Experiment: EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt waaO and rfaY are separated by 25 nucleotides rfaY and rfaJ are separated by 24 nucleotides
NIAGMN_13425: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 2,652,758 to 2,653,765
waaO
NIAGMN_13430: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 2,653,791 to 2,654,498
rfaY
NIAGMN_13435: rfaJ - glucosyltransferase, at 2,654,523 to 2,655,536
rfaJ
Position (kb)
2653
2654
2655 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2652.885 kb on + strand, within waaO at 2653.117 kb on + strand, within waaO at 2653.201 kb on + strand, within waaO at 2653.428 kb on + strand, within waaO at 2653.483 kb on + strand, within waaO at 2653.542 kb on + strand, within waaO at 2653.542 kb on + strand, within waaO at 2653.547 kb on + strand, within waaO at 2653.547 kb on + strand, within waaO at 2653.547 kb on + strand, within waaO at 2653.663 kb on + strand, within waaO at 2653.913 kb on + strand, within rfaY at 2654.144 kb on + strand, within rfaY at 2654.144 kb on + strand, within rfaY at 2654.390 kb on + strand, within rfaY at 2654.506 kb on - strand at 2654.719 kb on - strand, within rfaJ at 2654.832 kb on - strand, within rfaJ at 2654.900 kb on - strand, within rfaJ at 2654.995 kb on + strand, within rfaJ at 2655.059 kb on + strand, within rfaJ at 2655.131 kb on - strand, within rfaJ at 2655.160 kb on + strand, within rfaJ at 2655.321 kb on + strand, within rfaJ at 2655.323 kb on + strand, within rfaJ at 2655.323 kb on + strand, within rfaJ at 2655.360 kb on + strand, within rfaJ
Per-strain Table
Position Strand Gene LocusTag Fraction EV240 remove 2,652,885 + waaO NIAGMN_13425 0.13 -2.4 2,653,117 + waaO NIAGMN_13425 0.36 -0.3 2,653,201 + waaO NIAGMN_13425 0.44 -1.3 2,653,428 + waaO NIAGMN_13425 0.66 -2.4 2,653,483 + waaO NIAGMN_13425 0.72 -2.7 2,653,542 + waaO NIAGMN_13425 0.78 -1.1 2,653,542 + waaO NIAGMN_13425 0.78 +0.7 2,653,547 + waaO NIAGMN_13425 0.78 -1.1 2,653,547 + waaO NIAGMN_13425 0.78 -0.7 2,653,547 + waaO NIAGMN_13425 0.78 -2.9 2,653,663 + waaO NIAGMN_13425 0.90 +0.1 2,653,913 + rfaY NIAGMN_13430 0.17 +2.0 2,654,144 + rfaY NIAGMN_13430 0.50 +1.3 2,654,144 + rfaY NIAGMN_13430 0.50 +1.4 2,654,390 + rfaY NIAGMN_13430 0.85 +1.7 2,654,506 - -2.8 2,654,719 - rfaJ NIAGMN_13435 0.19 -0.7 2,654,832 - rfaJ NIAGMN_13435 0.30 -2.6 2,654,900 - rfaJ NIAGMN_13435 0.37 -3.5 2,654,995 + rfaJ NIAGMN_13435 0.47 -0.7 2,655,059 + rfaJ NIAGMN_13435 0.53 -0.3 2,655,131 - rfaJ NIAGMN_13435 0.60 +0.1 2,655,160 + rfaJ NIAGMN_13435 0.63 -3.1 2,655,321 + rfaJ NIAGMN_13435 0.79 -1.3 2,655,323 + rfaJ NIAGMN_13435 0.79 -1.8 2,655,323 + rfaJ NIAGMN_13435 0.79 -3.7 2,655,360 + rfaJ NIAGMN_13435 0.83 -3.5
Or see this region's nucleotide sequence