Strain Fitness in Escherichia coli ECRC102 around NIAGMN_08125

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmtnN and btuF overlap by 8 nucleotidesbtuF and yadS are separated by 37 nucleotidesyadS and erpA are separated by 46 nucleotideserpA and yadW overlap by 8 nucleotidesyadW and clcA are separated by 23 nucleotides NIAGMN_08115: mtnN - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, at 1,558,692 to 1,559,390 mtnN NIAGMN_08120: btuF - vitamin B12 ABC transporter substrate-binding protein BtuF, at 1,559,383 to 1,560,183 btuF NIAGMN_08125: yadS - UPF0126 inner membrane protein YadS, at 1,560,221 to 1,560,844 yadS NIAGMN_08130: erpA - iron-sulfur cluster insertion protein ErpA, at 1,560,891 to 1,561,235 erpA NIAGMN_08135: yadW - Protein YadW, at 1,561,228 to 1,561,293 yadW NIAGMN_08140: clcA - H(+)/Cl(-) exchange transporter ClcA, at 1,561,317 to 1,562,738 clcA Position (kb) 1560 1561Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1559.365 kb on + strandat 1559.388 kb on + strandat 1559.389 kb on - strandat 1559.389 kb on - strandat 1559.531 kb on - strand, within btuFat 1559.646 kb on + strand, within btuFat 1559.647 kb on - strand, within btuFat 1559.688 kb on - strand, within btuFat 1559.738 kb on + strand, within btuFat 1559.918 kb on + strand, within btuFat 1559.918 kb on + strand, within btuFat 1559.996 kb on + strand, within btuFat 1559.996 kb on + strand, within btuFat 1559.997 kb on - strand, within btuFat 1560.090 kb on + strand, within btuFat 1560.132 kb on + strandat 1560.132 kb on + strandat 1560.132 kb on + strandat 1560.132 kb on + strandat 1560.132 kb on + strandat 1560.132 kb on + strandat 1560.133 kb on - strandat 1560.133 kb on - strandat 1560.177 kb on - strandat 1560.197 kb on + strandat 1560.241 kb on + strandat 1560.241 kb on + strandat 1560.242 kb on - strandat 1560.243 kb on + strandat 1560.244 kb on - strandat 1560.244 kb on - strandat 1560.258 kb on + strandat 1560.298 kb on + strand, within yadSat 1560.475 kb on - strand, within yadSat 1560.583 kb on + strand, within yadSat 1560.583 kb on + strand, within yadSat 1560.584 kb on - strand, within yadSat 1560.654 kb on + strand, within yadSat 1560.713 kb on + strand, within yadSat 1560.748 kb on + strand, within yadSat 1560.772 kb on - strand, within yadSat 1560.805 kb on + strandat 1560.805 kb on + strandat 1560.805 kb on + strandat 1560.805 kb on + strandat 1560.805 kb on + strandat 1560.892 kb on + strandat 1560.893 kb on - strandat 1560.897 kb on - strandat 1560.900 kb on - strandat 1560.900 kb on - strandat 1561.282 kb on - strand, within yadWat 1561.282 kb on - strand, within yadWat 1561.282 kb on - strand, within yadWat 1561.405 kb on + strandat 1561.405 kb on + strandat 1561.406 kb on - strandat 1561.408 kb on - strandat 1561.438 kb on - strandat 1561.438 kb on - strandat 1561.438 kb on - strandat 1561.460 kb on + strand, within clcAat 1561.483 kb on + strand, within clcAat 1561.484 kb on - strand, within clcAat 1561.484 kb on - strand, within clcAat 1561.484 kb on - strand, within clcAat 1561.487 kb on + strand, within clcAat 1561.569 kb on - strand, within clcAat 1561.595 kb on - strand, within clcAat 1561.647 kb on - strand, within clcAat 1561.647 kb on - strand, within clcAat 1561.704 kb on + strand, within clcAat 1561.704 kb on + strand, within clcAat 1561.704 kb on + strand, within clcAat 1561.705 kb on - strand, within clcAat 1561.705 kb on - strand, within clcAat 1561.705 kb on - strand, within clcAat 1561.734 kb on - strand, within clcAat 1561.734 kb on - strand, within clcAat 1561.770 kb on - strand, within clcAat 1561.787 kb on + strand, within clcAat 1561.788 kb on - strand, within clcA

