Experiment: EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pheA and NIAGMN_19000 are separated by 98 nucleotides NIAGMN_19000 and raiA are separated by 103 nucleotides raiA and NIAGMN_19010 are separated by 5 nucleotides NIAGMN_19010 and bamD are separated by 229 nucleotides
NIAGMN_18995: pheA - bifunctional chorismate mutase/prephenate dehydratase, at 3,742,649 to 3,743,809
pheA
NIAGMN_19000: NIAGMN_19000 - Leader peptide of chorismate mutase-P-prephenate dehydratase, at 3,743,908 to 3,743,955
_19000
NIAGMN_19005: raiA - ribosome-associated translation inhibitor RaiA, at 3,744,059 to 3,744,400
raiA
NIAGMN_19010: NIAGMN_19010 - Leader peptide, at 3,744,406 to 3,744,441
_19010
NIAGMN_19015: bamD - outer membrane protein assembly factor BamD, at 3,744,671 to 3,745,408
bamD
Position (kb)
3744
3745 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3743.442 kb on + strand, within pheA at 3743.553 kb on - strand, within pheA at 3743.595 kb on + strand, within pheA at 3743.596 kb on - strand, within pheA at 3743.629 kb on + strand, within pheA at 3743.645 kb on + strand, within pheA at 3743.738 kb on + strand at 3743.809 kb on + strand at 3743.861 kb on + strand at 3743.861 kb on + strand at 3743.862 kb on - strand at 3743.968 kb on - strand at 3743.980 kb on + strand at 3743.980 kb on + strand at 3743.991 kb on + strand at 3744.050 kb on + strand at 3744.053 kb on - strand at 3744.053 kb on - strand at 3744.053 kb on - strand at 3744.053 kb on - strand at 3744.061 kb on - strand at 3744.061 kb on - strand at 3744.061 kb on - strand at 3744.143 kb on - strand, within raiA at 3744.189 kb on + strand, within raiA at 3744.190 kb on - strand, within raiA at 3744.190 kb on - strand, within raiA at 3744.194 kb on - strand, within raiA at 3744.194 kb on - strand, within raiA at 3744.194 kb on - strand, within raiA at 3744.194 kb on - strand, within raiA at 3744.194 kb on - strand, within raiA at 3744.194 kb on - strand, within raiA at 3744.205 kb on + strand, within raiA at 3744.230 kb on - strand, within raiA at 3744.235 kb on + strand, within raiA at 3744.235 kb on + strand, within raiA at 3744.236 kb on - strand, within raiA at 3744.287 kb on - strand, within raiA at 3744.407 kb on + strand at 3744.441 kb on + strand at 3744.442 kb on - strand at 3744.442 kb on - strand at 3744.542 kb on + strand at 3744.593 kb on + strand at 3744.673 kb on - strand at 3744.673 kb on - strand at 3744.676 kb on + strand at 3744.677 kb on - strand at 3744.677 kb on - strand at 3744.677 kb on - strand at 3744.677 kb on - strand at 3744.677 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EV240 remove 3,743,442 + pheA NIAGMN_18995 0.68 +0.5 3,743,553 - pheA NIAGMN_18995 0.78 +0.1 3,743,595 + pheA NIAGMN_18995 0.81 +1.1 3,743,596 - pheA NIAGMN_18995 0.82 +0.6 3,743,629 + pheA NIAGMN_18995 0.84 -1.0 3,743,645 + pheA NIAGMN_18995 0.86 -0.3 3,743,738 + -1.1 3,743,809 + -0.0 3,743,861 + +0.1 3,743,861 + +0.6 3,743,862 - +0.1 3,743,968 - +1.5 3,743,980 + +0.0 3,743,980 + -0.2 3,743,991 + -0.4 3,744,050 + +0.7 3,744,053 - +1.2 3,744,053 - -1.0 3,744,053 - -1.9 3,744,053 - -2.2 3,744,061 - +0.3 3,744,061 - +1.7 3,744,061 - +0.1 3,744,143 - raiA NIAGMN_19005 0.25 -0.9 3,744,189 + raiA NIAGMN_19005 0.38 +0.4 3,744,190 - raiA NIAGMN_19005 0.38 +0.0 3,744,190 - raiA NIAGMN_19005 0.38 +0.1 3,744,194 - raiA NIAGMN_19005 0.39 -0.4 3,744,194 - raiA NIAGMN_19005 0.39 +1.7 3,744,194 - raiA NIAGMN_19005 0.39 +0.7 3,744,194 - raiA NIAGMN_19005 0.39 +1.0 3,744,194 - raiA NIAGMN_19005 0.39 -1.1 3,744,194 - raiA NIAGMN_19005 0.39 -0.4 3,744,205 + raiA NIAGMN_19005 0.43 +2.7 3,744,230 - raiA NIAGMN_19005 0.50 -0.4 3,744,235 + raiA NIAGMN_19005 0.51 -0.9 3,744,235 + raiA NIAGMN_19005 0.51 -1.6 3,744,236 - raiA NIAGMN_19005 0.52 -0.9 3,744,287 - raiA NIAGMN_19005 0.67 +0.1 3,744,407 + +0.1 3,744,441 + +0.7 3,744,442 - +0.0 3,744,442 - -1.3 3,744,542 + +1.0 3,744,593 + -0.6 3,744,673 - +0.3 3,744,673 - +1.3 3,744,676 + +1.4 3,744,677 - -0.4 3,744,677 - +0.4 3,744,677 - +1.3 3,744,677 - +0.2 3,744,677 - +0.9
Or see this region's nucleotide sequence