Strain Fitness in Escherichia coli ECRC102 around NIAGMN_01835

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyecE and yecN are separated by 52 nucleotidesyecN and cmoA are separated by 40 nucleotidescmoA and cmoB overlap by 4 nucleotides NIAGMN_01825: yecE - UPF0759 protein YecE, at 328,907 to 329,725 yecE NIAGMN_01830: yecN - Inner membrane protein YecN, at 329,778 to 330,173 yecN NIAGMN_01835: cmoA - carboxy-S-adenosyl-L-methionine synthase CmoA, at 330,214 to 330,957 cmoA NIAGMN_01840: cmoB - tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB, at 330,954 to 331,925 cmoB Position (kb) 330 331Strain fitness (log2 ratio) -1 0 1at 329.286 kb on - strand, within yecEat 329.475 kb on + strand, within yecEat 329.605 kb on - strand, within yecEat 329.609 kb on - strand, within yecEat 329.618 kb on - strand, within yecEat 329.644 kb on + strandat 329.645 kb on - strandat 329.661 kb on + strandat 329.662 kb on - strandat 329.666 kb on - strandat 329.736 kb on + strandat 329.736 kb on + strandat 329.737 kb on - strandat 329.737 kb on - strandat 329.737 kb on - strandat 329.934 kb on + strand, within yecNat 329.934 kb on + strand, within yecNat 329.935 kb on - strand, within yecNat 329.936 kb on + strand, within yecNat 330.141 kb on - strandat 330.141 kb on - strandat 330.141 kb on - strandat 330.339 kb on + strand, within cmoAat 330.565 kb on - strand, within cmoAat 331.136 kb on + strand, within cmoBat 331.448 kb on + strand, within cmoBat 331.694 kb on + strand, within cmoBat 331.934 kb on + strandat 331.934 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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329,286 - yecE NIAGMN_01825 0.46 -0.5
329,475 + yecE NIAGMN_01825 0.69 -0.4
329,605 - yecE NIAGMN_01825 0.85 -0.1
329,609 - yecE NIAGMN_01825 0.86 -1.6
329,618 - yecE NIAGMN_01825 0.87 -1.1
329,644 + +0.2
329,645 - +0.6
329,661 + -0.1
329,662 - +0.4
329,666 - +0.3
329,736 + +0.2
329,736 + +0.5
329,737 - +1.2
329,737 - +0.2
329,737 - +0.2
329,934 + yecN NIAGMN_01830 0.39 -0.4
329,934 + yecN NIAGMN_01830 0.39 -0.8
329,935 - yecN NIAGMN_01830 0.40 -0.2
329,936 + yecN NIAGMN_01830 0.40 +1.2
330,141 - +0.7
330,141 - -0.7
330,141 - +0.2
330,339 + cmoA NIAGMN_01835 0.17 -1.4
330,565 - cmoA NIAGMN_01835 0.47 +1.6
331,136 + cmoB NIAGMN_01840 0.19 -0.4
331,448 + cmoB NIAGMN_01840 0.51 +0.6
331,694 + cmoB NIAGMN_01840 0.76 -0.2
331,934 + -0.6
331,934 + -0.6

Or see this region's nucleotide sequence