Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lhr and grxD are separated by 50 nucleotides grxD and mepH are separated by 334 nucleotides mepH and sodB are separated by 127 nucleotides sodB and ydhP are separated by 145 nucleotides
NIAGMN_00645: lhr - ATP-dependent helicase, at 104,384 to 109,000
lhr
NIAGMN_00650: grxD - monothiol glutaredoxin 4, at 109,051 to 109,398
grxD
NIAGMN_00655: mepH - peptidoglycan DD-endopeptidase MepH, at 109,733 to 110,548
mepH
NIAGMN_00660: sodB - superoxide dismutase [Fe], at 110,676 to 111,257
sodB
NIAGMN_00665: ydhP - Inner membrane transport protein YdhP, at 111,403 to 112,572
ydhP
Position (kb) 109
110
111 Strain fitness (log2 ratio) -1
0
1
2 at 108.943 kb on + strand at 109.080 kb on - strand at 109.080 kb on - strand at 109.128 kb on - strand, within grxD at 109.201 kb on - strand, within grxD at 109.201 kb on - strand, within grxD at 109.201 kb on - strand, within grxD at 109.201 kb on - strand, within grxD at 109.206 kb on - strand, within grxD at 109.253 kb on + strand, within grxD at 109.423 kb on - strand at 109.492 kb on + strand at 109.498 kb on - strand at 109.668 kb on - strand at 110.170 kb on + strand, within mepH at 110.341 kb on + strand, within mepH at 110.490 kb on - strand at 110.637 kb on + strand at 110.646 kb on + strand at 110.698 kb on + strand at 110.752 kb on - strand, within sodB at 110.787 kb on - strand, within sodB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 108,943 + -1.1 109,080 - +0.2 109,080 - -0.1 109,128 - grxD NIAGMN_00650 0.22 +0.2 109,201 - grxD NIAGMN_00650 0.43 -0.2 109,201 - grxD NIAGMN_00650 0.43 -1.3 109,201 - grxD NIAGMN_00650 0.43 -1.5 109,201 - grxD NIAGMN_00650 0.43 +1.0 109,206 - grxD NIAGMN_00650 0.45 -0.3 109,253 + grxD NIAGMN_00650 0.58 +2.2 109,423 - -1.1 109,492 + +1.2 109,498 - +2.2 109,668 - -1.4 110,170 + mepH NIAGMN_00655 0.54 -0.4 110,341 + mepH NIAGMN_00655 0.75 -1.6 110,490 - +1.2 110,637 + -1.0 110,646 + -0.4 110,698 + +0.5 110,752 - sodB NIAGMN_00660 0.13 -0.4 110,787 - sodB NIAGMN_00660 0.19 +0.6
Or see this region's nucleotide sequence