Strain Fitness in Escherichia coli ECRC102 around NIAGMN_18160

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcysH and hokG are separated by 263 nucleotideshokG and cas3 are separated by 191 nucleotides NIAGMN_18150: cysH - phosphoadenosine phosphosulfate reductase, at 3,593,237 to 3,593,971 cysH NIAGMN_18160: hokG - Putative protein HokG, at 3,594,235 to 3,594,387 hokG NIAGMN_18165: cas3 - CRISPR-associated helicase/endonuclease Cas3, at 3,594,579 to 3,597,278 cas3 Position (kb) 3594 3595Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3593.236 kb on + strandat 3593.237 kb on - strandat 3593.237 kb on - strandat 3593.237 kb on - strandat 3593.237 kb on - strandat 3593.237 kb on - strandat 3593.237 kb on - strandat 3593.281 kb on - strandat 3593.379 kb on - strand, within cysHat 3593.379 kb on - strand, within cysHat 3593.379 kb on - strand, within cysHat 3593.382 kb on + strand, within cysHat 3593.383 kb on - strand, within cysHat 3593.477 kb on + strand, within cysHat 3593.496 kb on - strand, within cysHat 3593.504 kb on - strand, within cysHat 3593.546 kb on + strand, within cysHat 3593.627 kb on + strand, within cysHat 3593.677 kb on + strand, within cysHat 3593.768 kb on - strand, within cysHat 3593.805 kb on + strand, within cysHat 3593.807 kb on + strand, within cysHat 3593.807 kb on + strand, within cysHat 3593.813 kb on + strand, within cysHat 3593.861 kb on + strand, within cysHat 3593.973 kb on + strandat 3593.988 kb on + strandat 3594.074 kb on - strandat 3594.082 kb on + strandat 3594.082 kb on + strandat 3594.082 kb on + strandat 3594.083 kb on - strandat 3594.165 kb on + strandat 3594.166 kb on - strandat 3594.193 kb on - strandat 3594.193 kb on - strandat 3594.208 kb on - strandat 3594.239 kb on + strandat 3594.240 kb on - strandat 3594.248 kb on - strandat 3594.266 kb on + strand, within hokGat 3594.266 kb on + strand, within hokGat 3594.266 kb on + strand, within hokGat 3594.266 kb on + strand, within hokGat 3594.266 kb on + strand, within hokGat 3594.267 kb on - strand, within hokGat 3594.269 kb on + strand, within hokGat 3594.269 kb on + strand, within hokGat 3594.269 kb on + strand, within hokGat 3594.270 kb on - strand, within hokGat 3594.279 kb on + strand, within hokGat 3594.279 kb on + strand, within hokGat 3594.279 kb on + strand, within hokGat 3594.279 kb on + strand, within hokGat 3594.280 kb on - strand, within hokGat 3594.280 kb on - strand, within hokGat 3594.317 kb on + strand, within hokGat 3594.317 kb on + strand, within hokGat 3594.317 kb on + strand, within hokGat 3594.318 kb on - strand, within hokGat 3594.318 kb on - strand, within hokGat 3594.346 kb on - strand, within hokGat 3594.385 kb on + strandat 3594.385 kb on + strandat 3594.385 kb on + strandat 3594.385 kb on + strandat 3594.475 kb on + strandat 3594.478 kb on - strandat 3594.497 kb on - strandat 3594.556 kb on - strandat 3594.584 kb on + strandat 3594.730 kb on + strandat 3595.033 kb on + strand, within cas3at 3595.033 kb on + strand, within cas3at 3595.235 kb on + strand, within cas3at 3595.236 kb on - strand, within cas3at 3595.316 kb on + strand, within cas3at 3595.317 kb on - strand, within cas3

