Experiment: R2A_PIPES pH 4.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaF and OKGIIK_05625 are separated by 35 nucleotides OKGIIK_05625 and elaC overlap by 4 nucleotides elaC and Ser_trna are separated by 92 nucleotides
OKGIIK_05620: rfaF - ADP-heptose--LPS heptosyltransferase, at 1,239,294 to 1,240,352
rfaF
OKGIIK_05625: OKGIIK_05625 - 3-deoxy-D-manno-octulosonic acid kinase, at 1,240,388 to 1,241,119
_05625
OKGIIK_05630: elaC - Ribonuclease BN, tRNA processing enzyme, at 1,241,116 to 1,241,874
elaC
OKGIIK_05635: Ser_trna - tRNA-Ser, at 1,241,967 to 1,242,056
_trna
Position (kb)
1240
1241
1242 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1239.445 kb on + strand, within rfaF at 1239.445 kb on + strand, within rfaF at 1239.445 kb on + strand, within rfaF at 1239.445 kb on + strand, within rfaF at 1239.445 kb on + strand, within rfaF at 1239.445 kb on + strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.475 kb on + strand, within rfaF at 1239.475 kb on + strand, within rfaF at 1239.476 kb on - strand, within rfaF at 1239.476 kb on - strand, within rfaF at 1239.757 kb on + strand, within rfaF at 1239.757 kb on + strand, within rfaF at 1239.758 kb on - strand, within rfaF at 1240.365 kb on - strand at 1240.365 kb on - strand at 1240.365 kb on - strand at 1240.365 kb on - strand at 1240.487 kb on + strand, within OKGIIK_05625 at 1240.487 kb on + strand, within OKGIIK_05625 at 1240.488 kb on - strand, within OKGIIK_05625 at 1240.488 kb on - strand, within OKGIIK_05625 at 1240.583 kb on + strand, within OKGIIK_05625 at 1240.583 kb on + strand, within OKGIIK_05625 at 1240.583 kb on + strand, within OKGIIK_05625 at 1240.584 kb on - strand, within OKGIIK_05625 at 1240.584 kb on - strand, within OKGIIK_05625 at 1240.625 kb on + strand, within OKGIIK_05625 at 1240.730 kb on + strand, within OKGIIK_05625 at 1240.730 kb on + strand, within OKGIIK_05625 at 1240.730 kb on + strand, within OKGIIK_05625 at 1240.730 kb on + strand, within OKGIIK_05625 at 1240.730 kb on + strand, within OKGIIK_05625 at 1240.731 kb on - strand, within OKGIIK_05625 at 1240.751 kb on + strand, within OKGIIK_05625 at 1240.752 kb on - strand, within OKGIIK_05625 at 1240.752 kb on - strand, within OKGIIK_05625 at 1240.752 kb on - strand, within OKGIIK_05625 at 1240.752 kb on - strand, within OKGIIK_05625 at 1240.861 kb on - strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.938 kb on - strand, within OKGIIK_05625 at 1240.938 kb on - strand, within OKGIIK_05625 at 1241.090 kb on + strand at 1241.090 kb on + strand at 1241.090 kb on + strand at 1241.090 kb on + strand at 1241.090 kb on + strand at 1241.090 kb on + strand at 1241.090 kb on + strand at 1241.090 kb on + strand at 1241.091 kb on - strand at 1241.091 kb on - strand at 1241.091 kb on - strand at 1241.097 kb on - strand at 1241.104 kb on + strand at 1241.104 kb on + strand at 1241.104 kb on + strand at 1241.248 kb on + strand, within elaC at 1241.249 kb on - strand, within elaC at 1241.249 kb on - strand, within elaC at 1241.261 kb on - strand, within elaC at 1241.564 kb on - strand, within elaC at 1241.594 kb on - strand, within elaC at 1241.709 kb on + strand, within elaC at 1241.722 kb on + strand, within elaC at 1241.755 kb on + strand, within elaC at 1241.756 kb on - strand, within elaC at 1241.756 kb on - strand, within elaC at 1241.761 kb on + strand, within elaC at 1241.762 kb on - strand, within elaC
