Strain Fitness in Escherichia coli ECRC102 around NIAGMN_03445

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybdN and NIAGMN_03445 are separated by 500 nucleotidesNIAGMN_03445 and NIAGMN_03450 overlap by 4 nucleotidesNIAGMN_03450 and fhaC are separated by 240 nucleotides NIAGMN_03440: ybdN - phosphoadenosine phosphosulfate reductase, at 615,502 to 616,734 ybdN NIAGMN_03445: NIAGMN_03445 - helicase, at 617,235 to 619,403 _03445 NIAGMN_03450: NIAGMN_03450 - Putative UvrD/REP helicase-like protein, at 619,400 to 619,915 _03450 NIAGMN_03455: fhaC - ShlB/FhaC/HecB family hemolysin secretion/activation protein, at 620,156 to 621,202 fhaC Position (kb) 617 618 619 620Strain fitness (log2 ratio) -1 0 1 2at 616.476 kb on - strand, within ybdNat 616.498 kb on - strand, within ybdNat 616.634 kb on - strandat 616.927 kb on - strandat 617.120 kb on + strandat 617.428 kb on + strandat 617.429 kb on - strandat 617.460 kb on - strand, within NIAGMN_03445at 617.470 kb on - strand, within NIAGMN_03445at 617.763 kb on - strand, within NIAGMN_03445at 618.015 kb on - strand, within NIAGMN_03445at 618.015 kb on - strand, within NIAGMN_03445at 618.136 kb on - strand, within NIAGMN_03445at 618.275 kb on + strand, within NIAGMN_03445at 618.398 kb on + strand, within NIAGMN_03445at 618.501 kb on - strand, within NIAGMN_03445at 618.878 kb on - strand, within NIAGMN_03445at 619.293 kb on - strandat 620.031 kb on + strandat 620.104 kb on + strandat 620.185 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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616,476 - ybdN NIAGMN_03440 0.79 +0.2
616,498 - ybdN NIAGMN_03440 0.81 +0.5
616,634 - +0.2
616,927 - -1.0
617,120 + -1.3
617,428 + +0.7
617,429 - -0.3
617,460 - NIAGMN_03445 0.10 -0.4
617,470 - NIAGMN_03445 0.11 +1.7
617,763 - NIAGMN_03445 0.24 +0.1
618,015 - NIAGMN_03445 0.36 -0.6
618,015 - NIAGMN_03445 0.36 +2.0
618,136 - NIAGMN_03445 0.42 +1.0
618,275 + NIAGMN_03445 0.48 +1.7
618,398 + NIAGMN_03445 0.54 +0.4
618,501 - NIAGMN_03445 0.58 -0.9
618,878 - NIAGMN_03445 0.76 +1.3
619,293 - -1.1
620,031 + +0.4
620,104 + +0.5
620,185 + -1.0

Or see this region's nucleotide sequence