Strain Fitness in Escherichia coli ECRC102 around NIAGMN_27675

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpurU and Tyr_trna are separated by 159 nucleotidesTyr_trna and Tyr_trna are separated by 209 nucleotidesTyr_trna and narI are separated by 539 nucleotides NIAGMN_27665: purU - formyltetrahydrofolate deformylase, at 5,360,121 to 5,360,963 purU NIAGMN_27670: Tyr_trna - tRNA-Tyr(gta), at 5,361,123 to 5,361,207 _trna NIAGMN_27675: Tyr_trna - tRNA-Tyr(gta), at 5,361,417 to 5,361,501 _trna NIAGMN_27680: narI - respiratory nitrate reductase subunit gamma, at 5,362,041 to 5,362,718 narI Position (kb) 5361 5362Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5360.439 kb on + strand, within purUat 5360.439 kb on + strand, within purUat 5360.486 kb on + strand, within purUat 5360.491 kb on + strand, within purUat 5360.518 kb on + strand, within purUat 5360.549 kb on - strand, within purUat 5360.625 kb on + strand, within purUat 5360.757 kb on - strand, within purUat 5360.775 kb on + strand, within purUat 5360.893 kb on + strandat 5361.363 kb on + strandat 5361.585 kb on - strandat 5361.930 kb on - strandat 5361.930 kb on - strandat 5362.070 kb on + strandat 5362.071 kb on - strandat 5362.269 kb on - strand, within narI

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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5,360,439 + purU NIAGMN_27665 0.38 +0.1
5,360,439 + purU NIAGMN_27665 0.38 -1.5
5,360,486 + purU NIAGMN_27665 0.43 -1.4
5,360,491 + purU NIAGMN_27665 0.44 -1.6
5,360,518 + purU NIAGMN_27665 0.47 +0.5
5,360,549 - purU NIAGMN_27665 0.51 -2.0
5,360,625 + purU NIAGMN_27665 0.60 +3.2
5,360,757 - purU NIAGMN_27665 0.75 -0.6
5,360,775 + purU NIAGMN_27665 0.78 -0.4
5,360,893 + -1.7
5,361,363 + -0.1
5,361,585 - -0.1
5,361,930 - +0.7
5,361,930 - -1.3
5,362,070 + +0.3
5,362,071 - +2.6
5,362,269 - narI NIAGMN_27680 0.34 -0.1

Or see this region's nucleotide sequence