6 747,320 + +0.4 747,320 + -1.0 747,359 - -0.6 747,420 - -0.5 747,420 - +0.3 747,447 + -1.2 747,510 - gsiA NIAGMN_04145 0.10 +2.9 747,512 - gsiA NIAGMN_04145 0.11 +1.1 747,574 + gsiA NIAGMN_04145 0.14 +0.0 747,575 - gsiA NIAGMN_04145 0.14 -1.8 747,575 - gsiA NIAGMN_04145 0.14 +1.3 747,575 - gsiA NIAGMN_04145 0.14 +0.9 747,575 - gsiA NIAGMN_04145 0.14 -0.4 747,575 - gsiA NIAGMN_04145 0.14 +0.5 747,575 - gsiA NIAGMN_04145 0.14 -0.4 747,575 - gsiA NIAGMN_04145 0.14 +0.5 747,617 + gsiA NIAGMN_04145 0.16 +0.8 747,638 - gsiA NIAGMN_04145 0.17 -0.5 747,879 - gsiA NIAGMN_04145 0.30 -0.4 747,879 - gsiA NIAGMN_04145 0.30 -1.2 748,460 - gsiA NIAGMN_04145 0.61 +1.3 748,515 - gsiA NIAGMN_04145 0.64 +1.3 748,549 - gsiA NIAGMN_04145 0.66 +0.3 748,558 - gsiA NIAGMN_04145 0.66 +1.8 748,824 - gsiA NIAGMN_04145 0.81 +1.8 748,824 - gsiA NIAGMN_04145 0.81 -0.9 749,024 + -0.7 749,100 + +0.8 749,101 - +0.6 749,120 + -1.2 749,133 - +0.3 749,133 - -0.5 749,207 + -0.2 749,207 + -0.4 749,207 + +0.6 749,208 - -2.0 749,238 - iaaA NIAGMN_04150 0.11 -0.4 749,348 - iaaA NIAGMN_04150 0.29 -1.0 749,377 + iaaA NIAGMN_04150 0.34 +1.3 749,412 - iaaA NIAGMN_04150 0.39 -1.2 749,412 - iaaA NIAGMN_04150 0.39 -0.7 749,681 - iaaA NIAGMN_04150 0.84 -0.7 749,691 - iaaA NIAGMN_04150 0.85 -0.1 749,871 + gsiA NIAGMN_04155 0.23 +0.6 750,057 - gsiA NIAGMN_04155 0.76 -0.7 750,062 - gsiA NIAGMN_04155 0.78 +0.1 750,141 + -1.7 750,142 - -2.0 750,142 - +0.1 750,176 - +0.8 750,177 + -0.2 750,178 - -0.3 750,181 + +0.2
Or see this region's nucleotide sequence