Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliM and fliN overlap by 4 nucleotides fliN and fliO are separated by 2 nucleotides fliO and fliP overlap by 1 nucleotides fliP and fliQ are separated by 9 nucleotides fliQ and fliR are separated by 7 nucleotides
NIAGMN_02375: fliM - flagellar motor switch protein FliM, at 420,222 to 421,226
fliM
NIAGMN_02380: fliN - flagellar motor switch protein FliN, at 421,223 to 421,636
fliN
NIAGMN_02385: fliO - flagellar type III secretion system protein FliO, at 421,639 to 422,004
fliO
NIAGMN_02390: fliP - flagellar type III secretion system pore protein FliP, at 422,004 to 422,741
fliP
NIAGMN_02395: fliQ - flagellar biosynthesis protein FliQ, at 422,751 to 423,020
fliQ
NIAGMN_02400: fliR - flagellar type III secretion system protein FliR, at 423,028 to 423,813
fliR
Position (kb)
422
423 Strain fitness (log2 ratio)
-1
0
1
2
3 at 421.158 kb on + strand at 421.722 kb on + strand, within fliO at 421.944 kb on - strand, within fliO at 422.259 kb on - strand, within fliP at 422.267 kb on - strand, within fliP at 422.733 kb on + strand at 422.996 kb on + strand at 423.006 kb on + strand at 423.028 kb on - strand at 423.073 kb on + strand at 423.285 kb on + strand, within fliR at 423.388 kb on - strand, within fliR at 423.388 kb on - strand, within fliR at 423.458 kb on + strand, within fliR at 423.459 kb on - strand, within fliR at 423.646 kb on - strand, within fliR
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 421,158 + +0.3 421,722 + fliO NIAGMN_02385 0.23 +0.9 421,944 - fliO NIAGMN_02385 0.83 +1.3 422,259 - fliP NIAGMN_02390 0.35 +0.3 422,267 - fliP NIAGMN_02390 0.36 -1.7 422,733 + -1.0 422,996 + +0.1 423,006 + +0.1 423,028 - +0.7 423,073 + +3.6 423,285 + fliR NIAGMN_02400 0.33 +0.2 423,388 - fliR NIAGMN_02400 0.46 -0.0 423,388 - fliR NIAGMN_02400 0.46 +0.6 423,458 + fliR NIAGMN_02400 0.55 +0.1 423,459 - fliR NIAGMN_02400 0.55 +0.2 423,646 - fliR NIAGMN_02400 0.79 +2.0
Or see this region's nucleotide sequence