Strain Fitness in Escherichia coli ECRC102 around NIAGMN_20720

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmenH and menB are separated by 14 nucleotidesmenB and menC overlap by 1 nucleotidesmenC and menE overlap by 4 nucleotides NIAGMN_20710: menH - 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxy late synthase, at 4,093,566 to 4,094,324 menH NIAGMN_20715: menB - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 4,094,339 to 4,095,196 menB NIAGMN_20720: menC - o-succinylbenzoate synthase, at 4,095,196 to 4,096,158 menC NIAGMN_20725: menE - o-succinylbenzoate--CoA ligase, at 4,096,155 to 4,097,510 menE Position (kb) 4095 4096 4097Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4094.261 kb on + strandat 4094.262 kb on - strandat 4094.327 kb on - strandat 4094.338 kb on + strandat 4094.646 kb on + strand, within menBat 4094.647 kb on - strand, within menBat 4094.687 kb on - strand, within menBat 4094.782 kb on + strand, within menBat 4094.846 kb on + strand, within menBat 4095.110 kb on + strand, within menBat 4095.110 kb on + strand, within menBat 4095.110 kb on + strand, within menBat 4095.212 kb on + strandat 4095.212 kb on + strandat 4095.213 kb on - strandat 4095.286 kb on + strandat 4095.287 kb on - strandat 4095.395 kb on + strand, within menCat 4095.455 kb on + strand, within menCat 4095.525 kb on + strand, within menCat 4095.525 kb on + strand, within menCat 4095.565 kb on - strand, within menCat 4095.604 kb on + strand, within menCat 4095.604 kb on + strand, within menCat 4095.724 kb on + strand, within menCat 4095.890 kb on + strand, within menCat 4095.890 kb on + strand, within menCat 4095.890 kb on + strand, within menCat 4095.894 kb on + strand, within menCat 4096.001 kb on + strand, within menCat 4096.001 kb on + strand, within menCat 4096.154 kb on + strandat 4096.217 kb on - strandat 4096.223 kb on + strandat 4096.616 kb on + strand, within menEat 4096.617 kb on - strand, within menEat 4096.617 kb on - strand, within menEat 4096.730 kb on + strand, within menEat 4096.730 kb on + strand, within menEat 4096.746 kb on + strand, within menEat 4096.808 kb on + strand, within menEat 4097.136 kb on + strand, within menEat 4097.136 kb on + strand, within menE

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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4,094,261 + -0.7
4,094,262 - -0.6
4,094,327 - +1.4
4,094,338 + +0.8
4,094,646 + menB NIAGMN_20715 0.36 +0.6
4,094,647 - menB NIAGMN_20715 0.36 +1.8
4,094,687 - menB NIAGMN_20715 0.41 -1.0
4,094,782 + menB NIAGMN_20715 0.52 +1.4
4,094,846 + menB NIAGMN_20715 0.59 +1.0
4,095,110 + menB NIAGMN_20715 0.90 +2.8
4,095,110 + menB NIAGMN_20715 0.90 +0.6
4,095,110 + menB NIAGMN_20715 0.90 +0.2
4,095,212 + -1.0
4,095,212 + +0.5
4,095,213 - -0.2
4,095,286 + +0.8
4,095,287 - +0.8
4,095,395 + menC NIAGMN_20720 0.21 +1.2
4,095,455 + menC NIAGMN_20720 0.27 -0.4
4,095,525 + menC NIAGMN_20720 0.34 +1.8
4,095,525 + menC NIAGMN_20720 0.34 +1.2
4,095,565 - menC NIAGMN_20720 0.38 +1.8
4,095,604 + menC NIAGMN_20720 0.42 +0.1
4,095,604 + menC NIAGMN_20720 0.42 -0.8
4,095,724 + menC NIAGMN_20720 0.55 +0.4
4,095,890 + menC NIAGMN_20720 0.72 +0.2
4,095,890 + menC NIAGMN_20720 0.72 +0.4
4,095,890 + menC NIAGMN_20720 0.72 -0.2
4,095,894 + menC NIAGMN_20720 0.72 +0.1
4,096,001 + menC NIAGMN_20720 0.84 +0.2
4,096,001 + menC NIAGMN_20720 0.84 +0.5
4,096,154 + -2.3
4,096,217 - +1.8
4,096,223 + +1.8
4,096,616 + menE NIAGMN_20725 0.34 +0.8
4,096,617 - menE NIAGMN_20725 0.34 -1.7
4,096,617 - menE NIAGMN_20725 0.34 +0.8
4,096,730 + menE NIAGMN_20725 0.42 -1.0
4,096,730 + menE NIAGMN_20725 0.42 -1.2
4,096,746 + menE NIAGMN_20725 0.44 +1.2
4,096,808 + menE NIAGMN_20725 0.48 -0.6
4,097,136 + menE NIAGMN_20725 0.72 -0.2
4,097,136 + menE NIAGMN_20725 0.72 +0.2

Or see this region's nucleotide sequence