Experiment: EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_23420 and Ile2_trna are separated by 441 nucleotides Ile2_trna and Arg_trna are separated by 9 nucleotides Arg_trna and Arg_trna are separated by 13 nucleotides Arg_trna and stxA2c are separated by 90 nucleotides stxA2c and stxB2a are separated by 11 nucleotides stxB2a and NIAGMN_23460 are separated by 486 nucleotides
NIAGMN_23420: NIAGMN_23420 - Antitermination protein Q, at 4,587,052 to 4,587,486
_23420
NIAGMN_23425: Ile2_trna - tRNA-Ile2(cat), at 4,587,928 to 4,588,003
_trna
NIAGMN_23430: Arg_trna - tRNA-Arg(tcg), at 4,588,013 to 4,588,089
_trna
NIAGMN_23435: Arg_trna - tRNA-Arg(tct), at 4,588,103 to 4,588,179
_trna
NIAGMN_23440: stxA2c - Shiga toxin Stx2c subunit A, at 4,588,270 to 4,589,229
stxA2c
NIAGMN_23445: stxB2a - Shiga toxin Stx2a subunit B, at 4,589,241 to 4,589,510
stxB2a
NIAGMN_23460: NIAGMN_23460 - sialate O-acetylesterase, at 4,589,997 to 4,591,934
_23460
Position (kb)
4588
4589
4590 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4587.271 kb on + strand, within NIAGMN_23420 at 4587.274 kb on + strand, within NIAGMN_23420 at 4587.643 kb on + strand at 4587.643 kb on + strand at 4587.643 kb on + strand at 4587.664 kb on + strand at 4587.669 kb on + strand at 4587.869 kb on - strand at 4588.004 kb on + strand at 4588.004 kb on + strand at 4588.004 kb on + strand at 4588.092 kb on - strand at 4588.101 kb on - strand at 4588.101 kb on - strand at 4588.180 kb on + strand at 4588.372 kb on + strand, within stxA2c at 4588.395 kb on - strand, within stxA2c at 4588.427 kb on + strand, within stxA2c at 4588.626 kb on + strand, within stxA2c at 4588.626 kb on + strand, within stxA2c at 4588.627 kb on - strand, within stxA2c at 4588.628 kb on + strand, within stxA2c at 4588.629 kb on - strand, within stxA2c at 4588.779 kb on + strand, within stxA2c at 4588.856 kb on + strand, within stxA2c at 4589.154 kb on + strand at 4589.154 kb on + strand at 4589.210 kb on + strand at 4589.215 kb on - strand at 4589.336 kb on + strand, within stxB2a at 4589.498 kb on + strand at 4589.501 kb on + strand at 4589.501 kb on + strand at 4589.531 kb on - strand at 4589.548 kb on - strand at 4589.735 kb on + strand at 4589.752 kb on + strand at 4589.752 kb on + strand at 4589.754 kb on + strand at 4589.754 kb on + strand at 4589.754 kb on + strand at 4589.755 kb on - strand at 4589.755 kb on - strand at 4589.785 kb on - strand at 4589.791 kb on - strand at 4589.791 kb on - strand at 4589.827 kb on + strand at 4589.828 kb on - strand at 4589.837 kb on - strand at 4589.867 kb on + strand at 4589.910 kb on - strand at 4589.975 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EV240 remove 4,587,271 + NIAGMN_23420 0.50 -1.7 4,587,274 + NIAGMN_23420 0.51 +0.2 4,587,643 + +0.5 4,587,643 + +1.6 4,587,643 + -0.0 4,587,664 + -0.1 4,587,669 + -2.2 4,587,869 - +1.0 4,588,004 + +2.9 4,588,004 + -2.2 4,588,004 + -0.8 4,588,092 - +0.2 4,588,101 - +0.6 4,588,101 - -0.7 4,588,180 + +1.9 4,588,372 + stxA2c NIAGMN_23440 0.11 +0.4 4,588,395 - stxA2c NIAGMN_23440 0.13 +0.4 4,588,427 + stxA2c NIAGMN_23440 0.16 -0.8 4,588,626 + stxA2c NIAGMN_23440 0.37 +1.6 4,588,626 + stxA2c NIAGMN_23440 0.37 +0.8 4,588,627 - stxA2c NIAGMN_23440 0.37 -1.0 4,588,628 + stxA2c NIAGMN_23440 0.37 -1.2 4,588,629 - stxA2c NIAGMN_23440 0.37 +0.9 4,588,779 + stxA2c NIAGMN_23440 0.53 -1.6 4,588,856 + stxA2c NIAGMN_23440 0.61 -0.6 4,589,154 + -0.2 4,589,154 + -0.4 4,589,210 + +1.2 4,589,215 - +2.0 4,589,336 + stxB2a NIAGMN_23445 0.35 -0.1 4,589,498 + -2.5 4,589,501 + -1.2 4,589,501 + -1.1 4,589,531 - -0.7 4,589,548 - +0.5 4,589,735 + +0.6 4,589,752 + +0.9 4,589,752 + -0.4 4,589,754 + -3.2 4,589,754 + +0.4 4,589,754 + -0.7 4,589,755 - +0.1 4,589,755 - -1.0 4,589,785 - -0.7 4,589,791 - -1.0 4,589,791 - -2.7 4,589,827 + +1.6 4,589,828 - +0.0 4,589,837 - +1.3 4,589,867 + -0.3 4,589,910 - +0.0 4,589,975 - +0.0
Or see this region's nucleotide sequence