Experiment: R2A_PIPES pH 4.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tig and Leu_trna are separated by 92 nucleotides Leu_trna and Lys_trna are separated by 274 nucleotides Lys_trna and His_trna are separated by 20 nucleotides His_trna and Arg_trna are separated by 13 nucleotides Arg_trna and Pro_trna are separated by 24 nucleotides Pro_trna and Arg_trna are separated by 177 nucleotides
OKGIIK_08045: tig - trigger factor, at 1,776,995 to 1,778,287
tig
OKGIIK_08050: Leu_trna - tRNA-Leu, at 1,778,380 to 1,778,464
_trna
OKGIIK_08055: Lys_trna - tRNA-Lys, at 1,778,739 to 1,778,814
_trna
OKGIIK_08060: His_trna - tRNA-His, at 1,778,835 to 1,778,911
_trna
OKGIIK_08065: Arg_trna - tRNA-Arg, at 1,778,925 to 1,779,001
_trna
OKGIIK_08070: Pro_trna - tRNA-Pro, at 1,779,026 to 1,779,102
_trna
OKGIIK_08075: Arg_trna - tRNA-Arg, at 1,779,280 to 1,779,356
_trna
Position (kb)
1778
1779 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1777.785 kb on + strand, within tig at 1777.786 kb on - strand, within tig at 1777.786 kb on - strand, within tig at 1777.974 kb on + strand, within tig at 1777.974 kb on + strand, within tig at 1777.974 kb on + strand, within tig at 1778.300 kb on + strand at 1778.300 kb on + strand at 1778.300 kb on + strand at 1778.300 kb on + strand at 1778.301 kb on - strand at 1778.301 kb on - strand at 1778.301 kb on - strand at 1778.303 kb on + strand at 1778.303 kb on + strand at 1778.303 kb on + strand at 1778.303 kb on + strand at 1778.303 kb on + strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.361 kb on + strand at 1778.361 kb on + strand at 1778.362 kb on - strand at 1778.362 kb on - strand at 1778.486 kb on - strand at 1778.486 kb on - strand at 1778.486 kb on - strand at 1778.486 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.494 kb on - strand at 1778.494 kb on - strand at 1778.494 kb on - strand at 1778.494 kb on - strand at 1778.494 kb on - strand at 1778.568 kb on - strand at 1778.568 kb on - strand at 1778.568 kb on - strand at 1778.568 kb on - strand at 1778.568 kb on - strand at 1778.568 kb on - strand at 1778.568 kb on - strand at 1778.568 kb on - strand at 1778.568 kb on - strand at 1778.584 kb on + strand at 1778.585 kb on - strand at 1778.587 kb on - strand at 1778.587 kb on - strand at 1778.587 kb on - strand at 1778.587 kb on - strand at 1778.605 kb on - strand at 1778.605 kb on - strand at 1778.605 kb on - strand at 1778.605 kb on - strand at 1778.605 kb on - strand at 1778.923 kb on - strand at 1779.275 kb on - strand at 1779.275 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.5 remove 1,777,785 + tig OKGIIK_08045 0.61 -0.3 1,777,786 - tig OKGIIK_08045 0.61 -0.3 1,777,786 - tig OKGIIK_08045 0.61 +0.6 1,777,974 + tig OKGIIK_08045 0.76 +1.2 1,777,974 + tig OKGIIK_08045 0.76 +0.1 1,777,974 + tig OKGIIK_08045 0.76 +1.3 1,778,300 + +0.2 1,778,300 + -0.6 1,778,300 + +0.7 1,778,300 + -0.2 1,778,301 - +1.8 1,778,301 - +1.5 1,778,301 - -0.8 1,778,303 + +0.7 1,778,303 + -0.0 1,778,303 + +0.1 1,778,303 + -1.1 1,778,303 + -1.8 1,778,304 - +0.2 1,778,304 - +0.7 1,778,304 - -1.5 1,778,304 - +1.1 1,778,304 - +0.2 1,778,304 - +0.7 1,778,304 - -0.5 1,778,304 - +1.5 1,778,304 - +1.7 1,778,304 - +1.5 1,778,304 - -0.6 1,778,361 + -0.6 1,778,361 + +1.9 1,778,362 - -0.3 1,778,362 - -0.3 1,778,486 - -1.2 1,778,486 - +1.4 1,778,486 - -0.4 1,778,486 - -0.7 1,778,490 - -1.6 1,778,490 - -0.6 1,778,490 - -0.9 1,778,490 - +0.4 1,778,490 - -0.3 1,778,490 - -0.1 1,778,490 - -1.0 1,778,490 - -1.5 1,778,490 - +1.7 1,778,490 - -0.3 1,778,490 - +1.1 1,778,490 - -1.1 1,778,490 - -1.5 1,778,490 - -1.3 1,778,490 - -1.6 1,778,494 - -1.1 1,778,494 - -0.3 1,778,494 - -0.4 1,778,494 - -0.1 1,778,494 - -0.6 1,778,568 - -0.9 1,778,568 - -0.2 1,778,568 - -0.3 1,778,568 - -0.6 1,778,568 - -1.8 1,778,568 - +0.0 1,778,568 - -0.9 1,778,568 - -0.6 1,778,568 - -0.4 1,778,584 + -0.3 1,778,585 - +1.6 1,778,587 - -1.9 1,778,587 - -0.3 1,778,587 - -0.2 1,778,587 - +0.9 1,778,605 - -1.8 1,778,605 - -0.6 1,778,605 - -1.1 1,778,605 - -0.8 1,778,605 - -0.6 1,778,923 - -1.0 1,779,275 - +0.7 1,779,275 - -0.5
Or see this region's nucleotide sequence