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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1,559,365 + -0.2
1,559,388 + +0.0
1,559,389 - +0.1
1,559,389 - -0.1
1,559,531 - btuF NIAGMN_08120 0.18 -1.5
1,559,646 + btuF NIAGMN_08120 0.33 -0.5
1,559,647 - btuF NIAGMN_08120 0.33 +0.7
1,559,688 - btuF NIAGMN_08120 0.38 -0.9
1,559,738 + btuF NIAGMN_08120 0.44 -1.1
1,559,918 + btuF NIAGMN_08120 0.67 +1.0
1,559,918 + btuF NIAGMN_08120 0.67 +0.4
1,559,996 + btuF NIAGMN_08120 0.77 -0.2
1,559,996 + btuF NIAGMN_08120 0.77 +0.7
1,559,997 - btuF NIAGMN_08120 0.77 -0.1
1,560,090 + btuF NIAGMN_08120 0.88 +1.1
1,560,132 + -0.1
1,560,132 + -0.4
1,560,132 + -0.6
1,560,132 + -0.1
1,560,132 + +0.3
1,560,132 + -1.6
1,560,133 - +0.5
1,560,133 - -1.4
1,560,177 - -0.1
1,560,197 + +0.4
1,560,241 + -0.6
1,560,241 + -0.7
1,560,242 - +0.1
1,560,243 + -0.6
1,560,244 - -1.9
1,560,244 - +0.7
1,560,258 + -1.0
1,560,298 + yadS NIAGMN_08125 0.12 -1.4
1,560,475 - yadS NIAGMN_08125 0.41 +0.6
1,560,583 + yadS NIAGMN_08125 0.58 -0.6
1,560,583 + yadS NIAGMN_08125 0.58 -0.5
1,560,584 - yadS NIAGMN_08125 0.58 +0.4
1,560,654 + yadS NIAGMN_08125 0.69 -0.2
1,560,713 + yadS NIAGMN_08125 0.79 -0.1
1,560,748 + yadS NIAGMN_08125 0.84 +1.1
1,560,772 - yadS NIAGMN_08125 0.88 -1.3
1,560,805 + -0.1
1,560,805 + -2.2
1,560,805 + +0.7
1,560,805 + +1.4
1,560,805 + -0.4
1,560,892 + -0.7
1,560,893 - -1.3
1,560,897 - -0.1
1,560,900 - -0.6
1,560,900 - -2.4
1,561,282 - yadW NIAGMN_08135 0.82 -0.2
1,561,282 - yadW NIAGMN_08135 0.82 -1.8
1,561,282 - yadW NIAGMN_08135 0.82 +1.2
1,561,405 + -0.3
1,561,405 + -0.4
1,561,406 - -0.1
1,561,408 - +0.2
1,561,438 - +0.4
1,561,438 - +2.7
1,561,438 - -0.8
1,561,460 + clcA NIAGMN_08140 0.10 -0.1
1,561,483 + clcA NIAGMN_08140 0.12 -0.9
1,561,484 - clcA NIAGMN_08140 0.12 -1.3
1,561,484 - clcA NIAGMN_08140 0.12 +0.9
1,561,484 - clcA NIAGMN_08140 0.12 +1.7
1,561,487 + clcA NIAGMN_08140 0.12 +0.4
1,561,569 - clcA NIAGMN_08140 0.18 -0.7
1,561,595 - clcA NIAGMN_08140 0.20 -0.9
1,561,647 - clcA NIAGMN_08140 0.23 -3.2
1,561,647 - clcA NIAGMN_08140 0.23 -0.6
1,561,704 + clcA NIAGMN_08140 0.27 +0.5
1,561,704 + clcA NIAGMN_08140 0.27 -0.4
1,561,704 + clcA NIAGMN_08140 0.27 -0.2
1,561,705 - clcA NIAGMN_08140 0.27 -0.0
1,561,705 - clcA NIAGMN_08140 0.27 -0.1
1,561,705 - clcA NIAGMN_08140 0.27 -1.1
1,561,734 - clcA NIAGMN_08140 0.29 +0.1
1,561,734 - clcA NIAGMN_08140 0.29 +0.3
1,561,770 - clcA NIAGMN_08140 0.32 -0.4
1,561,787 + clcA NIAGMN_08140 0.33 -0.3
1,561,788 - clcA NIAGMN_08140 0.33 +0.0

Or see this region's nucleotide sequence