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.5 remove 1,239,445 + rfaF OKGIIK_05620 0.14 +0.7 1,239,445 + rfaF OKGIIK_05620 0.14 -0.9 1,239,445 + rfaF OKGIIK_05620 0.14 -0.2 1,239,445 + rfaF OKGIIK_05620 0.14 -1.1 1,239,445 + rfaF OKGIIK_05620 0.14 -0.8 1,239,445 + rfaF OKGIIK_05620 0.14 +1.7 1,239,446 - rfaF OKGIIK_05620 0.14 +0.9 1,239,446 - rfaF OKGIIK_05620 0.14 +0.7 1,239,446 - rfaF OKGIIK_05620 0.14 +1.6 1,239,446 - rfaF OKGIIK_05620 0.14 +1.3 1,239,446 - rfaF OKGIIK_05620 0.14 +1.9 1,239,446 - rfaF OKGIIK_05620 0.14 +0.8 1,239,446 - rfaF OKGIIK_05620 0.14 +1.0 1,239,475 + rfaF OKGIIK_05620 0.17 +1.4 1,239,475 + rfaF OKGIIK_05620 0.17 +1.7 1,239,476 - rfaF OKGIIK_05620 0.17 +1.9 1,239,476 - rfaF OKGIIK_05620 0.17 -1.8 1,239,757 + rfaF OKGIIK_05620 0.44 +2.1 1,239,757 + rfaF OKGIIK_05620 0.44 +0.4 1,239,758 - rfaF OKGIIK_05620 0.44 +0.7 1,240,365 - -0.5 1,240,365 - +1.1 1,240,365 - +0.7 1,240,365 - +1.0 1,240,487 + OKGIIK_05625 0.14 +2.6 1,240,487 + OKGIIK_05625 0.14 +1.2 1,240,488 - OKGIIK_05625 0.14 +2.7 1,240,488 - OKGIIK_05625 0.14 +1.9 1,240,583 + OKGIIK_05625 0.27 +2.9 1,240,583 + OKGIIK_05625 0.27 +0.5 1,240,583 + OKGIIK_05625 0.27 +2.0 1,240,584 - OKGIIK_05625 0.27 +2.3 1,240,584 - OKGIIK_05625 0.27 +1.7 1,240,625 + OKGIIK_05625 0.32 +0.5 1,240,730 + OKGIIK_05625 0.47 +0.7 1,240,730 + OKGIIK_05625 0.47 +2.1 1,240,730 + OKGIIK_05625 0.47 +2.9 1,240,730 + OKGIIK_05625 0.47 +1.8 1,240,730 + OKGIIK_05625 0.47 +3.1 1,240,731 - OKGIIK_05625 0.47 +1.7 1,240,751 + OKGIIK_05625 0.50 +1.1 1,240,752 - OKGIIK_05625 0.50 +1.4 1,240,752 - OKGIIK_05625 0.50 +0.9 1,240,752 - OKGIIK_05625 0.50 +1.8 1,240,752 - OKGIIK_05625 0.50 +1.8 1,240,861 - OKGIIK_05625 0.65 +1.0 1,240,937 + OKGIIK_05625 0.75 +2.2 1,240,937 + OKGIIK_05625 0.75 +2.7 1,240,937 + OKGIIK_05625 0.75 +0.4 1,240,937 + OKGIIK_05625 0.75 +0.4 1,240,937 + OKGIIK_05625 0.75 +2.3 1,240,937 + OKGIIK_05625 0.75 +0.9 1,240,937 + OKGIIK_05625 0.75 +3.0 1,240,937 + OKGIIK_05625 0.75 +2.1 1,240,937 + OKGIIK_05625 0.75 +1.5 1,240,938 - OKGIIK_05625 0.75 +0.3 1,240,938 - OKGIIK_05625 0.75 +2.5 1,241,090 + +3.7 1,241,090 + +1.1 1,241,090 + +3.0 1,241,090 + -0.3 1,241,090 + +2.0 1,241,090 + +1.5 1,241,090 + +2.8 1,241,090 + +2.5 1,241,091 - +1.1 1,241,091 - +0.9 1,241,091 - +3.2 1,241,097 - +2.7 1,241,104 + +0.9 1,241,104 + +0.9 1,241,104 + -1.3 1,241,248 + elaC OKGIIK_05630 0.17 -0.5 1,241,249 - elaC OKGIIK_05630 0.18 +0.9 1,241,249 - elaC OKGIIK_05630 0.18 +0.7 1,241,261 - elaC OKGIIK_05630 0.19 +0.4 1,241,564 - elaC OKGIIK_05630 0.59 -0.3 1,241,594 - elaC OKGIIK_05630 0.63 +0.2 1,241,709 + elaC OKGIIK_05630 0.78 -0.9 1,241,722 + elaC OKGIIK_05630 0.80 -0.3 1,241,755 + elaC OKGIIK_05630 0.84 -1.9 1,241,756 - elaC OKGIIK_05630 0.84 -2.2 1,241,756 - elaC OKGIIK_05630 0.84 +0.2 1,241,761 + elaC OKGIIK_05630 0.85 -0.9 1,241,762 - elaC OKGIIK_05630 0.85 -0.3
Or see this region's nucleotide sequence