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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3,593,236 + +0.8
3,593,237 - +0.4
3,593,237 - -0.6
3,593,237 - -0.8
3,593,237 - -0.6
3,593,237 - -0.8
3,593,237 - -0.8
3,593,281 - -0.6
3,593,379 - cysH NIAGMN_18150 0.19 +1.4
3,593,379 - cysH NIAGMN_18150 0.19 +0.2
3,593,379 - cysH NIAGMN_18150 0.19 +0.3
3,593,382 + cysH NIAGMN_18150 0.20 -2.0
3,593,383 - cysH NIAGMN_18150 0.20 -0.2
3,593,477 + cysH NIAGMN_18150 0.33 -1.9
3,593,496 - cysH NIAGMN_18150 0.35 -0.2
3,593,504 - cysH NIAGMN_18150 0.36 -1.8
3,593,546 + cysH NIAGMN_18150 0.42 -1.0
3,593,627 + cysH NIAGMN_18150 0.53 +1.8
3,593,677 + cysH NIAGMN_18150 0.60 +0.4
3,593,768 - cysH NIAGMN_18150 0.72 +1.2
3,593,805 + cysH NIAGMN_18150 0.77 +0.2
3,593,807 + cysH NIAGMN_18150 0.78 +1.8
3,593,807 + cysH NIAGMN_18150 0.78 +0.6
3,593,813 + cysH NIAGMN_18150 0.78 +0.3
3,593,861 + cysH NIAGMN_18150 0.85 +2.8
3,593,973 + -0.1
3,593,988 + +1.8
3,594,074 - +0.6
3,594,082 + +1.2
3,594,082 + -0.9
3,594,082 + +0.2
3,594,083 - -1.9
3,594,165 + +0.5
3,594,166 - -0.4
3,594,193 - -0.9
3,594,193 - -0.4
3,594,208 - +0.7
3,594,239 + -0.4
3,594,240 - +0.8
3,594,248 - -0.1
3,594,266 + hokG NIAGMN_18160 0.20 +1.8
3,594,266 + hokG NIAGMN_18160 0.20 -0.8
3,594,266 + hokG NIAGMN_18160 0.20 +0.3
3,594,266 + hokG NIAGMN_18160 0.20 -0.6
3,594,266 + hokG NIAGMN_18160 0.20 -1.6
3,594,267 - hokG NIAGMN_18160 0.21 -0.1
3,594,269 + hokG NIAGMN_18160 0.22 +0.3
3,594,269 + hokG NIAGMN_18160 0.22 +1.0
3,594,269 + hokG NIAGMN_18160 0.22 -0.6
3,594,270 - hokG NIAGMN_18160 0.23 -1.4
3,594,279 + hokG NIAGMN_18160 0.29 +0.7
3,594,279 + hokG NIAGMN_18160 0.29 -0.4
3,594,279 + hokG NIAGMN_18160 0.29 -0.0
3,594,279 + hokG NIAGMN_18160 0.29 +0.2
3,594,280 - hokG NIAGMN_18160 0.29 -0.2
3,594,280 - hokG NIAGMN_18160 0.29 -0.6
3,594,317 + hokG NIAGMN_18160 0.54 -0.8
3,594,317 + hokG NIAGMN_18160 0.54 -0.6
3,594,317 + hokG NIAGMN_18160 0.54 -1.0
3,594,318 - hokG NIAGMN_18160 0.54 +0.9
3,594,318 - hokG NIAGMN_18160 0.54 +0.2
3,594,346 - hokG NIAGMN_18160 0.73 -0.9
3,594,385 + +0.1
3,594,385 + -0.4
3,594,385 + -0.2
3,594,385 + +0.3
3,594,475 + +0.7
3,594,478 - -0.7
3,594,497 - -0.7
3,594,556 - +0.5
3,594,584 + +1.5
3,594,730 + -0.1
3,595,033 + cas3 NIAGMN_18165 0.17 -0.5
3,595,033 + cas3 NIAGMN_18165 0.17 -0.3
3,595,235 + cas3 NIAGMN_18165 0.24 +0.8
3,595,236 - cas3 NIAGMN_18165 0.24 +1.0
3,595,316 + cas3 NIAGMN_18165 0.27 +0.0
3,595,317 - cas3 NIAGMN_18165 0.27 -0.8

Or see this region's nucleotide sequence