Experiment: EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hinT and NIAGMN_24310 are separated by 2 nucleotides NIAGMN_24310 and lpoB are separated by 13 nucleotides lpoB and thiK overlap by 20 nucleotides thiK and nagZ are separated by 10 nucleotides
NIAGMN_24305: hinT - purine nucleoside phosphoramidase, at 4,754,740 to 4,755,099
hinT
NIAGMN_24310: NIAGMN_24310 - DUF1425 domain-containing protein, at 4,755,102 to 4,755,479
_24310
NIAGMN_24315: lpoB - penicillin-binding protein activator LpoB, at 4,755,493 to 4,756,134
lpoB
NIAGMN_24320: thiK - thiamine kinase, at 4,756,115 to 4,756,939
thiK
NIAGMN_24325: nagZ - beta-N-acetylhexosaminidase, at 4,756,950 to 4,757,975
nagZ
Position (kb) 4755
4756
4757 Strain fitness (log2 ratio) -1
0
1
2 at 4754.596 kb on - strand at 4754.600 kb on - strand at 4754.640 kb on - strand at 4754.670 kb on + strand at 4754.675 kb on + strand at 4755.760 kb on - strand, within lpoB at 4756.199 kb on - strand, within thiK at 4756.318 kb on - strand, within thiK at 4756.356 kb on + strand, within thiK at 4756.356 kb on + strand, within thiK at 4756.356 kb on + strand, within thiK at 4756.385 kb on + strand, within thiK at 4756.508 kb on + strand, within thiK at 4756.797 kb on + strand, within thiK at 4756.929 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EV240 remove 4,754,596 - -0.5 4,754,600 - -0.3 4,754,640 - -0.6 4,754,670 + -0.1 4,754,675 + +2.1 4,755,760 - lpoB NIAGMN_24315 0.42 -0.2 4,756,199 - thiK NIAGMN_24320 0.10 +2.5 4,756,318 - thiK NIAGMN_24320 0.25 +0.2 4,756,356 + thiK NIAGMN_24320 0.29 +1.5 4,756,356 + thiK NIAGMN_24320 0.29 +0.0 4,756,356 + thiK NIAGMN_24320 0.29 +0.2 4,756,385 + thiK NIAGMN_24320 0.33 +0.3 4,756,508 + thiK NIAGMN_24320 0.48 -1.2 4,756,797 + thiK NIAGMN_24320 0.83 +1.1 4,756,929 + +0.5
Or see this region's nucleotide sequence