Strain Fitness in Escherichia coli ECRC102 around NIAGMN_22280

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaD and wcaE are separated by 10 nucleotideswcaE and wcaF are separated by 15 nucleotideswcaF and NIAGMN_22290 are separated by 28 nucleotides NIAGMN_22275: wcaD - putative colanic acid polymerase WcaD, at 4,394,119 to 4,395,336 wcaD NIAGMN_22280: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 4,395,347 to 4,396,093 wcaE NIAGMN_22285: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 4,396,109 to 4,396,657 wcaF NIAGMN_22290: NIAGMN_22290 - GDP-mannose 4,6-dehydratase, at 4,396,686 to 4,397,807 _22290 Position (kb) 4395 4396 4397Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4394.355 kb on + strand, within wcaDat 4394.356 kb on - strand, within wcaDat 4394.356 kb on - strand, within wcaDat 4394.356 kb on - strand, within wcaDat 4394.360 kb on - strand, within wcaDat 4394.374 kb on - strand, within wcaDat 4394.463 kb on - strand, within wcaDat 4394.579 kb on - strand, within wcaDat 4395.515 kb on - strand, within wcaEat 4395.515 kb on - strand, within wcaEat 4396.002 kb on + strand, within wcaEat 4396.306 kb on + strand, within wcaFat 4396.329 kb on + strand, within wcaFat 4396.369 kb on + strand, within wcaFat 4396.383 kb on + strand, within wcaFat 4396.421 kb on + strand, within wcaFat 4396.655 kb on + strandat 4396.680 kb on + strandat 4396.681 kb on - strandat 4396.886 kb on + strand, within NIAGMN_22290at 4396.936 kb on - strand, within NIAGMN_22290at 4396.988 kb on + strand, within NIAGMN_22290at 4396.989 kb on - strand, within NIAGMN_22290

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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4,394,355 + wcaD NIAGMN_22275 0.19 +2.1
4,394,356 - wcaD NIAGMN_22275 0.19 +2.7
4,394,356 - wcaD NIAGMN_22275 0.19 +1.7
4,394,356 - wcaD NIAGMN_22275 0.19 -0.9
4,394,360 - wcaD NIAGMN_22275 0.20 -0.2
4,394,374 - wcaD NIAGMN_22275 0.21 -0.2
4,394,463 - wcaD NIAGMN_22275 0.28 +0.7
4,394,579 - wcaD NIAGMN_22275 0.38 +0.7
4,395,515 - wcaE NIAGMN_22280 0.22 -0.7
4,395,515 - wcaE NIAGMN_22280 0.22 -0.7
4,396,002 + wcaE NIAGMN_22280 0.88 -0.1
4,396,306 + wcaF NIAGMN_22285 0.36 -0.3
4,396,329 + wcaF NIAGMN_22285 0.40 -1.1
4,396,369 + wcaF NIAGMN_22285 0.47 -0.7
4,396,383 + wcaF NIAGMN_22285 0.50 -1.8
4,396,421 + wcaF NIAGMN_22285 0.57 -0.3
4,396,655 + -3.0
4,396,680 + -0.3
4,396,681 - -0.6
4,396,886 + NIAGMN_22290 0.18 +0.7
4,396,936 - NIAGMN_22290 0.22 +0.7
4,396,988 + NIAGMN_22290 0.27 -0.7
4,396,989 - NIAGMN_22290 0.27 +0.7

Or see this region's nucleotide sequence