Strain Fitness in Escherichia coli ECRC102 around NIAGMN_26425

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntuspE and ynaJ are separated by 49 nucleotidesynaJ and ynaI are separated by 243 nucleotidesynaI and mppA are separated by 50 nucleotides NIAGMN_26415: uspE - universal stress protein UspE, at 5,139,730 to 5,140,680 uspE NIAGMN_26420: ynaJ - Uncharacterized protein YnaJ, at 5,140,730 to 5,140,987 ynaJ NIAGMN_26425: ynaI - low conductance mechanosensitive channel YnaI, at 5,141,231 to 5,142,262 ynaI NIAGMN_26430: mppA - murein tripeptide ABC transporter substrate-binding protein MppA, at 5,142,313 to 5,143,926 mppA Position (kb) 5141 5142 5143Strain fitness (log2 ratio) -1 0 1at 5140.731 kb on + strandat 5140.801 kb on - strand, within ynaJat 5140.831 kb on - strand, within ynaJat 5140.831 kb on - strand, within ynaJat 5140.875 kb on - strand, within ynaJat 5140.875 kb on - strand, within ynaJat 5140.993 kb on - strandat 5140.993 kb on - strandat 5141.089 kb on + strandat 5141.477 kb on - strand, within ynaIat 5142.224 kb on + strandat 5142.232 kb on + strandat 5142.330 kb on - strandat 5142.412 kb on - strandat 5142.976 kb on + strand, within mppAat 5143.049 kb on + strand, within mppAat 5143.050 kb on - strand, within mppAat 5143.164 kb on + strand, within mppA

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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5,140,731 + -0.4
5,140,801 - ynaJ NIAGMN_26420 0.28 -0.4
5,140,831 - ynaJ NIAGMN_26420 0.39 +0.0
5,140,831 - ynaJ NIAGMN_26420 0.39 +1.6
5,140,875 - ynaJ NIAGMN_26420 0.56 +1.4
5,140,875 - ynaJ NIAGMN_26420 0.56 -1.0
5,140,993 - +0.4
5,140,993 - -0.7
5,141,089 + +0.0
5,141,477 - ynaI NIAGMN_26425 0.24 -0.4
5,142,224 + -1.7
5,142,232 + -0.7
5,142,330 - +0.7
5,142,412 - -0.4
5,142,976 + mppA NIAGMN_26430 0.41 +0.3
5,143,049 + mppA NIAGMN_26430 0.46 -0.9
5,143,050 - mppA NIAGMN_26430 0.46 +1.4
5,143,164 + mppA NIAGMN_26430 0.53 +0.0

Or see this region's nucleotide sequence