Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepN and pncB are separated by 265 nucleotides pncB and asnS are separated by 168 nucleotides
NIAGMN_03085: pepN - aminopeptidase N, at 532,087 to 534,699
pepN
NIAGMN_03090: pncB - nicotinate phosphoribosyltransferase, at 534,965 to 536,167
pncB
NIAGMN_03095: asnS - asparagine--tRNA ligase, at 536,336 to 537,736
asnS
Position (kb) 534
535
536
537 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 534.074 kb on - strand, within pepN at 534.113 kb on - strand, within pepN at 534.504 kb on + strand at 534.505 kb on - strand at 534.721 kb on - strand at 534.721 kb on - strand at 534.723 kb on - strand at 534.723 kb on - strand at 534.742 kb on - strand at 534.742 kb on - strand at 534.755 kb on - strand at 534.755 kb on - strand at 534.790 kb on - strand at 534.822 kb on + strand at 534.823 kb on - strand at 534.823 kb on - strand at 534.823 kb on - strand at 534.831 kb on + strand at 534.857 kb on + strand at 534.858 kb on - strand at 534.858 kb on - strand at 534.858 kb on - strand at 534.896 kb on - strand at 534.897 kb on + strand at 534.897 kb on + strand at 534.898 kb on - strand at 534.930 kb on + strand at 534.964 kb on + strand at 534.964 kb on + strand at 534.964 kb on + strand at 535.016 kb on + strand at 535.017 kb on - strand at 535.017 kb on - strand at 535.018 kb on + strand at 535.018 kb on + strand at 535.018 kb on + strand at 535.018 kb on + strand at 535.018 kb on + strand at 535.018 kb on + strand at 535.019 kb on - strand at 535.019 kb on - strand at 535.019 kb on - strand at 535.052 kb on + strand at 535.055 kb on + strand at 535.055 kb on + strand at 535.056 kb on - strand at 535.056 kb on - strand at 535.108 kb on + strand, within pncB at 535.112 kb on + strand, within pncB at 535.112 kb on + strand, within pncB at 535.175 kb on + strand, within pncB at 535.219 kb on + strand, within pncB at 535.219 kb on + strand, within pncB at 535.219 kb on + strand, within pncB at 535.425 kb on + strand, within pncB at 535.459 kb on + strand, within pncB at 535.459 kb on + strand, within pncB at 535.459 kb on + strand, within pncB at 535.574 kb on + strand, within pncB at 535.662 kb on + strand, within pncB at 535.747 kb on + strand, within pncB at 535.747 kb on + strand, within pncB at 535.747 kb on + strand, within pncB at 535.965 kb on + strand, within pncB at 535.965 kb on + strand, within pncB at 536.023 kb on + strand, within pncB at 536.023 kb on + strand, within pncB at 536.023 kb on + strand, within pncB at 536.023 kb on + strand, within pncB at 536.032 kb on + strand, within pncB at 536.032 kb on + strand, within pncB at 536.032 kb on + strand, within pncB at 536.040 kb on + strand, within pncB at 536.092 kb on + strand at 536.104 kb on + strand at 536.152 kb on + strand at 536.165 kb on + strand at 536.165 kb on + strand at 536.165 kb on + strand at 536.270 kb on + strand at 536.270 kb on + strand at 536.274 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 534,074 - pepN NIAGMN_03085 0.76 +1.3 534,113 - pepN NIAGMN_03085 0.78 +1.3 534,504 + +1.3 534,505 - +2.6 534,721 - +0.5 534,721 - -0.1 534,723 - -0.2 534,723 - +0.6 534,742 - +1.3 534,742 - +1.9 534,755 - -0.3 534,755 - +0.3 534,790 - +2.3 534,822 + +0.7 534,823 - -0.6 534,823 - -0.9 534,823 - +1.7 534,831 + +2.3 534,857 + +2.3 534,858 - -0.3 534,858 - +0.7 534,858 - +0.3 534,896 - +0.4 534,897 + -0.0 534,897 + -0.3 534,898 - +1.3 534,930 + +0.6 534,964 + +2.9 534,964 + -0.4 534,964 + -1.3 535,016 + -0.5 535,017 - -1.2 535,017 - +1.3 535,018 + +0.3 535,018 + +0.3 535,018 + -1.0 535,018 + -1.7 535,018 + -0.0 535,018 + -0.9 535,019 - +1.3 535,019 - +1.3 535,019 - +2.3 535,052 + +0.3 535,055 + +0.1 535,055 + +0.3 535,056 - +0.4 535,056 - +0.0 535,108 + pncB NIAGMN_03090 0.12 -0.2 535,112 + pncB NIAGMN_03090 0.12 -0.3 535,112 + pncB NIAGMN_03090 0.12 +0.3 535,175 + pncB NIAGMN_03090 0.17 +0.3 535,219 + pncB NIAGMN_03090 0.21 +0.3 535,219 + pncB NIAGMN_03090 0.21 +0.1 535,219 + pncB NIAGMN_03090 0.21 -0.7 535,425 + pncB NIAGMN_03090 0.38 -1.5 535,459 + pncB NIAGMN_03090 0.41 -0.0 535,459 + pncB NIAGMN_03090 0.41 +0.7 535,459 + pncB NIAGMN_03090 0.41 -1.0 535,574 + pncB NIAGMN_03090 0.51 -1.4 535,662 + pncB NIAGMN_03090 0.58 -1.9 535,747 + pncB NIAGMN_03090 0.65 +0.9 535,747 + pncB NIAGMN_03090 0.65 -1.5 535,747 + pncB NIAGMN_03090 0.65 -1.0 535,965 + pncB NIAGMN_03090 0.83 -0.3 535,965 + pncB NIAGMN_03090 0.83 -0.6 536,023 + pncB NIAGMN_03090 0.88 -0.1 536,023 + pncB NIAGMN_03090 0.88 -0.8 536,023 + pncB NIAGMN_03090 0.88 +0.6 536,023 + pncB NIAGMN_03090 0.88 -3.1 536,032 + pncB NIAGMN_03090 0.89 +0.3 536,032 + pncB NIAGMN_03090 0.89 -0.3 536,032 + pncB NIAGMN_03090 0.89 -0.6 536,040 + pncB NIAGMN_03090 0.89 -0.9 536,092 + -1.4 536,104 + -0.1 536,152 + -1.7 536,165 + -0.3 536,165 + +1.9 536,165 + -1.9 536,270 + +0.3 536,270 + -0.0 536,274 + -0.0
Or see this region's nucleotide sequence